Citation
da Silva, David, et al. "Evaluation of Combined Matrix-assisted Laser Desorption/ionization Time-of-flight and Matrix-assisted Laser Desorption/ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry Experiments for Peptide Mass Fingerprinting Analysis." Rapid Communications in Mass Spectrometry : RCM, vol. 25, no. 13, 2011, pp. 1881-92.
da Silva D, Wasselin T, Carré V, et al. Evaluation of combined matrix-assisted laser desorption/ionization time-of-flight and matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry experiments for peptide mass fingerprinting analysis. Rapid Commun Mass Spectrom. 2011;25(13):1881-92.
da Silva, D., Wasselin, T., Carré, V., Chaimbault, P., Bezdetnaya, L., Maunit, B., & Muller, J. F. (2011). Evaluation of combined matrix-assisted laser desorption/ionization time-of-flight and matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry experiments for peptide mass fingerprinting analysis. Rapid Communications in Mass Spectrometry : RCM, 25(13), 1881-92. https://doi.org/10.1002/rcm.5057
da Silva D, et al. Evaluation of Combined Matrix-assisted Laser Desorption/ionization Time-of-flight and Matrix-assisted Laser Desorption/ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry Experiments for Peptide Mass Fingerprinting Analysis. Rapid Commun Mass Spectrom. 2011 Jul 15;25(13):1881-92. PubMed PMID: 21638364.
TY - JOUR
T1 - Evaluation of combined matrix-assisted laser desorption/ionization time-of-flight and matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry experiments for peptide mass fingerprinting analysis.
AU - da Silva,David,
AU - Wasselin,Thierry,
AU - Carré,Vincent,
AU - Chaimbault,Patrick,
AU - Bezdetnaya,Lina,
AU - Maunit,Benoît,
AU - Muller,Jean-François,
PY - 2011/6/4/entrez
PY - 2011/6/4/pubmed
PY - 2011/9/2/medline
SP - 1881
EP - 92
JF - Rapid communications in mass spectrometry : RCM
JO - Rapid Commun Mass Spectrom
VL - 25
IS - 13
N2 - Peptide Mass Fingerprinting (PMF) is still of significant interest in proteomics because it allows a large number of complex samples to be rapidly screened and characterized. The main part of post-translational modifications is generally preserved. In some specific cases, PMF suffers from ambiguous or unsuccessful identification. In order to improve its reliability, a combined approach using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) and matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry (MALDI-FTICRMS) was evaluated. The study was carried out on bovine serum albumin (BSA) digest. The influence of several important parameters (the matrix, the sample preparation method, the amount of the analyte) on the MOWSE score and the protein sequence coverage were evaluated to allow the identification of specific effects. A careful investigation of the sequence coverage obtained by each kind of experiment ensured the detection of specific peptides for each experimental condition. Results highlighted that DHB-FTICRMS and DHB- or CHCA-TOFMS are the most suited combinations of experimental conditions to achieve PMF analysis. The association (convolution) of the data obtained by each of these techniques ensured a significant increase in the MOWSE score and the protein sequence coverage.
SN - 1097-0231
UR - https://www.unboundmedicine.com/medline/citation/21638364/Evaluation_of_combined_matrix_assisted_laser_desorption/ionization_time_of_flight_and_matrix_assisted_laser_desorption/ionization_Fourier_transform_ion_cyclotron_resonance_mass_spectrometry_experiments_for_peptide_mass_fingerprinting_analysis_
L2 - https://doi.org/10.1002/rcm.5057
DB - PRIME
DP - Unbound Medicine
ER -
