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A novel dermal substitute based on biofunctionalized electrospun PCL nanofibrous matrix.
J Biomed Mater Res A. 2011 Sep 01; 98(3):461-72.JB

Abstract

In this study, nanofibrous matrices of polycaprolactone (PCL) and PCL/collagen with immobilized epidermal growth factor (EGF) were successfully fabricated by electrospinning for the purpose of damaged skin regeneration. Nanofiber diameters were found to be 284 ± 48 nm for PCL and 330 ± 104 nm for PCL/collagen matrices. The porosities were calculated as 85% for PCL and 90% for PCL/collagen matrices. The covalent immobilization of EGF onto the nanofibrous matrices was verified by the increase of surface atomic nitrogen ratio from 1.0 to 2.4% for PCL and from 3.7 to 4.7% for PCL/collagen. Moreover, EGF immobilization efficiencies of PCL and PCL/collagen matrices were determined as 98.5 and 99.2%, respectively. Human dermal keratinocytes (HS2) were cultivated on both neat and EGF immobilized PCL and PCL/collagen matrices to investigate the effects of matrix chemical composition and presence of EGF on cell proliferation and differentiation. EGF immobilized PCL/collagen matrices exerted early cell spreading and rapid proliferation. Statistically high expression levels of loricrin in HS2 cells cultivated on EGF immobilized PCL/collagen matrices were (p < 0.001) regarding superior differentiation ability of these cells compared to HS2 cells cultured on neat PCL and PCL/collagen matrices. In conclusion, this novel EGF immobilized PCL/collagen nanofibrous matrix could potentially be considered as an alternative dermal substitutes and wound healing material for skin tissue engineering applications.

Authors+Show Affiliations

Department of Bioengineering, Hacettepe University, Ankara, Turkey. menemse@hacettepe.edu.trNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

21661095

Citation

Gümüşderelioğlu, Menemşe, et al. "A Novel Dermal Substitute Based On Biofunctionalized Electrospun PCL Nanofibrous Matrix." Journal of Biomedical Materials Research. Part A, vol. 98, no. 3, 2011, pp. 461-72.
Gümüşderelioğlu M, Dalkıranoğlu S, Aydın RS, et al. A novel dermal substitute based on biofunctionalized electrospun PCL nanofibrous matrix. J Biomed Mater Res A. 2011;98(3):461-72.
Gümüşderelioğlu, M., Dalkıranoğlu, S., Aydın, R. S., & Cakmak, S. (2011). A novel dermal substitute based on biofunctionalized electrospun PCL nanofibrous matrix. Journal of Biomedical Materials Research. Part A, 98(3), 461-72. https://doi.org/10.1002/jbm.a.33143
Gümüşderelioğlu M, et al. A Novel Dermal Substitute Based On Biofunctionalized Electrospun PCL Nanofibrous Matrix. J Biomed Mater Res A. 2011 Sep 1;98(3):461-72. PubMed PMID: 21661095.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A novel dermal substitute based on biofunctionalized electrospun PCL nanofibrous matrix. AU - Gümüşderelioğlu,Menemşe, AU - Dalkıranoğlu,Sevcan, AU - Aydın,R Seda Tığlı, AU - Cakmak,Soner, Y1 - 2011/06/09/ PY - 2011/01/16/received PY - 2011/04/22/revised PY - 2011/04/25/accepted PY - 2011/6/11/entrez PY - 2011/6/11/pubmed PY - 2011/11/11/medline SP - 461 EP - 72 JF - Journal of biomedical materials research. Part A JO - J Biomed Mater Res A VL - 98 IS - 3 N2 - In this study, nanofibrous matrices of polycaprolactone (PCL) and PCL/collagen with immobilized epidermal growth factor (EGF) were successfully fabricated by electrospinning for the purpose of damaged skin regeneration. Nanofiber diameters were found to be 284 ± 48 nm for PCL and 330 ± 104 nm for PCL/collagen matrices. The porosities were calculated as 85% for PCL and 90% for PCL/collagen matrices. The covalent immobilization of EGF onto the nanofibrous matrices was verified by the increase of surface atomic nitrogen ratio from 1.0 to 2.4% for PCL and from 3.7 to 4.7% for PCL/collagen. Moreover, EGF immobilization efficiencies of PCL and PCL/collagen matrices were determined as 98.5 and 99.2%, respectively. Human dermal keratinocytes (HS2) were cultivated on both neat and EGF immobilized PCL and PCL/collagen matrices to investigate the effects of matrix chemical composition and presence of EGF on cell proliferation and differentiation. EGF immobilized PCL/collagen matrices exerted early cell spreading and rapid proliferation. Statistically high expression levels of loricrin in HS2 cells cultivated on EGF immobilized PCL/collagen matrices were (p < 0.001) regarding superior differentiation ability of these cells compared to HS2 cells cultured on neat PCL and PCL/collagen matrices. In conclusion, this novel EGF immobilized PCL/collagen nanofibrous matrix could potentially be considered as an alternative dermal substitutes and wound healing material for skin tissue engineering applications. SN - 1552-4965 UR - https://www.unboundmedicine.com/medline/citation/21661095/A_novel_dermal_substitute_based_on_biofunctionalized_electrospun_PCL_nanofibrous_matrix_ L2 - https://doi.org/10.1002/jbm.a.33143 DB - PRIME DP - Unbound Medicine ER -