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In vitro susceptibility of fluconazole-susceptible and -resistant isolates of Malassezia pachydermatis against azoles.
Vet Microbiol. 2011 Aug 26; 152(1-2):161-4.VM

Abstract

OBJECTIVES

The first aim of this study was to evaluate the in vitro efficacies of fluconazole, ketoconazole, itraconazole and voriconazole on M. pachydermatis growth inhibition. This study also evaluated M. pachydermatis azole cross-resistance, comparing wild clinical isolates and the same isolates with in vitro-induced fluconazole resistance.

METHODS

Two techniques were used: (1) a broth microdilution method based on protocol M27-A3 from the Clinical and Laboratory Standards Institute to determine the minimum inhibitory concentration (MIC) and (2) the Fekete-Forgács method to induce fluconazole resistance in vitro. The isolates were divided into two groups: group 1 included fluconazole-susceptible clinical isolates (n=30) and group 2 contained the same isolates with in vitro-induced fluconazole resistance (n=30).

RESULTS

The two groups exhibited differences in susceptibility (p<0.001). Group 1 isolates were susceptible to azoles: ketoconazole (MIC 0.01-1.0 μg/mL), itraconazole (MIC 0.01-1.0 μg/mL), voriconazole (MIC 0.01-4.0 μg/mL), and fluconazole (MIC 0.01-4.0 μg/mL). Group 2 isolates demonstrated a wider range of MICs to azoles: ITZ (MIC 0.06-64.0 μg/mL), KTZ (MIC 0.25-32.0 μg/mL), VRZ (MIC 2.0-128.0 μg/mL), and FLZ (MIC 64.0-128.0 μg/mL).

CONCLUSIONS

It was shown that FLZ-resistant M. pachydermatis isolates exhibit cross-resistance to other azoles, reinforcing the importance of susceptibility tests as a guide for the therapeutic prescription of antifungals in medical and veterinary mycology.

Authors+Show Affiliations

Programa de Pós-Graduação em Farmacologia, Universidade Federal de Santa Maria (UFSM) 97105-900, Santa Maria, RS, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21658868

Citation

Jesus, F P K., et al. "In Vitro Susceptibility of Fluconazole-susceptible and -resistant Isolates of Malassezia Pachydermatis Against Azoles." Veterinary Microbiology, vol. 152, no. 1-2, 2011, pp. 161-4.
Jesus FP, Lautert C, Zanette RA, et al. In vitro susceptibility of fluconazole-susceptible and -resistant isolates of Malassezia pachydermatis against azoles. Vet Microbiol. 2011;152(1-2):161-4.
Jesus, F. P., Lautert, C., Zanette, R. A., Mahl, D. L., Azevedo, M. I., Machado, M. L., Dutra, V., Botton, S. A., Alves, S. H., & Santurio, J. M. (2011). In vitro susceptibility of fluconazole-susceptible and -resistant isolates of Malassezia pachydermatis against azoles. Veterinary Microbiology, 152(1-2), 161-4. https://doi.org/10.1016/j.vetmic.2011.04.027
Jesus FP, et al. In Vitro Susceptibility of Fluconazole-susceptible and -resistant Isolates of Malassezia Pachydermatis Against Azoles. Vet Microbiol. 2011 Aug 26;152(1-2):161-4. PubMed PMID: 21658868.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro susceptibility of fluconazole-susceptible and -resistant isolates of Malassezia pachydermatis against azoles. AU - Jesus,F P K, AU - Lautert,C, AU - Zanette,R A, AU - Mahl,D L, AU - Azevedo,M I, AU - Machado,M L S, AU - Dutra,V, AU - Botton,S A, AU - Alves,S H, AU - Santurio,J M, Y1 - 2011/04/22/ PY - 2011/03/21/received PY - 2011/04/14/accepted PY - 2011/6/11/entrez PY - 2011/6/11/pubmed PY - 2011/10/25/medline SP - 161 EP - 4 JF - Veterinary microbiology JO - Vet. Microbiol. VL - 152 IS - 1-2 N2 - OBJECTIVES: The first aim of this study was to evaluate the in vitro efficacies of fluconazole, ketoconazole, itraconazole and voriconazole on M. pachydermatis growth inhibition. This study also evaluated M. pachydermatis azole cross-resistance, comparing wild clinical isolates and the same isolates with in vitro-induced fluconazole resistance. METHODS: Two techniques were used: (1) a broth microdilution method based on protocol M27-A3 from the Clinical and Laboratory Standards Institute to determine the minimum inhibitory concentration (MIC) and (2) the Fekete-Forgács method to induce fluconazole resistance in vitro. The isolates were divided into two groups: group 1 included fluconazole-susceptible clinical isolates (n=30) and group 2 contained the same isolates with in vitro-induced fluconazole resistance (n=30). RESULTS: The two groups exhibited differences in susceptibility (p<0.001). Group 1 isolates were susceptible to azoles: ketoconazole (MIC 0.01-1.0 μg/mL), itraconazole (MIC 0.01-1.0 μg/mL), voriconazole (MIC 0.01-4.0 μg/mL), and fluconazole (MIC 0.01-4.0 μg/mL). Group 2 isolates demonstrated a wider range of MICs to azoles: ITZ (MIC 0.06-64.0 μg/mL), KTZ (MIC 0.25-32.0 μg/mL), VRZ (MIC 2.0-128.0 μg/mL), and FLZ (MIC 64.0-128.0 μg/mL). CONCLUSIONS: It was shown that FLZ-resistant M. pachydermatis isolates exhibit cross-resistance to other azoles, reinforcing the importance of susceptibility tests as a guide for the therapeutic prescription of antifungals in medical and veterinary mycology. SN - 1873-2542 UR - https://www.unboundmedicine.com/medline/citation/21658868/In_vitro_susceptibility_of_fluconazole_susceptible_and__resistant_isolates_of_Malassezia_pachydermatis_against_azoles_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-1135(11)00241-0 DB - PRIME DP - Unbound Medicine ER -