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Platinum-gold alloy nanoparticles and horseradish peroxidase functionalized nanocomposite as a trace label for ultrasensitive electrochemical detection of thrombin.
Anal Chim Acta. 2011 Jul 18; 698(1-2):14-9.AC

Abstract

A novel tracer, platinum-gold alloy nanoparticles (Pt-AuNPs) and horseradish peroxidase (HRP) functionalized single-walled carbon nanotubes (SWCNTs) composite, is employed to label the secondary thrombin aptamer for constructing an ultrasensitive electrochemical aptasensor. Thionine, immobilized on functionalized SWCNTs, provides a pair of distinguished redox peak for electrochemical detection. Both the high-content Pt-AuNPs and HRP on SWCNTs amplify the electrochemical signal of thionine through electrocatalytic reduction of H(2)O(2). Differential pulse voltammetry (DPV) is employed to detect thrombin with different concentrations. The reduction peak current is logarithmically related to the concentration of thrombin in an extremely wide range from 10 fM to 5 nM with a detection limit of 3.6 fM. The dual signal amplification of Pt-AuNPs and HRP functionalized nanocomposite provides a promising way for ultrasensitive assay in electrochemical aptasensors.

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Authors+Show Affiliations

Bai L
Education Ministry Key Laboratory on Luminescence and Real-Time Analysis, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, People's Republic of China.
Yuan R
No affiliation info available
Chai Y
No affiliation info available
Yuan Y
No affiliation info available
Mao L
No affiliation info available
Wang Y
No affiliation info available

MeSH

AlloysAptamers, NucleotideElectrochemical TechniquesElectrochemistryGoldHorseradish PeroxidaseLimit of DetectionMetal NanoparticlesNanocompositesNanotubes, CarbonPlatinumReproducibility of ResultsThrombin

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21645654

Citation

Bai, Lijuan, et al. "Platinum-gold Alloy Nanoparticles and Horseradish Peroxidase Functionalized Nanocomposite as a Trace Label for Ultrasensitive Electrochemical Detection of Thrombin." Analytica Chimica Acta, vol. 698, no. 1-2, 2011, pp. 14-9.
Bai L, Yuan R, Chai Y, et al. Platinum-gold alloy nanoparticles and horseradish peroxidase functionalized nanocomposite as a trace label for ultrasensitive electrochemical detection of thrombin. Anal Chim Acta. 2011;698(1-2):14-9.
Bai, L., Yuan, R., Chai, Y., Yuan, Y., Mao, L., & Wang, Y. (2011). Platinum-gold alloy nanoparticles and horseradish peroxidase functionalized nanocomposite as a trace label for ultrasensitive electrochemical detection of thrombin. Analytica Chimica Acta, 698(1-2), 14-9. https://doi.org/10.1016/j.aca.2011.04.044
Bai L, et al. Platinum-gold Alloy Nanoparticles and Horseradish Peroxidase Functionalized Nanocomposite as a Trace Label for Ultrasensitive Electrochemical Detection of Thrombin. Anal Chim Acta. 2011 Jul 18;698(1-2):14-9. PubMed PMID: 21645654.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Platinum-gold alloy nanoparticles and horseradish peroxidase functionalized nanocomposite as a trace label for ultrasensitive electrochemical detection of thrombin. AU - Bai,Lijuan, AU - Yuan,Ruo, AU - Chai,Yaqin, AU - Yuan,Yali, AU - Mao,Li, AU - Wang,Yan, Y1 - 2011/05/05/ PY - 2010/12/01/received PY - 2011/04/22/revised PY - 2011/04/23/accepted PY - 2011/6/8/entrez PY - 2011/6/8/pubmed PY - 2011/9/29/medline SP - 14 EP - 9 JF - Analytica chimica acta JO - Anal Chim Acta VL - 698 IS - 1-2 N2 - A novel tracer, platinum-gold alloy nanoparticles (Pt-AuNPs) and horseradish peroxidase (HRP) functionalized single-walled carbon nanotubes (SWCNTs) composite, is employed to label the secondary thrombin aptamer for constructing an ultrasensitive electrochemical aptasensor. Thionine, immobilized on functionalized SWCNTs, provides a pair of distinguished redox peak for electrochemical detection. Both the high-content Pt-AuNPs and HRP on SWCNTs amplify the electrochemical signal of thionine through electrocatalytic reduction of H(2)O(2). Differential pulse voltammetry (DPV) is employed to detect thrombin with different concentrations. The reduction peak current is logarithmically related to the concentration of thrombin in an extremely wide range from 10 fM to 5 nM with a detection limit of 3.6 fM. The dual signal amplification of Pt-AuNPs and HRP functionalized nanocomposite provides a promising way for ultrasensitive assay in electrochemical aptasensors. SN - 1873-4324 UR - https://www.unboundmedicine.com/medline/citation/21645654/Platinum_gold_alloy_nanoparticles_and_horseradish_peroxidase_functionalized_nanocomposite_as_a_trace_label_for_ultrasensitive_electrochemical_detection_of_thrombin_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-2670(11)00599-X DB - PRIME DP - Unbound Medicine ER -