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Distribution of isoflavones in samples of serum, liver and mammary glands of rats or pigs fed dietary isoflavones.

Abstract

BACKGROUND/AIMS

There has been great interest in the potential beneficial and adverse health effects of dietary isoflavones. Determination of tissue concentrations of isoflavone metabolites provides an insight into the potential bioactivity of dietary isoflavones. However, data on the distribution of isoflavones in animal models fed dietary isoflavones are limited. In this study, additional data on the distribution of isoflavones in serum and/or tissues of rats and pigs fed dietary isoflavones were generated.

METHODS

Rats (male and female) were fed a casein control diet (containing no isoflavones) and an isoflavone-supplemented diet (containing an alcohol-washed soy protein isolate plus NOVASOY, providing a total of 1,047 mg/kg of total isoflavones). Female pigs were fed a control diet (without soy) containing 17.5 mg/kg of isoflavones, a soy diet containing 582.8 mg/kg of isoflavones or a soy diet supplemented with a daily dose of 2.3 g (equivalent to 42.0 and 14.5 mg/kg of body weight at the onset and end of treatment, respectively) of crystalline genistein. The concentrations of isoflavones in serum and tissues (liver and mammary gland) and in tissues (liver and mammary gland) of pigs were determined via a sensitive and rapid method using liquid chromatography/mass spectrometry.

RESULTS

Rats fed the control diet containing no isoflavones had nondetectable levels of isoflavone metabolites in serum, liver and mammary gland samples. Rats fed the isoflavone-supplemented diet had the greatest levels of equol, followed by genistein, daidzein and glycitein, respectively, in their serum, livers and mammary glands. The concentrations of total isoflavones (daidzein, equol and genistein plus glycitein) in serum were significantly (p < 0.05) greater in male rats vs. female rats, but the reverse was true in the case of livers. Concentrations of daidzein, equol, genistein and glycitein were lowest (p < 0.05) in the livers of pigs fed the control diet, and in the mammary glands of female pigs there was only an effect of feeding soy plus genistein on the concentrations of daidzein and equol (p <0.05).

CONCLUSIONS

The current data therefore show gender as well as species differences in the tissue distribution of isoflavones.

Links

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  • Authors+Show Affiliations

    ,

    Nutrition Research Division, Health Canada, Ottawa, Ont., Canada. Sarwar.Gilani@hc-sc.gc.ca

    , , , , , , , ,

    Source

    MeSH

    Animals
    Body Weight
    Chromatography, Liquid
    Diet
    Dietary Supplements
    Equol
    Female
    Genistein
    Isoflavones
    Liver
    Male
    Mammary Glands, Animal
    Mass Spectrometry
    Rats
    Rats, Sprague-Dawley
    Soybean Proteins
    Swine

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    21654164

    Citation

    Gilani, G Sarwar, et al. "Distribution of Isoflavones in Samples of Serum, Liver and Mammary Glands of Rats or Pigs Fed Dietary Isoflavones." Annals of Nutrition & Metabolism, vol. 58, no. 3, 2011, pp. 171-80.
    Gilani GS, Farmer C, Dyck M, et al. Distribution of isoflavones in samples of serum, liver and mammary glands of rats or pigs fed dietary isoflavones. Ann Nutr Metab. 2011;58(3):171-80.
    Gilani, G. S., Farmer, C., Dyck, M., Robertson, P., Dahiya, J., Sepehr, E., ... Cooke, G. M. (2011). Distribution of isoflavones in samples of serum, liver and mammary glands of rats or pigs fed dietary isoflavones. Annals of Nutrition & Metabolism, 58(3), pp. 171-80. doi:10.1159/000328771.
    Gilani GS, et al. Distribution of Isoflavones in Samples of Serum, Liver and Mammary Glands of Rats or Pigs Fed Dietary Isoflavones. Ann Nutr Metab. 2011;58(3):171-80. PubMed PMID: 21654164.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Distribution of isoflavones in samples of serum, liver and mammary glands of rats or pigs fed dietary isoflavones. AU - Gilani,G Sarwar, AU - Farmer,Chantal, AU - Dyck,Monica, AU - Robertson,Patrick, AU - Dahiya,Jagroop, AU - Sepehr,Estatira, AU - Fan,Li, AU - Nicolidakis,Helen, AU - Curran,Ivan, AU - Cooke,Gerard M, Y1 - 2011/06/08/ PY - 2010/07/19/received PY - 2011/04/10/accepted PY - 2011/6/10/entrez PY - 2011/6/10/pubmed PY - 2011/12/23/medline SP - 171 EP - 80 JF - Annals of nutrition & metabolism JO - Ann. Nutr. Metab. VL - 58 IS - 3 N2 - BACKGROUND/AIMS: There has been great interest in the potential beneficial and adverse health effects of dietary isoflavones. Determination of tissue concentrations of isoflavone metabolites provides an insight into the potential bioactivity of dietary isoflavones. However, data on the distribution of isoflavones in animal models fed dietary isoflavones are limited. In this study, additional data on the distribution of isoflavones in serum and/or tissues of rats and pigs fed dietary isoflavones were generated. METHODS: Rats (male and female) were fed a casein control diet (containing no isoflavones) and an isoflavone-supplemented diet (containing an alcohol-washed soy protein isolate plus NOVASOY, providing a total of 1,047 mg/kg of total isoflavones). Female pigs were fed a control diet (without soy) containing 17.5 mg/kg of isoflavones, a soy diet containing 582.8 mg/kg of isoflavones or a soy diet supplemented with a daily dose of 2.3 g (equivalent to 42.0 and 14.5 mg/kg of body weight at the onset and end of treatment, respectively) of crystalline genistein. The concentrations of isoflavones in serum and tissues (liver and mammary gland) and in tissues (liver and mammary gland) of pigs were determined via a sensitive and rapid method using liquid chromatography/mass spectrometry. RESULTS: Rats fed the control diet containing no isoflavones had nondetectable levels of isoflavone metabolites in serum, liver and mammary gland samples. Rats fed the isoflavone-supplemented diet had the greatest levels of equol, followed by genistein, daidzein and glycitein, respectively, in their serum, livers and mammary glands. The concentrations of total isoflavones (daidzein, equol and genistein plus glycitein) in serum were significantly (p < 0.05) greater in male rats vs. female rats, but the reverse was true in the case of livers. Concentrations of daidzein, equol, genistein and glycitein were lowest (p < 0.05) in the livers of pigs fed the control diet, and in the mammary glands of female pigs there was only an effect of feeding soy plus genistein on the concentrations of daidzein and equol (p <0.05). CONCLUSIONS: The current data therefore show gender as well as species differences in the tissue distribution of isoflavones. SN - 1421-9697 UR - https://www.unboundmedicine.com/medline/citation/21654164/Distribution_of_isoflavones_in_samples_of_serum_liver_and_mammary_glands_of_rats_or_pigs_fed_dietary_isoflavones_ L2 - https://www.karger.com?DOI=10.1159/000328771 DB - PRIME DP - Unbound Medicine ER -