Tags

Type your tag names separated by a space and hit enter

Role of alkaline phosphatase in insecticidal action of Cry1Ac against Helicoverpa armigera larvae.
Biotechnol Lett. 2011 Oct; 33(10):2027-36.BL

Abstract

Cry1Ac δ-endotoxin produced by Bacillus thuringiensis (Bt) is used as a bio-pesticide for the control of Helicoverpa armigera. Aminopeptidases N (APN) and alkaline phosphatase (ALP) play critical roles in its action against H. armigera larvae. The binding of Cry1Ac with brush border membrane vesicle (BBMV) proteins was increased with the larval development although the sensitivity of larvae to δ-endotoxins decreased. There was higher expression of ALP than APN in early instar larvae with a ~10-fold higher affinity of Cry1Ac towards ALP than to APN. Binding to a specific receptor is therefore more important for the insecticidal activity rather than overall binding to the BBMV proteins. ALP might play a major role in toxicity as compared to APN.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Upadhyay SK
National Botanical Research Institute, Council of Scientific and Industrial Research, Rana Pratap Marg, Lucknow, UP 226001, India.
Singh PK
No affiliation info available

MeSH

Alkaline PhosphataseAnimalsBacillus thuringiensis ToxinsBacterial ProteinsCD13 AntigensEndotoxinsHemolysin ProteinsInsect ProteinsInsecticidesKineticsLarvaLepidopteraMicrovilliProtein BindingSurvival Analysis

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21660568

Citation

Upadhyay, Santosh K., and Pradhyumna K. Singh. "Role of Alkaline Phosphatase in Insecticidal Action of Cry1Ac Against Helicoverpa Armigera Larvae." Biotechnology Letters, vol. 33, no. 10, 2011, pp. 2027-36.
Upadhyay SK, Singh PK. Role of alkaline phosphatase in insecticidal action of Cry1Ac against Helicoverpa armigera larvae. Biotechnol Lett. 2011;33(10):2027-36.
Upadhyay, S. K., & Singh, P. K. (2011). Role of alkaline phosphatase in insecticidal action of Cry1Ac against Helicoverpa armigera larvae. Biotechnology Letters, 33(10), 2027-36. https://doi.org/10.1007/s10529-011-0665-x
Upadhyay SK, Singh PK. Role of Alkaline Phosphatase in Insecticidal Action of Cry1Ac Against Helicoverpa Armigera Larvae. Biotechnol Lett. 2011;33(10):2027-36. PubMed PMID: 21660568.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Role of alkaline phosphatase in insecticidal action of Cry1Ac against Helicoverpa armigera larvae. AU - Upadhyay,Santosh K, AU - Singh,Pradhyumna K, Y1 - 2011/06/10/ PY - 2011/04/11/received PY - 2011/05/24/accepted PY - 2011/6/11/entrez PY - 2011/6/11/pubmed PY - 2012/2/2/medline SP - 2027 EP - 36 JF - Biotechnology letters JO - Biotechnol Lett VL - 33 IS - 10 N2 - Cry1Ac δ-endotoxin produced by Bacillus thuringiensis (Bt) is used as a bio-pesticide for the control of Helicoverpa armigera. Aminopeptidases N (APN) and alkaline phosphatase (ALP) play critical roles in its action against H. armigera larvae. The binding of Cry1Ac with brush border membrane vesicle (BBMV) proteins was increased with the larval development although the sensitivity of larvae to δ-endotoxins decreased. There was higher expression of ALP than APN in early instar larvae with a ~10-fold higher affinity of Cry1Ac towards ALP than to APN. Binding to a specific receptor is therefore more important for the insecticidal activity rather than overall binding to the BBMV proteins. ALP might play a major role in toxicity as compared to APN. SN - 1573-6776 UR - https://www.unboundmedicine.com/medline/citation/21660568/Role_of_alkaline_phosphatase_in_insecticidal_action_of_Cry1Ac_against_Helicoverpa_armigera_larvae_ L2 - https://doi.org/10.1007/s10529-011-0665-x DB - PRIME DP - Unbound Medicine ER -