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[Simultaneous determination of six flavonoids in Apocynum venetum by HPLC].
Zhongguo Zhong Yao Za Zhi. 2011 Mar; 36(5):589-93.ZZ

Abstract

OBJECTIVE

To develop a high-performance liquid chromatography (HPLC) method for the simultaneous determination of rutin, hyperoside, isoquercetin, astragulin, quercetin, and kaempferol in Apocynum venetum and its extracts.

METHOD

The separation was carried out on a Shim pack ODS (4.6 mm x 250 mm, 5 microm) colum eluted with in mobile phases of water containing 0.2% phosphoric acid and acetonitrile containing 0.2% phosphoric acid in acetonitrile gradient mode. The column temperature was 40 degrees C, and the flow rate was 1.0 mL x min(-1). The detection wavelength was set at 360 nm.

RESULT

The good seperation of six flavonoids was achieved within 40 min, with the relative standard deviations (RSD) of intra- and inter-day precision < or = 2.0%. Calibration curves of rutin, hyperoside, isoquercetin, astragulin, quercetin, and kaempferol showed good linear relationship (R2 > 0.999 7, n = 6). The average recoveries of the six flavonoids were within 97.30% - 105.8% (RSD 2.6%). Three batches of A. venetum and 2 batches of its extracts were determined.

CONCLUSION

The developed method is simple, accurate, and repeatable, and can be readily used as a powerful tool for the quality control of A. venetum and its extracts.

Authors+Show Affiliations

Key Anhui Provincial key Lab of Natural Medicine Active Research, College of Pharmacy, Anhui Medical University, Hefei 230032, China. qunlinehang@163.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

21657078

Citation

Zhang, Qunlin, et al. "[Simultaneous Determination of Six Flavonoids in Apocynum Venetum By HPLC]." Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, vol. 36, no. 5, 2011, pp. 589-93.
Zhang Q, Wu L, Yan A, et al. [Simultaneous determination of six flavonoids in Apocynum venetum by HPLC]. Zhongguo Zhong Yao Za Zhi. 2011;36(5):589-93.
Zhang, Q., Wu, L., Yan, A., Hu, F., Yuan, Y., & Wang, J. (2011). [Simultaneous determination of six flavonoids in Apocynum venetum by HPLC]. Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, 36(5), 589-93.
Zhang Q, et al. [Simultaneous Determination of Six Flavonoids in Apocynum Venetum By HPLC]. Zhongguo Zhong Yao Za Zhi. 2011;36(5):589-93. PubMed PMID: 21657078.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Simultaneous determination of six flavonoids in Apocynum venetum by HPLC]. AU - Zhang,Qunlin, AU - Wu,Liang, AU - Yan,Anding, AU - Hu,Feiyan, AU - Yuan,Ye, AU - Wang,Juncheng, PY - 2011/6/11/entrez PY - 2011/6/11/pubmed PY - 2011/7/9/medline SP - 589 EP - 93 JF - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica JO - Zhongguo Zhong Yao Za Zhi VL - 36 IS - 5 N2 - OBJECTIVE: To develop a high-performance liquid chromatography (HPLC) method for the simultaneous determination of rutin, hyperoside, isoquercetin, astragulin, quercetin, and kaempferol in Apocynum venetum and its extracts. METHOD: The separation was carried out on a Shim pack ODS (4.6 mm x 250 mm, 5 microm) colum eluted with in mobile phases of water containing 0.2% phosphoric acid and acetonitrile containing 0.2% phosphoric acid in acetonitrile gradient mode. The column temperature was 40 degrees C, and the flow rate was 1.0 mL x min(-1). The detection wavelength was set at 360 nm. RESULT: The good seperation of six flavonoids was achieved within 40 min, with the relative standard deviations (RSD) of intra- and inter-day precision < or = 2.0%. Calibration curves of rutin, hyperoside, isoquercetin, astragulin, quercetin, and kaempferol showed good linear relationship (R2 > 0.999 7, n = 6). The average recoveries of the six flavonoids were within 97.30% - 105.8% (RSD 2.6%). Three batches of A. venetum and 2 batches of its extracts were determined. CONCLUSION: The developed method is simple, accurate, and repeatable, and can be readily used as a powerful tool for the quality control of A. venetum and its extracts. SN - 1001-5302 UR - https://www.unboundmedicine.com/medline/citation/21657078/[Simultaneous_determination_of_six_flavonoids_in_Apocynum_venetum_by_HPLC]_ L2 - https://antibodies.cancer.gov/detail/CPTC-RRM2-2 DB - PRIME DP - Unbound Medicine ER -