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Fast gradient high performance liquid chromatography method with UV detection for simultaneous determination of seven angiotensin converting enzyme inhibitors together with hydrochlorothiazide in pharmaceutical dosage forms and spiked human plasma and urine.
Talanta. 2011 Jul 15; 85(1):123-9.T

Abstract

The development of a reversed phase liquid chromatographic method for the simultaneous determination of seven angiotensin converting enzyme (ACE) inhibitors; five drugs namely benazepril HCl (BZL), enalapril maleate (ENL), fosinopril sodium (FSP), lisinopril (LSP) and ramipril (RMP) and two metabolites captopril disulfide (CPD) and enalaprilat (ENT) together with hydrochlorothiazide (HCT) is described. The method can serve as a substitute for many published papers for the analysis of the targeted compounds with or without hydrochloothiazide in pharmaceutical formulations as well as in spiked human plasma and urine samples. The method utilizes a simple gradient procedure for the separation in a 11 min run time using acetonitrile aqueous ammonia buffer (pH 9) solution and an Extend RP-C18 (25 μm particle size, 4.6 mm×250 mm, Agilent) HPLC column. The effluent was monitored on a UV detector at 215 nm. The effect of pH, solvent strength and analysis time on the peak shape and quantification were carefully studied in order to optimize the method. Adopting the proposed procedure, the analytes produce well-shaped peaks with good linear relationship over the investigated concentration ranges. The limits of detection (LOD) and limits of quantification (LOQ) from standard drug solutions lie in the range of 17-64 and 56-212 ng mL(-1), respectively. Correlation coefficient values (r) higher than 0.997 were obtained for all the studied drugs in spiked human plasma and urine samples. The intra-day and inter-day precision of the method was evaluated with relative standard deviation values being satisfactory for their purposed analysis. The method was validated with respect to specificity, recovery, accuracy, precision and linearity.

Authors+Show Affiliations

Department of Chemistry, Xixi Campus, Zhejiang University, Hangzhou 310028, China. faelsepai@yahoo.comNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21645680

Citation

Elsebaei, Fawzi, and Yan Zhu. "Fast Gradient High Performance Liquid Chromatography Method With UV Detection for Simultaneous Determination of Seven Angiotensin Converting Enzyme Inhibitors Together With Hydrochlorothiazide in Pharmaceutical Dosage Forms and Spiked Human Plasma and Urine." Talanta, vol. 85, no. 1, 2011, pp. 123-9.
Elsebaei F, Zhu Y. Fast gradient high performance liquid chromatography method with UV detection for simultaneous determination of seven angiotensin converting enzyme inhibitors together with hydrochlorothiazide in pharmaceutical dosage forms and spiked human plasma and urine. Talanta. 2011;85(1):123-9.
Elsebaei, F., & Zhu, Y. (2011). Fast gradient high performance liquid chromatography method with UV detection for simultaneous determination of seven angiotensin converting enzyme inhibitors together with hydrochlorothiazide in pharmaceutical dosage forms and spiked human plasma and urine. Talanta, 85(1), 123-9. https://doi.org/10.1016/j.talanta.2011.03.037
Elsebaei F, Zhu Y. Fast Gradient High Performance Liquid Chromatography Method With UV Detection for Simultaneous Determination of Seven Angiotensin Converting Enzyme Inhibitors Together With Hydrochlorothiazide in Pharmaceutical Dosage Forms and Spiked Human Plasma and Urine. Talanta. 2011 Jul 15;85(1):123-9. PubMed PMID: 21645680.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fast gradient high performance liquid chromatography method with UV detection for simultaneous determination of seven angiotensin converting enzyme inhibitors together with hydrochlorothiazide in pharmaceutical dosage forms and spiked human plasma and urine. AU - Elsebaei,Fawzi, AU - Zhu,Yan, Y1 - 2011/03/30/ PY - 2010/12/23/received PY - 2011/03/09/revised PY - 2011/03/17/accepted PY - 2011/6/8/entrez PY - 2011/6/8/pubmed PY - 2011/10/1/medline SP - 123 EP - 9 JF - Talanta JO - Talanta VL - 85 IS - 1 N2 - The development of a reversed phase liquid chromatographic method for the simultaneous determination of seven angiotensin converting enzyme (ACE) inhibitors; five drugs namely benazepril HCl (BZL), enalapril maleate (ENL), fosinopril sodium (FSP), lisinopril (LSP) and ramipril (RMP) and two metabolites captopril disulfide (CPD) and enalaprilat (ENT) together with hydrochlorothiazide (HCT) is described. The method can serve as a substitute for many published papers for the analysis of the targeted compounds with or without hydrochloothiazide in pharmaceutical formulations as well as in spiked human plasma and urine samples. The method utilizes a simple gradient procedure for the separation in a 11 min run time using acetonitrile aqueous ammonia buffer (pH 9) solution and an Extend RP-C18 (25 μm particle size, 4.6 mm×250 mm, Agilent) HPLC column. The effluent was monitored on a UV detector at 215 nm. The effect of pH, solvent strength and analysis time on the peak shape and quantification were carefully studied in order to optimize the method. Adopting the proposed procedure, the analytes produce well-shaped peaks with good linear relationship over the investigated concentration ranges. The limits of detection (LOD) and limits of quantification (LOQ) from standard drug solutions lie in the range of 17-64 and 56-212 ng mL(-1), respectively. Correlation coefficient values (r) higher than 0.997 were obtained for all the studied drugs in spiked human plasma and urine samples. The intra-day and inter-day precision of the method was evaluated with relative standard deviation values being satisfactory for their purposed analysis. The method was validated with respect to specificity, recovery, accuracy, precision and linearity. SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/21645680/Fast_gradient_high_performance_liquid_chromatography_method_with_UV_detection_for_simultaneous_determination_of_seven_angiotensin_converting_enzyme_inhibitors_together_with_hydrochlorothiazide_in_pharmaceutical_dosage_forms_and_spiked_human_plasma_and_urine_ DB - PRIME DP - Unbound Medicine ER -