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The origin of tobacco's T genome is traced to a particular lineage within Nicotiana tomentosiformis (Solanaceae).
Am J Bot 2002; 89(6):921-8AJ

Abstract

Nicotiana tabacum (tobacco) is a natural allotetraploid. The maternal genome donor is not controversial and is probably derived from an ancestor of N. sylvestris. The paternal, T-genome donor has been less clear, with N. tomentosiformis, N. otophora, or an introgression hybrid proposed. Here we provide evidence that the T genome of N. tabacum is derived from a particular lineage of N. tomentosiformis. We show that the repetitive sequences of geminiviral origin, GRD53 and GRD3, are present in the genomes of N. tabacum cultivars, a tobacco cell suspension culture TBY-2, and N. tomentosiformis ac. NIC 479/84. Surprisingly, they are not present in another three varieties of N. tomentosiformis. A detailed cytogenetic analysis also revealed that N. tomentosiformis ac. NIC 479/84 most closely resembles the N. tabacum T genome in the location of other tandem repetitive sequences. Thus, tobacco formed after divergence within N. tomentosiformis, and the spectrum of potential donors of the paternal genome can be narrowed to a genotype of N. tomentosiformis characterized by the presence of GRD53 and GRD3 repeats. It is clear that future paternity studies in tobacco should use N. tomentosiformis ac. NIC 479/84 rather than any other accession.

Authors+Show Affiliations

School of Biological Sciences, Queen Mary, University of London, London, E1 4NS, UK;No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

21665691

Citation

Murad, Lena, et al. "The Origin of Tobacco's T Genome Is Traced to a Particular Lineage Within Nicotiana Tomentosiformis (Solanaceae)." American Journal of Botany, vol. 89, no. 6, 2002, pp. 921-8.
Murad L, Lim KY, Christopodulou V, et al. The origin of tobacco's T genome is traced to a particular lineage within Nicotiana tomentosiformis (Solanaceae). Am J Bot. 2002;89(6):921-8.
Murad, L., Lim, K. Y., Christopodulou, V., Matyasek, R., Lichtenstein, C. P., Kovarik, A., & Leitch, A. R. (2002). The origin of tobacco's T genome is traced to a particular lineage within Nicotiana tomentosiformis (Solanaceae). American Journal of Botany, 89(6), pp. 921-8. doi:10.3732/ajb.89.6.921.
Murad L, et al. The Origin of Tobacco's T Genome Is Traced to a Particular Lineage Within Nicotiana Tomentosiformis (Solanaceae). Am J Bot. 2002;89(6):921-8. PubMed PMID: 21665691.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The origin of tobacco's T genome is traced to a particular lineage within Nicotiana tomentosiformis (Solanaceae). AU - Murad,Lena, AU - Lim,K Yoong, AU - Christopodulou,Vangellis, AU - Matyasek,Roman, AU - Lichtenstein,Conrad P, AU - Kovarik,Ales, AU - Leitch,Andrew R, PY - 2011/6/14/entrez PY - 2002/6/1/pubmed PY - 2002/6/1/medline SP - 921 EP - 8 JF - American journal of botany JO - Am. J. Bot. VL - 89 IS - 6 N2 - Nicotiana tabacum (tobacco) is a natural allotetraploid. The maternal genome donor is not controversial and is probably derived from an ancestor of N. sylvestris. The paternal, T-genome donor has been less clear, with N. tomentosiformis, N. otophora, or an introgression hybrid proposed. Here we provide evidence that the T genome of N. tabacum is derived from a particular lineage of N. tomentosiformis. We show that the repetitive sequences of geminiviral origin, GRD53 and GRD3, are present in the genomes of N. tabacum cultivars, a tobacco cell suspension culture TBY-2, and N. tomentosiformis ac. NIC 479/84. Surprisingly, they are not present in another three varieties of N. tomentosiformis. A detailed cytogenetic analysis also revealed that N. tomentosiformis ac. NIC 479/84 most closely resembles the N. tabacum T genome in the location of other tandem repetitive sequences. Thus, tobacco formed after divergence within N. tomentosiformis, and the spectrum of potential donors of the paternal genome can be narrowed to a genotype of N. tomentosiformis characterized by the presence of GRD53 and GRD3 repeats. It is clear that future paternity studies in tobacco should use N. tomentosiformis ac. NIC 479/84 rather than any other accession. SN - 0002-9122 UR - https://www.unboundmedicine.com/medline/citation/21665691/The_origin_of_tobacco's_T_genome_is_traced_to_a_particular_lineage_within_Nicotiana_tomentosiformis__Solanaceae__ L2 - https://doi.org/10.3732/ajb.89.6.921 DB - PRIME DP - Unbound Medicine ER -