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The inositol 5-phosphatase dOCRL controls PI(4,5)P2 homeostasis and is necessary for cytokinesis.
Curr Biol 2011; 21(12):1074-9CB

Abstract

During cytokinesis, constriction of an equatorial actomyosin ring physically separates the two daughter cells. At the cleavage furrow, the phosphoinositide PI(4,5)P2 plays an important role by recruiting and regulating essential proteins of the cytokinesis machinery [1]. Accordingly, perturbation of PI(4,5)P2 regulation leads to abortive furrowing and binucleation [2-4]. To determine how PI(4,5)P2 is regulated during cytokinesis, we individually knocked down each of the enzymes controlling the phosphoinositide (PIP) cycle in Drosophila. We show that depletion of the Drosophila ortholog of human oculocerebrorenal syndrome of Lowe 1 (OCRL1), an inositol 5-phosphatase mutated in the X-linked disorder oculocerebrorenal Lowe syndrome, triggers a high rate of cytokinesis failure. In absence of dOCRL, several essential components of the cleavage furrow were found to be incorrectly localized on giant cytoplasmic vacuoles rich in PI(4,5)P2 and in endocytic markers. We demonstrate that dOCRL is associated with endosomes and that it dephosphorylates PI(4,5)P2 on internal membranes to restrict this phosphoinositide at the plasma membrane and thereby regulates cleavage furrow formation and ingression. Identification of dOCRL as essential for cell division may be important to understand the molecular basis of the phenotypic manifestations of Lowe syndrome.

Authors+Show Affiliations

Institute for Research in Immunology and Cancer, Université de Montréal, Montreal, QC H3C 3J7, Canada.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21658948

Citation

Ben El Kadhi, Khaled, et al. "The Inositol 5-phosphatase dOCRL Controls PI(4,5)P2 Homeostasis and Is Necessary for Cytokinesis." Current Biology : CB, vol. 21, no. 12, 2011, pp. 1074-9.
Ben El Kadhi K, Roubinet C, Solinet S, et al. The inositol 5-phosphatase dOCRL controls PI(4,5)P2 homeostasis and is necessary for cytokinesis. Curr Biol. 2011;21(12):1074-9.
Ben El Kadhi, K., Roubinet, C., Solinet, S., Emery, G., & Carréno, S. (2011). The inositol 5-phosphatase dOCRL controls PI(4,5)P2 homeostasis and is necessary for cytokinesis. Current Biology : CB, 21(12), pp. 1074-9. doi:10.1016/j.cub.2011.05.030.
Ben El Kadhi K, et al. The Inositol 5-phosphatase dOCRL Controls PI(4,5)P2 Homeostasis and Is Necessary for Cytokinesis. Curr Biol. 2011 Jun 21;21(12):1074-9. PubMed PMID: 21658948.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The inositol 5-phosphatase dOCRL controls PI(4,5)P2 homeostasis and is necessary for cytokinesis. AU - Ben El Kadhi,Khaled, AU - Roubinet,Chantal, AU - Solinet,Sara, AU - Emery,Grégory, AU - Carréno,Sébastien, Y1 - 2011/06/09/ PY - 2011/01/06/received PY - 2011/04/07/revised PY - 2011/05/16/accepted PY - 2011/6/11/entrez PY - 2011/6/11/pubmed PY - 2011/10/20/medline SP - 1074 EP - 9 JF - Current biology : CB JO - Curr. Biol. VL - 21 IS - 12 N2 - During cytokinesis, constriction of an equatorial actomyosin ring physically separates the two daughter cells. At the cleavage furrow, the phosphoinositide PI(4,5)P2 plays an important role by recruiting and regulating essential proteins of the cytokinesis machinery [1]. Accordingly, perturbation of PI(4,5)P2 regulation leads to abortive furrowing and binucleation [2-4]. To determine how PI(4,5)P2 is regulated during cytokinesis, we individually knocked down each of the enzymes controlling the phosphoinositide (PIP) cycle in Drosophila. We show that depletion of the Drosophila ortholog of human oculocerebrorenal syndrome of Lowe 1 (OCRL1), an inositol 5-phosphatase mutated in the X-linked disorder oculocerebrorenal Lowe syndrome, triggers a high rate of cytokinesis failure. In absence of dOCRL, several essential components of the cleavage furrow were found to be incorrectly localized on giant cytoplasmic vacuoles rich in PI(4,5)P2 and in endocytic markers. We demonstrate that dOCRL is associated with endosomes and that it dephosphorylates PI(4,5)P2 on internal membranes to restrict this phosphoinositide at the plasma membrane and thereby regulates cleavage furrow formation and ingression. Identification of dOCRL as essential for cell division may be important to understand the molecular basis of the phenotypic manifestations of Lowe syndrome. SN - 1879-0445 UR - https://www.unboundmedicine.com/medline/citation/21658948/The_inositol_5_phosphatase_dOCRL_controls_PI_45_P2_homeostasis_and_is_necessary_for_cytokinesis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0960-9822(11)00557-4 DB - PRIME DP - Unbound Medicine ER -