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Involvement of NADPH oxidase in up-regulation of plasminogen activator inhibitor-1 and heat shock factor-1 in mouse embryo fibroblasts induced by oxidized LDL and in apolipoprotein E-deficient mice.
Free Radic Res. 2011 Sep; 45(9):1013-23.FR

Abstract

The present study demonstrated that oxidized LDL (oLDL) increased the generation of superoxide and hydrogen peroxide (H(2)O(2)), the abundances of NADPH oxidase (NOX)4, NOX2, p22-phox and lectin-like oLDL receptor-1 (LOX-1) in wild-type or heat shock factor-1 (HSF1)-deficient mouse embryo fibroblasts (MEF). LOX-1 antibody inhibited LDL or oLDL-induced expression of NOX components in MEF. Abundance of HSF1 or plasminogen activator inhibitor-1 (PAI-1) was increased by oLDL in wild-type, but not in HSF1-deficient MEF. Diphenyleneiodonium or siRNA for NOX or p22-phox inhibited oLDL-induced increases of HSF1, PAI-1 and H(2)O(2) in MEF. Increased NOX4, NOX2, LOX1, HSF1 and PAI-1 were detected in aortae and hearts of apolipoprotein E-knockout (apoE-KO) mice compared to controls, which were associated with increased serum cholesterol or plasma PAI-1. The results suggest that NOX is required for oLDL-induced HSF1 or PAI-1 expression in MEF, which was supported by the up-regulation of NOX, LOX-1, HSF1 and PAI-1 in apoE-KO mice.

Authors+Show Affiliations

Department of Internal Medicine, University of Manitoba, Winnipeg, MB, Canada.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21651452

Citation

Zhao, Ruozhi, et al. "Involvement of NADPH Oxidase in Up-regulation of Plasminogen Activator Inhibitor-1 and Heat Shock Factor-1 in Mouse Embryo Fibroblasts Induced By Oxidized LDL and in Apolipoprotein E-deficient Mice." Free Radical Research, vol. 45, no. 9, 2011, pp. 1013-23.
Zhao R, Moghadasian MH, Shen GX. Involvement of NADPH oxidase in up-regulation of plasminogen activator inhibitor-1 and heat shock factor-1 in mouse embryo fibroblasts induced by oxidized LDL and in apolipoprotein E-deficient mice. Free Radic Res. 2011;45(9):1013-23.
Zhao, R., Moghadasian, M. H., & Shen, G. X. (2011). Involvement of NADPH oxidase in up-regulation of plasminogen activator inhibitor-1 and heat shock factor-1 in mouse embryo fibroblasts induced by oxidized LDL and in apolipoprotein E-deficient mice. Free Radical Research, 45(9), 1013-23. https://doi.org/10.3109/10715762.2011.587419
Zhao R, Moghadasian MH, Shen GX. Involvement of NADPH Oxidase in Up-regulation of Plasminogen Activator Inhibitor-1 and Heat Shock Factor-1 in Mouse Embryo Fibroblasts Induced By Oxidized LDL and in Apolipoprotein E-deficient Mice. Free Radic Res. 2011;45(9):1013-23. PubMed PMID: 21651452.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Involvement of NADPH oxidase in up-regulation of plasminogen activator inhibitor-1 and heat shock factor-1 in mouse embryo fibroblasts induced by oxidized LDL and in apolipoprotein E-deficient mice. AU - Zhao,Ruozhi, AU - Moghadasian,Mohammed H, AU - Shen,Garry X, Y1 - 2011/06/09/ PY - 2011/6/10/entrez PY - 2011/6/10/pubmed PY - 2011/12/13/medline SP - 1013 EP - 23 JF - Free radical research JO - Free Radic Res VL - 45 IS - 9 N2 - The present study demonstrated that oxidized LDL (oLDL) increased the generation of superoxide and hydrogen peroxide (H(2)O(2)), the abundances of NADPH oxidase (NOX)4, NOX2, p22-phox and lectin-like oLDL receptor-1 (LOX-1) in wild-type or heat shock factor-1 (HSF1)-deficient mouse embryo fibroblasts (MEF). LOX-1 antibody inhibited LDL or oLDL-induced expression of NOX components in MEF. Abundance of HSF1 or plasminogen activator inhibitor-1 (PAI-1) was increased by oLDL in wild-type, but not in HSF1-deficient MEF. Diphenyleneiodonium or siRNA for NOX or p22-phox inhibited oLDL-induced increases of HSF1, PAI-1 and H(2)O(2) in MEF. Increased NOX4, NOX2, LOX1, HSF1 and PAI-1 were detected in aortae and hearts of apolipoprotein E-knockout (apoE-KO) mice compared to controls, which were associated with increased serum cholesterol or plasma PAI-1. The results suggest that NOX is required for oLDL-induced HSF1 or PAI-1 expression in MEF, which was supported by the up-regulation of NOX, LOX-1, HSF1 and PAI-1 in apoE-KO mice. SN - 1029-2470 UR - https://www.unboundmedicine.com/medline/citation/21651452/Involvement_of_NADPH_oxidase_in_up_regulation_of_plasminogen_activator_inhibitor_1_and_heat_shock_factor_1_in_mouse_embryo_fibroblasts_induced_by_oxidized_LDL_and_in_apolipoprotein_E_deficient_mice_ L2 - https://www.tandfonline.com/doi/full/10.3109/10715762.2011.587419 DB - PRIME DP - Unbound Medicine ER -