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Peripheral blood mononuclear cells from patients with bronchial asthma show impaired innate immune responses to rhinovirus in vitro.
Int Arch Allergy Immunol 2011; 155 Suppl 1:27-33IA

Abstract

BACKGROUND

Asthmatic patients have a higher susceptibility to rhinovirus (RV) infection, and impaired IFN-β and IFN-λ production has been demonstrated in bronchial epithelial cells from asthmatic adults upon exposure to RV. However, the mechanisms underlying the increased susceptibility of asthmatic patients to RV infection remain poorly understood. The present study aimed to elucidate the characteristics of the immune responses of asthmatic patients' peripheral blood mononuclear cells (PBMCs) to RV exposure.

METHODS

PBMCs obtained from 3 different age groups (2-6 years: young-children group; 7-19 years: youth group; ≥20 years: adult group) of asthmatic patients and nonasthmatic control subjects were stimulated with RV-14 for 72 h. Healthy adults with a history of childhood asthma were also enrolled. The concentrations of IFN-α, IL-6, TNF-α, IL-10, and soluble Fas ligand (sFasL) in the culture supernatants were measured by ELISA.

RESULTS

When compared with age-matched control subjects, IFN-α production was significantly lower in the asthmatic youth group. IL-6, TNF-α, IL-10, and sFasL productions were significantly lower in both the asthmatic youth group and the adult group. Such impaired responses were not found in healthy adults with a history of childhood asthma. No significantly different responses were found between the asthmatics and controls in the young-children group, whereas young asthmatic children with persistent wheeze during a 2-year follow-up showed significantly lower IL-10 production than those without wheeze.

CONCLUSIONS

These results imply the involvement of impaired production of both IFN-α and inflammatory cytokines seen in asthmatic patients' PBMCs upon exposure to RV in the higher susceptibility of those patients to RV infection.

Authors+Show Affiliations

Department of Pediatrics, Jikei University School of Medicine, Tokyo, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21646792

Citation

Iikura, Katsuhito, et al. "Peripheral Blood Mononuclear Cells From Patients With Bronchial Asthma Show Impaired Innate Immune Responses to Rhinovirus in Vitro." International Archives of Allergy and Immunology, vol. 155 Suppl 1, 2011, pp. 27-33.
Iikura K, Katsunuma T, Saika S, et al. Peripheral blood mononuclear cells from patients with bronchial asthma show impaired innate immune responses to rhinovirus in vitro. Int Arch Allergy Immunol. 2011;155 Suppl 1:27-33.
Iikura, K., Katsunuma, T., Saika, S., Saito, S., Ichinohe, S., Ida, H., ... Matsumoto, K. (2011). Peripheral blood mononuclear cells from patients with bronchial asthma show impaired innate immune responses to rhinovirus in vitro. International Archives of Allergy and Immunology, 155 Suppl 1, pp. 27-33. doi:10.1159/000327262.
Iikura K, et al. Peripheral Blood Mononuclear Cells From Patients With Bronchial Asthma Show Impaired Innate Immune Responses to Rhinovirus in Vitro. Int Arch Allergy Immunol. 2011;155 Suppl 1:27-33. PubMed PMID: 21646792.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Peripheral blood mononuclear cells from patients with bronchial asthma show impaired innate immune responses to rhinovirus in vitro. AU - Iikura,Katsuhito, AU - Katsunuma,Toshio, AU - Saika,Shizuko, AU - Saito,Saburo, AU - Ichinohe,Sadato, AU - Ida,Hiroyuki, AU - Saito,Hirohisa, AU - Matsumoto,Kenji, Y1 - 2011/06/01/ PY - 2011/6/8/entrez PY - 2011/6/10/pubmed PY - 2011/7/14/medline SP - 27 EP - 33 JF - International archives of allergy and immunology JO - Int. Arch. Allergy Immunol. VL - 155 Suppl 1 N2 - BACKGROUND: Asthmatic patients have a higher susceptibility to rhinovirus (RV) infection, and impaired IFN-β and IFN-λ production has been demonstrated in bronchial epithelial cells from asthmatic adults upon exposure to RV. However, the mechanisms underlying the increased susceptibility of asthmatic patients to RV infection remain poorly understood. The present study aimed to elucidate the characteristics of the immune responses of asthmatic patients' peripheral blood mononuclear cells (PBMCs) to RV exposure. METHODS: PBMCs obtained from 3 different age groups (2-6 years: young-children group; 7-19 years: youth group; ≥20 years: adult group) of asthmatic patients and nonasthmatic control subjects were stimulated with RV-14 for 72 h. Healthy adults with a history of childhood asthma were also enrolled. The concentrations of IFN-α, IL-6, TNF-α, IL-10, and soluble Fas ligand (sFasL) in the culture supernatants were measured by ELISA. RESULTS: When compared with age-matched control subjects, IFN-α production was significantly lower in the asthmatic youth group. IL-6, TNF-α, IL-10, and sFasL productions were significantly lower in both the asthmatic youth group and the adult group. Such impaired responses were not found in healthy adults with a history of childhood asthma. No significantly different responses were found between the asthmatics and controls in the young-children group, whereas young asthmatic children with persistent wheeze during a 2-year follow-up showed significantly lower IL-10 production than those without wheeze. CONCLUSIONS: These results imply the involvement of impaired production of both IFN-α and inflammatory cytokines seen in asthmatic patients' PBMCs upon exposure to RV in the higher susceptibility of those patients to RV infection. SN - 1423-0097 UR - https://www.unboundmedicine.com/medline/citation/21646792/Peripheral_blood_mononuclear_cells_from_patients_with_bronchial_asthma_show_impaired_innate_immune_responses_to_rhinovirus_in_vitro_ L2 - https://www.karger.com?DOI=10.1159/000327262 DB - PRIME DP - Unbound Medicine ER -