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Development of a novel and efficient strategy for practical identification of Pyrus spp (Rosaceae) cultivars using RAPD fingerprints.
Genet Mol Res. 2011 May 24; 10(2):932-42.GM

Abstract

Accurate and reliable cultivar identification of crop species is essential to guarantee plant material identity for purposes of registration, cultivar protection and production. To facilitate identification of plant cultivars, we developed a novel strategy for efficient recording of DNA molecular fingerprints in genotyped plant individuals. These fingerprints can be used as efficient referential information for quick plant identification. We made a random amplified polymorphic DNA (RAPD) marker analysis of 68 pear cultivars. All pear genotypes could be distinguished by a combination of eight 11-mer primers. The efficiency of the method was further verified by correct identification of four cultivars randomly chosen from the initial 68. The advantages of this identification include use of fewer primers and ease of cultivar separation by the corresponding primers marked on the cultivar identification diagram. The cultivar identification diagram can efficiently serve for pear cultivar identification by readily providing the information needed to separate cultivars. To the best of our knowledge, this is the most efficient strategy for identification of plant varieties using DNA markers; it could be employed for the development of the pear industry and for the utilization of DNA markers to identify other plant species.

Authors+Show Affiliations

Institute of Horticultural, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu, PR China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21644210

Citation

Lin, J, et al. "Development of a Novel and Efficient Strategy for Practical Identification of Pyrus Spp (Rosaceae) Cultivars Using RAPD Fingerprints." Genetics and Molecular Research : GMR, vol. 10, no. 2, 2011, pp. 932-42.
Lin J, Wang XC, Chang YH, et al. Development of a novel and efficient strategy for practical identification of Pyrus spp (Rosaceae) cultivars using RAPD fingerprints. Genet Mol Res. 2011;10(2):932-42.
Lin, J., Wang, X. C., Chang, Y. H., & Fang, J. G. (2011). Development of a novel and efficient strategy for practical identification of Pyrus spp (Rosaceae) cultivars using RAPD fingerprints. Genetics and Molecular Research : GMR, 10(2), 932-42. https://doi.org/10.4238/vol10-2gmr1097
Lin J, et al. Development of a Novel and Efficient Strategy for Practical Identification of Pyrus Spp (Rosaceae) Cultivars Using RAPD Fingerprints. Genet Mol Res. 2011 May 24;10(2):932-42. PubMed PMID: 21644210.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of a novel and efficient strategy for practical identification of Pyrus spp (Rosaceae) cultivars using RAPD fingerprints. AU - Lin,J, AU - Wang,X C, AU - Chang,Y H, AU - Fang,J G, Y1 - 2011/05/24/ PY - 2011/6/7/entrez PY - 2011/6/7/pubmed PY - 2011/10/1/medline SP - 932 EP - 42 JF - Genetics and molecular research : GMR JO - Genet Mol Res VL - 10 IS - 2 N2 - Accurate and reliable cultivar identification of crop species is essential to guarantee plant material identity for purposes of registration, cultivar protection and production. To facilitate identification of plant cultivars, we developed a novel strategy for efficient recording of DNA molecular fingerprints in genotyped plant individuals. These fingerprints can be used as efficient referential information for quick plant identification. We made a random amplified polymorphic DNA (RAPD) marker analysis of 68 pear cultivars. All pear genotypes could be distinguished by a combination of eight 11-mer primers. The efficiency of the method was further verified by correct identification of four cultivars randomly chosen from the initial 68. The advantages of this identification include use of fewer primers and ease of cultivar separation by the corresponding primers marked on the cultivar identification diagram. The cultivar identification diagram can efficiently serve for pear cultivar identification by readily providing the information needed to separate cultivars. To the best of our knowledge, this is the most efficient strategy for identification of plant varieties using DNA markers; it could be employed for the development of the pear industry and for the utilization of DNA markers to identify other plant species. SN - 1676-5680 UR - https://www.unboundmedicine.com/medline/citation/21644210/Development_of_a_novel_and_efficient_strategy_for_practical_identification_of_Pyrus_spp__Rosaceae__cultivars_using_RAPD_fingerprints_ L2 - http://www.geneticsmr.com/articles/1147 DB - PRIME DP - Unbound Medicine ER -