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Bone turnover in passive smoking female rat: relationships to change in bone mineral density.
BMC Musculoskelet Disord. 2011 Jun 11; 12:131.BM

Abstract

BACKGROUND

Many studies have identified smoking as a risk factor for osteoporosis, but it is unclear whether passive smoking has an effect on bone mineral density and bone turnover and if such an effect could cause osteoporosis.The purpose of the study was to investigate the effect of passive smoking on bone mineral density (BMD) and bone turnover and the relationship between BMD and bone turnover in female rat.

METHODS

Forty-eight female Wistar rats were randomized into six groups: 2-month, 3-month,4-month smoke-exposed rats and their controls. A rat model of passive cigarette smoking was prepared by breeding female rats in a cigarette-smoking box for 2, 3 or 4 months. Serums were analyzed for levels of osteocalcin, bone-specific alkaline phosphatase (b-ALP) and Tartrate-resistant acid phosphatase 5b (TRACP 5b). BMD was assessed at lumbar vertebrae and femur by dual energy X-ray absorptiometry in passive smoking rats and in control rats.

RESULTS

BMD of lumbar spine and femur was lower in 4-month smoke-exposed female rats than that in controls. However, there was no significant difference in serum osteocalcin levels between smoke-exposed rats and controls. Significantly lower b-ALP and higher TRACP 5b were found in the 3-month or 4-month smoke-exposed rats compared to controls. Subsequent analysis showed that b-ALP positively correlated with BMD of the lumbar vertebrae(r = 0.764, P = 0.027) and femur(r = 0.899, P = 0.002) in 4-month smoke-exposed female rats. Furthermore, TRACP 5b levels negatively correlated with BMD of lumbar vertebrae (r = -0.871, P = 0.005) and femur (r = -0.715, P = 0.046) in 4-month smoke-exposed female rats.

CONCLUSION

Our data suggest that smoke exposure can inhibit bone formation and increase bone resorption. The hazardous effects of passive smoking on bone status are associated with increased bone turnover in female rat.

Authors+Show Affiliations

Department of Orthopaedics, Xiangya Hospital, Central South University, Changsha, Hunan, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21663694

Citation

Gao, Shu-guang, et al. "Bone Turnover in Passive Smoking Female Rat: Relationships to Change in Bone Mineral Density." BMC Musculoskeletal Disorders, vol. 12, 2011, p. 131.
Gao SG, Li KH, Xu M, et al. Bone turnover in passive smoking female rat: relationships to change in bone mineral density. BMC Musculoskelet Disord. 2011;12:131.
Gao, S. G., Li, K. H., Xu, M., Jiang, W., Shen, H., Luo, W., Xu, W. S., Tian, J., & Lei, G. H. (2011). Bone turnover in passive smoking female rat: relationships to change in bone mineral density. BMC Musculoskeletal Disorders, 12, 131. https://doi.org/10.1186/1471-2474-12-131
Gao SG, et al. Bone Turnover in Passive Smoking Female Rat: Relationships to Change in Bone Mineral Density. BMC Musculoskelet Disord. 2011 Jun 11;12:131. PubMed PMID: 21663694.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bone turnover in passive smoking female rat: relationships to change in bone mineral density. AU - Gao,Shu-guang, AU - Li,Kang-hua, AU - Xu,Mai, AU - Jiang,Wei, AU - Shen,Hong, AU - Luo,Wei, AU - Xu,Wen-shuo, AU - Tian,Jian, AU - Lei,Guang-hua, Y1 - 2011/06/11/ PY - 2010/12/26/received PY - 2011/06/11/accepted PY - 2011/6/14/entrez PY - 2011/6/15/pubmed PY - 2011/9/15/medline SP - 131 EP - 131 JF - BMC musculoskeletal disorders JO - BMC Musculoskelet Disord VL - 12 N2 - BACKGROUND: Many studies have identified smoking as a risk factor for osteoporosis, but it is unclear whether passive smoking has an effect on bone mineral density and bone turnover and if such an effect could cause osteoporosis.The purpose of the study was to investigate the effect of passive smoking on bone mineral density (BMD) and bone turnover and the relationship between BMD and bone turnover in female rat. METHODS: Forty-eight female Wistar rats were randomized into six groups: 2-month, 3-month,4-month smoke-exposed rats and their controls. A rat model of passive cigarette smoking was prepared by breeding female rats in a cigarette-smoking box for 2, 3 or 4 months. Serums were analyzed for levels of osteocalcin, bone-specific alkaline phosphatase (b-ALP) and Tartrate-resistant acid phosphatase 5b (TRACP 5b). BMD was assessed at lumbar vertebrae and femur by dual energy X-ray absorptiometry in passive smoking rats and in control rats. RESULTS: BMD of lumbar spine and femur was lower in 4-month smoke-exposed female rats than that in controls. However, there was no significant difference in serum osteocalcin levels between smoke-exposed rats and controls. Significantly lower b-ALP and higher TRACP 5b were found in the 3-month or 4-month smoke-exposed rats compared to controls. Subsequent analysis showed that b-ALP positively correlated with BMD of the lumbar vertebrae(r = 0.764, P = 0.027) and femur(r = 0.899, P = 0.002) in 4-month smoke-exposed female rats. Furthermore, TRACP 5b levels negatively correlated with BMD of lumbar vertebrae (r = -0.871, P = 0.005) and femur (r = -0.715, P = 0.046) in 4-month smoke-exposed female rats. CONCLUSION: Our data suggest that smoke exposure can inhibit bone formation and increase bone resorption. The hazardous effects of passive smoking on bone status are associated with increased bone turnover in female rat. SN - 1471-2474 UR - https://www.unboundmedicine.com/medline/citation/21663694/Bone_turnover_in_passive_smoking_female_rat:_relationships_to_change_in_bone_mineral_density_ L2 - https://bmcmusculoskeletdisord.biomedcentral.com/articles/10.1186/1471-2474-12-131 DB - PRIME DP - Unbound Medicine ER -