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Hypoglycemic effect of basil (Ocimum basilicum) aqueous extract is mediated through inhibition of α-glucosidase and α-amylase activities: an in vitro study.
Toxicol Ind Health. 2012 Feb; 28(1):42-50.TI

Abstract

The present study investigated the in vitro hypoglycemic activity of basil (Ocimum basilicum) aqueous extract. Preliminary phytochemical screening of the extract revealed the presence of reducing sugars, cardiac glycosides, tannins, saponins, glycosides, flavonoids and steroids. The total polyphenols content (TPC), flavonoids content (FC), percentage diphenylpicrylhydrazyl (DPPH(·)) radical inhibition and total antioxidant status (TAS) were estimated. The FC was 41 ± 2.2 rutin/g dry extract, the TPC was 146 ± 5.26 mg catechin/g dry extract and the TAS was 5.12 ± 0.7 mmol/L. The %DPPH(·) free radical inhibition was 60%, 54%, 49% and 43%, respectively, for different extract concentrations; 20, 18.2, 16.3 and 14.5 mg/ml, respectively. The extract elicited significant dose-dependent pattern against rat intestinal sucrase (RIS; IC(50) = 36.72 mg/ml), rat intestinal maltase (RIM; IC(50) = 21.31 mg/ml) and porcine pancreatic α-amylase (PPA; IC(50) = 42.50 mg/ml) inhibitory activities. The inhibition was greater against maltase compared with sucrase. These effects may be attributed to the high TPC and FC levels. The linear regression analysis revealed strong significant positive correlations between %DPPH(·) radical inhibition and each of %RIS, %RIM and %PPA inhibiting activity. Also, strong significant positive correlations between %RIS and either %RIM or %PPA inhibition activity were observed. We concluded therefore that basil aqueous extract via antioxidant and possibly α-glucosidase and α-amylase inhibiting activities, offered positive benefits to control diabetes.

Authors+Show Affiliations

Medical Laboratories Technology Department, Faculty of Applied Medical Sciences, Taibah University, Madinah, Saudi Arabia. hesham_elbeshbishy@hotmail.comNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

21636683

Citation

El-Beshbishy, Ha, and Sa Bahashwan. "Hypoglycemic Effect of Basil (Ocimum Basilicum) Aqueous Extract Is Mediated Through Inhibition of Α-glucosidase and Α-amylase Activities: an in Vitro Study." Toxicology and Industrial Health, vol. 28, no. 1, 2012, pp. 42-50.
El-Beshbishy H, Bahashwan S. Hypoglycemic effect of basil (Ocimum basilicum) aqueous extract is mediated through inhibition of α-glucosidase and α-amylase activities: an in vitro study. Toxicol Ind Health. 2012;28(1):42-50.
El-Beshbishy, H., & Bahashwan, S. (2012). Hypoglycemic effect of basil (Ocimum basilicum) aqueous extract is mediated through inhibition of α-glucosidase and α-amylase activities: an in vitro study. Toxicology and Industrial Health, 28(1), 42-50. https://doi.org/10.1177/0748233711403193
El-Beshbishy H, Bahashwan S. Hypoglycemic Effect of Basil (Ocimum Basilicum) Aqueous Extract Is Mediated Through Inhibition of Α-glucosidase and Α-amylase Activities: an in Vitro Study. Toxicol Ind Health. 2012;28(1):42-50. PubMed PMID: 21636683.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hypoglycemic effect of basil (Ocimum basilicum) aqueous extract is mediated through inhibition of α-glucosidase and α-amylase activities: an in vitro study. AU - El-Beshbishy,Ha, AU - Bahashwan,Sa, Y1 - 2011/06/02/ PY - 2011/6/4/entrez PY - 2011/6/4/pubmed PY - 2012/8/15/medline SP - 42 EP - 50 JF - Toxicology and industrial health JO - Toxicol Ind Health VL - 28 IS - 1 N2 - The present study investigated the in vitro hypoglycemic activity of basil (Ocimum basilicum) aqueous extract. Preliminary phytochemical screening of the extract revealed the presence of reducing sugars, cardiac glycosides, tannins, saponins, glycosides, flavonoids and steroids. The total polyphenols content (TPC), flavonoids content (FC), percentage diphenylpicrylhydrazyl (DPPH(·)) radical inhibition and total antioxidant status (TAS) were estimated. The FC was 41 ± 2.2 rutin/g dry extract, the TPC was 146 ± 5.26 mg catechin/g dry extract and the TAS was 5.12 ± 0.7 mmol/L. The %DPPH(·) free radical inhibition was 60%, 54%, 49% and 43%, respectively, for different extract concentrations; 20, 18.2, 16.3 and 14.5 mg/ml, respectively. The extract elicited significant dose-dependent pattern against rat intestinal sucrase (RIS; IC(50) = 36.72 mg/ml), rat intestinal maltase (RIM; IC(50) = 21.31 mg/ml) and porcine pancreatic α-amylase (PPA; IC(50) = 42.50 mg/ml) inhibitory activities. The inhibition was greater against maltase compared with sucrase. These effects may be attributed to the high TPC and FC levels. The linear regression analysis revealed strong significant positive correlations between %DPPH(·) radical inhibition and each of %RIS, %RIM and %PPA inhibiting activity. Also, strong significant positive correlations between %RIS and either %RIM or %PPA inhibition activity were observed. We concluded therefore that basil aqueous extract via antioxidant and possibly α-glucosidase and α-amylase inhibiting activities, offered positive benefits to control diabetes. SN - 1477-0393 UR - https://www.unboundmedicine.com/medline/citation/21636683/Hypoglycemic_effect_of_basil__Ocimum_basilicum__aqueous_extract_is_mediated_through_inhibition_of_α_glucosidase_and_α_amylase_activities:_an_in_vitro_study_ L2 - https://journals.sagepub.com/doi/10.1177/0748233711403193?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -