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Single solid phase extraction method for the simultaneous analysis of polar and non-polar pesticides in urine samples by gas chromatography and ultra high pressure liquid chromatography coupled to tandem mass spectrometry.
Talanta. 2011 Jul 15; 85(1):183-96.T

Abstract

A new multiresidue method has been developed and validated for the simultaneous extraction of more than two hundred pesticides, including non-polar and polar pesticides (carbamates, organochlorine, organophosphorous, pyrethroids, herbicides and insecticides) in urine at trace levels by gas and ultra high pressure liquid chromatography coupled to ion trap and triple quadrupole mass spectrometry, respectively (GC-IT-MS/MS, UHPLC-QqQ-MS/MS). Non-polar and polar pesticides were simultaneously extracted from urine samples by a simple and fast solid phase extraction (SPE) procedure using C(18) cartridges as sorbent, and dichloromethane as elution solvent. Recovery was in the range of 60-120%. Precision values expressed as relative standard deviation (RSD) were lower than 25%. Identification and confirmation of the compounds were performed by the use of retention time windows, comparison of spectra (GC-amenable compounds) or the estimation of the ion ratio (LC-amenable compounds). For GC-amenable pesticides, limits of detection (LODs) ranged from 0.001 to 0.436 μg L(-1) and limits of quantification (LOQs) from 0.003 to 1.452 μg L(-1). For LC-amenable pesticides, LODs ranged from 0.003 to 1.048 μg L(-1) and LOQs ranged from 0.011 to 3.494 μg L(-1). Finally, the optimized method was applied to the analysis of fourteen real samples of infants from agricultural population. Some pesticides such as methoxyfenozide, tebufenozide, piperonyl butoxide and propoxur were found at concentrations ranged from 1.61 to 24.4 μg L(-1), whereas methiocarb sulfoxide was detected at trace levels in two samples.

Authors+Show Affiliations

Group "Analytical Chemistry of Contaminants", Department of Analytical Chemistry, Almeria University, E-04071 Almeria, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21645688

Citation

Cazorla-Reyes, Rocío, et al. "Single Solid Phase Extraction Method for the Simultaneous Analysis of Polar and Non-polar Pesticides in Urine Samples By Gas Chromatography and Ultra High Pressure Liquid Chromatography Coupled to Tandem Mass Spectrometry." Talanta, vol. 85, no. 1, 2011, pp. 183-96.
Cazorla-Reyes R, Fernández-Moreno JL, Romero-González R, et al. Single solid phase extraction method for the simultaneous analysis of polar and non-polar pesticides in urine samples by gas chromatography and ultra high pressure liquid chromatography coupled to tandem mass spectrometry. Talanta. 2011;85(1):183-96.
Cazorla-Reyes, R., Fernández-Moreno, J. L., Romero-González, R., Frenich, A. G., & Vidal, J. L. (2011). Single solid phase extraction method for the simultaneous analysis of polar and non-polar pesticides in urine samples by gas chromatography and ultra high pressure liquid chromatography coupled to tandem mass spectrometry. Talanta, 85(1), 183-96. https://doi.org/10.1016/j.talanta.2011.03.048
Cazorla-Reyes R, et al. Single Solid Phase Extraction Method for the Simultaneous Analysis of Polar and Non-polar Pesticides in Urine Samples By Gas Chromatography and Ultra High Pressure Liquid Chromatography Coupled to Tandem Mass Spectrometry. Talanta. 2011 Jul 15;85(1):183-96. PubMed PMID: 21645688.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Single solid phase extraction method for the simultaneous analysis of polar and non-polar pesticides in urine samples by gas chromatography and ultra high pressure liquid chromatography coupled to tandem mass spectrometry. AU - Cazorla-Reyes,Rocío, AU - Fernández-Moreno,José Luis, AU - Romero-González,Roberto, AU - Frenich,Antonia Garrido, AU - Vidal,José Luis Martínez, Y1 - 2011/03/31/ PY - 2010/11/02/received PY - 2011/03/08/revised PY - 2011/03/20/accepted PY - 2011/6/8/entrez PY - 2011/6/8/pubmed PY - 2011/10/1/medline SP - 183 EP - 96 JF - Talanta JO - Talanta VL - 85 IS - 1 N2 - A new multiresidue method has been developed and validated for the simultaneous extraction of more than two hundred pesticides, including non-polar and polar pesticides (carbamates, organochlorine, organophosphorous, pyrethroids, herbicides and insecticides) in urine at trace levels by gas and ultra high pressure liquid chromatography coupled to ion trap and triple quadrupole mass spectrometry, respectively (GC-IT-MS/MS, UHPLC-QqQ-MS/MS). Non-polar and polar pesticides were simultaneously extracted from urine samples by a simple and fast solid phase extraction (SPE) procedure using C(18) cartridges as sorbent, and dichloromethane as elution solvent. Recovery was in the range of 60-120%. Precision values expressed as relative standard deviation (RSD) were lower than 25%. Identification and confirmation of the compounds were performed by the use of retention time windows, comparison of spectra (GC-amenable compounds) or the estimation of the ion ratio (LC-amenable compounds). For GC-amenable pesticides, limits of detection (LODs) ranged from 0.001 to 0.436 μg L(-1) and limits of quantification (LOQs) from 0.003 to 1.452 μg L(-1). For LC-amenable pesticides, LODs ranged from 0.003 to 1.048 μg L(-1) and LOQs ranged from 0.011 to 3.494 μg L(-1). Finally, the optimized method was applied to the analysis of fourteen real samples of infants from agricultural population. Some pesticides such as methoxyfenozide, tebufenozide, piperonyl butoxide and propoxur were found at concentrations ranged from 1.61 to 24.4 μg L(-1), whereas methiocarb sulfoxide was detected at trace levels in two samples. SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/21645688/Single_solid_phase_extraction_method_for_the_simultaneous_analysis_of_polar_and_non_polar_pesticides_in_urine_samples_by_gas_chromatography_and_ultra_high_pressure_liquid_chromatography_coupled_to_tandem_mass_spectrometry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0039-9140(11)00253-0 DB - PRIME DP - Unbound Medicine ER -