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RETRACTED ARTICLE

Liver conversion of docosahexaenoic and arachidonic acids from their 18-carbon precursors in rats on a DHA-free but α-LNA-containing n-3 PUFA adequate diet.
Biochim Biophys Acta. 2011 Jul-Aug; 1811(7-8):484-9.BB

Abstract

The long-chain polyunsaturated fatty acids (PUFAs), eicosapentaenoic acid (EPA, 20:5n-3), docosahexaenoic acid (DHA, 22:6n-3), and arachidonic acid (AA, 20:4n-6), are critical for health. These PUFAs can be synthesized in liver from their plant-derived precursors, α-linolenic acid (α-LNA, 18:3n-3) and linoleic acid (LA, 18:2n-6). Vegetarians and vegans may have suboptimal long-chain n-3 PUFA status, and the extent of the conversion of α-LNA to EPA and DHA by the liver is debatable. We quantified liver conversion of DHA and other n-3 PUFAs from α-LNA in rats fed a DHA-free but α-LNA (n-3 PUFA) adequate diet, and compared results to conversion of LA to AA. [U-(13)C]LA or [U-(13)C]α-LNA was infused intravenously for 2h at a constant rate into unanesthetized rats fed a DHA-free α-LNA adequate diet, and published equations were used to calculate kinetic parameters. The conversion coefficient k(⁎) of DHA from α-LNA was much higher than for AA from LA (97.2×10(-3) vs. 10.6×10(-3)min(-1)), suggesting that liver elongation-desaturation is more selective for n-3 PUFA biosynthesis on a per molecule basis. The net daily secretion rate of DHA, 20.3μmol/day, exceeded the reported brain DHA consumption rate by 50-fold, suggesting that the liver can maintain brain DHA metabolism with an adequate dietary supply solely of α-LNA. This infusion method could be used in vegetarians or vegans to determine minimal daily requirements of EPA and DHA in humans.

Authors+Show Affiliations

National Institute on Aging, National Institutes of Health, Bethesda, MD 20892, USA. gaof@mail.nih.govNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Intramural
Retracted Publication

Language

eng

PubMed ID

21651989

Citation

Gao, Fei, et al. "Liver Conversion of Docosahexaenoic and Arachidonic Acids From Their 18-carbon Precursors in Rats On a DHA-free but α-LNA-containing N-3 PUFA Adequate Diet." Biochimica Et Biophysica Acta, vol. 1811, no. 7-8, 2011, pp. 484-9.
Gao F, Kim HW, Igarashi M, et al. Liver conversion of docosahexaenoic and arachidonic acids from their 18-carbon precursors in rats on a DHA-free but α-LNA-containing n-3 PUFA adequate diet. Biochim Biophys Acta. 2011;1811(7-8):484-9.
Gao, F., Kim, H. W., Igarashi, M., Kiesewetter, D., Chang, L., Ma, K., & Rapoport, S. I. (2011). Liver conversion of docosahexaenoic and arachidonic acids from their 18-carbon precursors in rats on a DHA-free but α-LNA-containing n-3 PUFA adequate diet. Biochimica Et Biophysica Acta, 1811(7-8), 484-9. https://doi.org/10.1016/j.bbalip.2011.05.008
Gao F, et al. Liver Conversion of Docosahexaenoic and Arachidonic Acids From Their 18-carbon Precursors in Rats On a DHA-free but α-LNA-containing N-3 PUFA Adequate Diet. Biochim Biophys Acta. 2011 Jul-Aug;1811(7-8):484-9. PubMed PMID: 21651989.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Liver conversion of docosahexaenoic and arachidonic acids from their 18-carbon precursors in rats on a DHA-free but α-LNA-containing n-3 PUFA adequate diet. AU - Gao,Fei, AU - Kim,Hyung-Wook, AU - Igarashi,Miki, AU - Kiesewetter,Dale, AU - Chang,Lisa, AU - Ma,Kaizong, AU - Rapoport,Stanley I, Y1 - 2011/05/30/ PY - 2011/03/17/received PY - 2011/05/05/revised PY - 2011/05/23/accepted PY - 2011/6/10/entrez PY - 2011/6/10/pubmed PY - 2011/8/31/medline SP - 484 EP - 9 JF - Biochimica et biophysica acta JO - Biochim Biophys Acta VL - 1811 IS - 7-8 N2 - The long-chain polyunsaturated fatty acids (PUFAs), eicosapentaenoic acid (EPA, 20:5n-3), docosahexaenoic acid (DHA, 22:6n-3), and arachidonic acid (AA, 20:4n-6), are critical for health. These PUFAs can be synthesized in liver from their plant-derived precursors, α-linolenic acid (α-LNA, 18:3n-3) and linoleic acid (LA, 18:2n-6). Vegetarians and vegans may have suboptimal long-chain n-3 PUFA status, and the extent of the conversion of α-LNA to EPA and DHA by the liver is debatable. We quantified liver conversion of DHA and other n-3 PUFAs from α-LNA in rats fed a DHA-free but α-LNA (n-3 PUFA) adequate diet, and compared results to conversion of LA to AA. [U-(13)C]LA or [U-(13)C]α-LNA was infused intravenously for 2h at a constant rate into unanesthetized rats fed a DHA-free α-LNA adequate diet, and published equations were used to calculate kinetic parameters. The conversion coefficient k(⁎) of DHA from α-LNA was much higher than for AA from LA (97.2×10(-3) vs. 10.6×10(-3)min(-1)), suggesting that liver elongation-desaturation is more selective for n-3 PUFA biosynthesis on a per molecule basis. The net daily secretion rate of DHA, 20.3μmol/day, exceeded the reported brain DHA consumption rate by 50-fold, suggesting that the liver can maintain brain DHA metabolism with an adequate dietary supply solely of α-LNA. This infusion method could be used in vegetarians or vegans to determine minimal daily requirements of EPA and DHA in humans. SN - 0006-3002 UR - https://www.unboundmedicine.com/medline/citation/21651989/Liver_conversion_of_docosahexaenoic_and_arachidonic_acids_from_their_18_carbon_precursors_in_rats_on_a_DHA_free_but_α_LNA_containing_n_3_PUFA_adequate_diet_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1388-1981(11)00075-8 DB - PRIME DP - Unbound Medicine ER -