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Dried blood spots on filter paper as an alternative specimen for measles diagnostics: detection of measles immunoglobulin M antibody by a commercial enzyme immunoassay.
J Infect Dis. 2011 Jul; 204 Suppl 1:S564-9.JI

Abstract

BACKGROUND

We compared the results of a serum-based measles immunoglobulin M (IgM) test with results of tests using paired reconstituted dried filter paper blood spot (DBS) samples to assess the feasibility of using DBS samples for measles diagnostic procedures.

METHODS

We collected 588 paired serum and DBS samples from 349 children aged 8 months through 12 years at Mulago Hospital in Kampala, Uganda; of these samples, 513 (87%) were collected from children with a clinical diagnosis of measles 0-33 days after rash, and 75(13%) were collected from children hospitalized for other reasons. Eluted DBS and serum samples were tested using a commercial measles IgM enzyme immunoassay. Detection of viral RNA was attempted on a subset of 20 DBS by reverse-transcriptase polymerase chain reaction.

RESULTS

Among the 513 sample pairs collected from children with measles, the concordances for samples collected during days 0-6 and >1 week after rash were 95.7% and 100%, respectively (P<.01). The relative sensitivity and specificity of the DBS-based assay during the first week were 98.7% and 88.9%, respectively, and the sensitivity and specificity >1 week after rash were 100% and 100%, respectively. Viral RNA was detected in 5 (26%) of 19 DBS samples tested. Among 75 sample pairs collected from children hospitalized for other reasons, concordance was 94.7%.

CONCLUSIONS

DBS samples are a feasible alternative sample for measles diagnostic procedures in high-incidence settings.

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Authors+Show Affiliations

Uzicanin A
Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA. aau5@cdc.gov
Lubega I
No affiliation info available
Nanuynja M
No affiliation info available
Mercader S
No affiliation info available
Rota P
No affiliation info available
Bellini W
No affiliation info available
Helfand R
No affiliation info available

MeSH

Antibodies, ViralCase-Control StudiesChildChild, PreschoolFemaleHumansImmunoenzyme TechniquesImmunoglobulin MInfantMaleMeaslesPaperSpecimen HandlingUganda

Pub Type(s)

Comparative Study
Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

21666214

Citation

Uzicanin, Amra, et al. "Dried Blood Spots On Filter Paper as an Alternative Specimen for Measles Diagnostics: Detection of Measles Immunoglobulin M Antibody By a Commercial Enzyme Immunoassay." The Journal of Infectious Diseases, vol. 204 Suppl 1, 2011, pp. S564-9.
Uzicanin A, Lubega I, Nanuynja M, et al. Dried blood spots on filter paper as an alternative specimen for measles diagnostics: detection of measles immunoglobulin M antibody by a commercial enzyme immunoassay. J Infect Dis. 2011;204 Suppl 1:S564-9.
Uzicanin, A., Lubega, I., Nanuynja, M., Mercader, S., Rota, P., Bellini, W., & Helfand, R. (2011). Dried blood spots on filter paper as an alternative specimen for measles diagnostics: detection of measles immunoglobulin M antibody by a commercial enzyme immunoassay. The Journal of Infectious Diseases, 204 Suppl 1, S564-9. https://doi.org/10.1093/infdis/jir088
Uzicanin A, et al. Dried Blood Spots On Filter Paper as an Alternative Specimen for Measles Diagnostics: Detection of Measles Immunoglobulin M Antibody By a Commercial Enzyme Immunoassay. J Infect Dis. 2011;204 Suppl 1:S564-9. PubMed PMID: 21666214.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dried blood spots on filter paper as an alternative specimen for measles diagnostics: detection of measles immunoglobulin M antibody by a commercial enzyme immunoassay. AU - Uzicanin,Amra, AU - Lubega,Irene, AU - Nanuynja,Miriam, AU - Mercader,Sara, AU - Rota,Paul, AU - Bellini,William, AU - Helfand,Rita, PY - 2011/6/14/entrez PY - 2011/6/17/pubmed PY - 2011/7/21/medline SP - S564 EP - 9 JF - The Journal of infectious diseases JO - J Infect Dis VL - 204 Suppl 1 N2 - BACKGROUND: We compared the results of a serum-based measles immunoglobulin M (IgM) test with results of tests using paired reconstituted dried filter paper blood spot (DBS) samples to assess the feasibility of using DBS samples for measles diagnostic procedures. METHODS: We collected 588 paired serum and DBS samples from 349 children aged 8 months through 12 years at Mulago Hospital in Kampala, Uganda; of these samples, 513 (87%) were collected from children with a clinical diagnosis of measles 0-33 days after rash, and 75(13%) were collected from children hospitalized for other reasons. Eluted DBS and serum samples were tested using a commercial measles IgM enzyme immunoassay. Detection of viral RNA was attempted on a subset of 20 DBS by reverse-transcriptase polymerase chain reaction. RESULTS: Among the 513 sample pairs collected from children with measles, the concordances for samples collected during days 0-6 and >1 week after rash were 95.7% and 100%, respectively (P<.01). The relative sensitivity and specificity of the DBS-based assay during the first week were 98.7% and 88.9%, respectively, and the sensitivity and specificity >1 week after rash were 100% and 100%, respectively. Viral RNA was detected in 5 (26%) of 19 DBS samples tested. Among 75 sample pairs collected from children hospitalized for other reasons, concordance was 94.7%. CONCLUSIONS: DBS samples are a feasible alternative sample for measles diagnostic procedures in high-incidence settings. SN - 1537-6613 UR - https://www.unboundmedicine.com/medline/citation/21666214/Dried_blood_spots_on_filter_paper_as_an_alternative_specimen_for_measles_diagnostics:_detection_of_measles_immunoglobulin_M_antibody_by_a_commercial_enzyme_immunoassay_ L2 - https://academic.oup.com/jid/article-lookup/doi/10.1093/infdis/jir088 DB - PRIME DP - Unbound Medicine ER -