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Label-free electrochemical aptasensor for thrombin detection based on the nafion@graphene as platform.
Bioprocess Biosyst Eng. 2011 Nov; 34(9):1081-5.BB

Abstract

A sensitive label-free electrochemical aptasensor was successfully fabricated for thrombin detection with nafion@graphene as platform. With electrostatic interaction between nafion and methylene blue (MB), positive charged MB was successfully assembled on nafion@graphene modified electrode surface, which provided amounts of redox probes for electrochemical aptasensor. In the presence of thrombin, the thrombin aptamer (TBA) on the electrode surface would catch the target on the electrode interface, which made a barrier for electrons and inhibits the electro-transfer, resulting in the decreased differential pulse voltammetry signals of MB. As a result, the proposed approach showed a high sensitivity and a wider linearity to thrombin in the range 0.01-50 nM with a detection limit of 6 pM.

Authors+Show Affiliations

College of Chemistry and Chemical Engineering, Yibin University, Yibin, 644000, Sichuan, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21643688

Citation

Sun, Tao, et al. "Label-free Electrochemical Aptasensor for Thrombin Detection Based On the Nafion@graphene as Platform." Bioprocess and Biosystems Engineering, vol. 34, no. 9, 2011, pp. 1081-5.
Sun T, Wang L, Li N, et al. Label-free electrochemical aptasensor for thrombin detection based on the nafion@graphene as platform. Bioprocess Biosyst Eng. 2011;34(9):1081-5.
Sun, T., Wang, L., Li, N., & Gan, X. (2011). Label-free electrochemical aptasensor for thrombin detection based on the nafion@graphene as platform. Bioprocess and Biosystems Engineering, 34(9), 1081-5. https://doi.org/10.1007/s00449-011-0558-3
Sun T, et al. Label-free Electrochemical Aptasensor for Thrombin Detection Based On the Nafion@graphene as Platform. Bioprocess Biosyst Eng. 2011;34(9):1081-5. PubMed PMID: 21643688.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Label-free electrochemical aptasensor for thrombin detection based on the nafion@graphene as platform. AU - Sun,Tao, AU - Wang,Li, AU - Li,Na, AU - Gan,Xianxue, Y1 - 2011/06/04/ PY - 2011/03/07/received PY - 2011/05/21/accepted PY - 2011/6/7/entrez PY - 2011/6/7/pubmed PY - 2012/3/6/medline SP - 1081 EP - 5 JF - Bioprocess and biosystems engineering JO - Bioprocess Biosyst Eng VL - 34 IS - 9 N2 - A sensitive label-free electrochemical aptasensor was successfully fabricated for thrombin detection with nafion@graphene as platform. With electrostatic interaction between nafion and methylene blue (MB), positive charged MB was successfully assembled on nafion@graphene modified electrode surface, which provided amounts of redox probes for electrochemical aptasensor. In the presence of thrombin, the thrombin aptamer (TBA) on the electrode surface would catch the target on the electrode interface, which made a barrier for electrons and inhibits the electro-transfer, resulting in the decreased differential pulse voltammetry signals of MB. As a result, the proposed approach showed a high sensitivity and a wider linearity to thrombin in the range 0.01-50 nM with a detection limit of 6 pM. SN - 1615-7605 UR - https://www.unboundmedicine.com/medline/citation/21643688/Label_free_electrochemical_aptasensor_for_thrombin_detection_based_on_the_nafion@graphene_as_platform_ L2 - https://dx.doi.org/10.1007/s00449-011-0558-3 DB - PRIME DP - Unbound Medicine ER -