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Complete protection against a H5N2 avian influenza virus by a DNA vaccine expressing a fusion protein of H1N1 HA and M2e.
Vaccine. 2011 Jul 26; 29(33):5481-7.V

Abstract

Most influenza vaccines target hemagglutinin (HA) in order to protect the host against infection. However, theses vaccines are strain-specific due to major antigenic variations of HA. Since it is difficult to predict epidemic and pandemic strains of influenza virus, the development of effective vaccines against divergent influenza viruses is urgently needed. Although M2e-based vaccines are associated with weaker protection than HA-based vaccines that induce neutralizing antibodies against challenge virus matched-strain, the extracellular domain of Matrix 2 protein (M2e) is one of a potential broad-spectrum immunogen because it contains highly conserved sequences among influenza A viruses. In this study, M2e sequence was fused to H1N1 HA DNA (M2e-HA) and the immunogenicity and antiviral efficacy of this DNA vaccine was evaluated in response to challenge with a heterosubtypic H5N2 avian influenza virus. Compared to vaccination with HA or M2e DNA alone, vaccination with M2e-HA DNA or combination of M2e DNA and HA DNA (M2e DNA+HA DNA) induced a broad immunity without evidence of immune interference. In addition, HA-specific CD8(+) and M2e-specific T cell responses elicited by M2e-HA DNA vaccination were significantly higher than those of HA or M2e DNA vaccine alone, respectively. Following challenge with a heterosubtypic influenza virus infection, vaccination with M2e-HA DNA conferred complete protection against mortality. In combination, these results suggest that DNA vaccines expressing a fusion protein, M2e-HA, may provide an attractive approach for the development of broad-spectrum influenza vaccines.

Authors+Show Affiliations

Laboratory of Cellular Immunology, Division of Molecular and Life Sciences, POSTECH, Pohang 790-784, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21664216

Citation

Park, Ki Seok, et al. "Complete Protection Against a H5N2 Avian Influenza Virus By a DNA Vaccine Expressing a Fusion Protein of H1N1 HA and M2e." Vaccine, vol. 29, no. 33, 2011, pp. 5481-7.
Park KS, Seo YB, Lee JY, et al. Complete protection against a H5N2 avian influenza virus by a DNA vaccine expressing a fusion protein of H1N1 HA and M2e. Vaccine. 2011;29(33):5481-7.
Park, K. S., Seo, Y. B., Lee, J. Y., Im, S. J., Seo, S. H., Song, M. S., Choi, Y. K., & Sung, Y. C. (2011). Complete protection against a H5N2 avian influenza virus by a DNA vaccine expressing a fusion protein of H1N1 HA and M2e. Vaccine, 29(33), 5481-7. https://doi.org/10.1016/j.vaccine.2011.05.062
Park KS, et al. Complete Protection Against a H5N2 Avian Influenza Virus By a DNA Vaccine Expressing a Fusion Protein of H1N1 HA and M2e. Vaccine. 2011 Jul 26;29(33):5481-7. PubMed PMID: 21664216.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Complete protection against a H5N2 avian influenza virus by a DNA vaccine expressing a fusion protein of H1N1 HA and M2e. AU - Park,Ki Seok, AU - Seo,Yong Bok, AU - Lee,Ji Yeung, AU - Im,Se Jin, AU - Seo,Sang Hwan, AU - Song,Min Suk, AU - Choi,Young Ki, AU - Sung,Young Chul, Y1 - 2011/06/12/ PY - 2010/12/14/received PY - 2011/04/30/revised PY - 2011/05/18/accepted PY - 2011/6/14/entrez PY - 2011/6/15/pubmed PY - 2011/11/9/medline SP - 5481 EP - 7 JF - Vaccine JO - Vaccine VL - 29 IS - 33 N2 - Most influenza vaccines target hemagglutinin (HA) in order to protect the host against infection. However, theses vaccines are strain-specific due to major antigenic variations of HA. Since it is difficult to predict epidemic and pandemic strains of influenza virus, the development of effective vaccines against divergent influenza viruses is urgently needed. Although M2e-based vaccines are associated with weaker protection than HA-based vaccines that induce neutralizing antibodies against challenge virus matched-strain, the extracellular domain of Matrix 2 protein (M2e) is one of a potential broad-spectrum immunogen because it contains highly conserved sequences among influenza A viruses. In this study, M2e sequence was fused to H1N1 HA DNA (M2e-HA) and the immunogenicity and antiviral efficacy of this DNA vaccine was evaluated in response to challenge with a heterosubtypic H5N2 avian influenza virus. Compared to vaccination with HA or M2e DNA alone, vaccination with M2e-HA DNA or combination of M2e DNA and HA DNA (M2e DNA+HA DNA) induced a broad immunity without evidence of immune interference. In addition, HA-specific CD8(+) and M2e-specific T cell responses elicited by M2e-HA DNA vaccination were significantly higher than those of HA or M2e DNA vaccine alone, respectively. Following challenge with a heterosubtypic influenza virus infection, vaccination with M2e-HA DNA conferred complete protection against mortality. In combination, these results suggest that DNA vaccines expressing a fusion protein, M2e-HA, may provide an attractive approach for the development of broad-spectrum influenza vaccines. SN - 1873-2518 UR - https://www.unboundmedicine.com/medline/citation/21664216/Complete_protection_against_a_H5N2_avian_influenza_virus_by_a_DNA_vaccine_expressing_a_fusion_protein_of_H1N1_HA_and_M2e_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0264-410X(11)00782-1 DB - PRIME DP - Unbound Medicine ER -