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Investigating the barrier function of skin lipid models with varying compositions.
Eur J Pharm Biopharm. 2011 Oct; 79(2):334-42.EJ

Abstract

The lipids in the uppermost layer of the skin, the stratum corneum (SC), play an important role in the barrier function. The main lipid classes in stratum corneum are ceramides, cholesterol, and free fatty acids. In previous publications, a lipid model was presented, referred to as the stratum corneum substitute (SCS), that closely mimics the SC lipid organization and SC barrier function. In the present study, we use the SCS to study the effect of changes in lipid organization on the lipid barrier function using benzoic acid as permeation compound. First, in the SCS, we increased the level of one of the three major lipid classes keeping the ratio between the other lipid classes constant. An increased cholesterol level resulted in an increase in phase-separated cholesterol and a reduction in the permeability. An increase in ceramide or free fatty acid level resulted in the formation of additional phases, but had no significant influence on the permeability. We also examined models that mimic selected changes in lipid composition reported for dry or diseased skin. The SCS that mimics the composition in recessive X-linked ichthyosis skin displayed a twofold increase in permeability. This increase is possibly related to the formation of an additional, less ordered phase in this model.

Authors+Show Affiliations

Leiden/Amsterdam Center for Drug Research, Department of Drug Delivery Technology, University of Leiden, Leiden, The Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21658448

Citation

Groen, Daniël, et al. "Investigating the Barrier Function of Skin Lipid Models With Varying Compositions." European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Fur Pharmazeutische Verfahrenstechnik E.V, vol. 79, no. 2, 2011, pp. 334-42.
Groen D, Poole DS, Gooris GS, et al. Investigating the barrier function of skin lipid models with varying compositions. Eur J Pharm Biopharm. 2011;79(2):334-42.
Groen, D., Poole, D. S., Gooris, G. S., & Bouwstra, J. A. (2011). Investigating the barrier function of skin lipid models with varying compositions. European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Fur Pharmazeutische Verfahrenstechnik E.V, 79(2), 334-42. https://doi.org/10.1016/j.ejpb.2011.05.007
Groen D, et al. Investigating the Barrier Function of Skin Lipid Models With Varying Compositions. Eur J Pharm Biopharm. 2011;79(2):334-42. PubMed PMID: 21658448.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Investigating the barrier function of skin lipid models with varying compositions. AU - Groen,Daniël, AU - Poole,Dana S, AU - Gooris,Gert S, AU - Bouwstra,Joke A, Y1 - 2011/05/30/ PY - 2010/11/24/received PY - 2011/05/09/revised PY - 2011/05/23/accepted PY - 2011/6/11/entrez PY - 2011/6/11/pubmed PY - 2012/4/12/medline SP - 334 EP - 42 JF - European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V JO - Eur J Pharm Biopharm VL - 79 IS - 2 N2 - The lipids in the uppermost layer of the skin, the stratum corneum (SC), play an important role in the barrier function. The main lipid classes in stratum corneum are ceramides, cholesterol, and free fatty acids. In previous publications, a lipid model was presented, referred to as the stratum corneum substitute (SCS), that closely mimics the SC lipid organization and SC barrier function. In the present study, we use the SCS to study the effect of changes in lipid organization on the lipid barrier function using benzoic acid as permeation compound. First, in the SCS, we increased the level of one of the three major lipid classes keeping the ratio between the other lipid classes constant. An increased cholesterol level resulted in an increase in phase-separated cholesterol and a reduction in the permeability. An increase in ceramide or free fatty acid level resulted in the formation of additional phases, but had no significant influence on the permeability. We also examined models that mimic selected changes in lipid composition reported for dry or diseased skin. The SCS that mimics the composition in recessive X-linked ichthyosis skin displayed a twofold increase in permeability. This increase is possibly related to the formation of an additional, less ordered phase in this model. SN - 1873-3441 UR - https://www.unboundmedicine.com/medline/citation/21658448/Investigating_the_barrier_function_of_skin_lipid_models_with_varying_compositions_ DB - PRIME DP - Unbound Medicine ER -