Tags

Type your tag names separated by a space and hit enter

Methyl-1-hydroxy-2-naphthoate, a novel naphthol derivative, inhibits lipopolysaccharide-induced inflammatory response in macrophages via suppression of NF-κB, JNK and p38 MAPK pathways.
Inflamm Res 2011; 60(9):851-9IR

Abstract

OBJECTIVE AND DESIGN

The anti-inflammatory effect of methyl-1-hydroxy-2-naphthoate (MHNA), a novel naphthol derivative, was evaluated in the lipopolysaccharide (LPS)-induced inflammatory response in murine macrophages.

MATERIALS AND METHODS

The release of nitric oxide (NO), interleukin-1beta (IL-1β) and interleukin-6 (IL-6) were detected by the Griess reagent and ELISA methods. The protein expressions of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) were examined by Western blotting. The mRNA expressions of IL-1β, IL-6, iNOS and COX-2 were determined by real-time PCR. Activation of mitogen-activated protein kinases (MAPKs) and nuclear factor kappa B (NF-κB) pathways were detected by Western blotting, reporter gene assay and electrophoretic mobility shift assay.

RESULTS

MHNA significantly inhibited the release of NO, IL-1β and IL-6 as well as the protein expression of iNOS and COX-2 in LPS-stimulated macrophages. It also inhibited the mRNA expression of iNOS, COX-2, IL-1β and IL-6. Further studies indicated that MHNA inhibited LPS-induced increases in NF-κB DNA-binding activity and NF-κB transcriptional activity as well as IκB-α degradation and NF-κB translocation in a dose-dependent manner. Meanwhile, the activation of p38 MAPK and c-Jun N-terminal kinases (JNK) induced by LPS were decreased by MHNA.

CONCLUSIONS

MHNA inhibits the LPS-induced inflammatory response in murine macrophages via suppression of NF-κB and MAPKs signaling pathways activation.

Authors+Show Affiliations

School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, 510515, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21667204

Citation

Zhang, Jun-Yan, et al. "Methyl-1-hydroxy-2-naphthoate, a Novel Naphthol Derivative, Inhibits Lipopolysaccharide-induced Inflammatory Response in Macrophages Via Suppression of NF-κB, JNK and P38 MAPK Pathways." Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.], vol. 60, no. 9, 2011, pp. 851-9.
Zhang JY, Jin H, Wang GF, et al. Methyl-1-hydroxy-2-naphthoate, a novel naphthol derivative, inhibits lipopolysaccharide-induced inflammatory response in macrophages via suppression of NF-κB, JNK and p38 MAPK pathways. Inflamm Res. 2011;60(9):851-9.
Zhang, J. Y., Jin, H., Wang, G. F., Yu, P. J., Wu, S. Y., Zhu, Z. G., ... Wu, S. G. (2011). Methyl-1-hydroxy-2-naphthoate, a novel naphthol derivative, inhibits lipopolysaccharide-induced inflammatory response in macrophages via suppression of NF-κB, JNK and p38 MAPK pathways. Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.], 60(9), pp. 851-9. doi:10.1007/s00011-011-0345-2.
Zhang JY, et al. Methyl-1-hydroxy-2-naphthoate, a Novel Naphthol Derivative, Inhibits Lipopolysaccharide-induced Inflammatory Response in Macrophages Via Suppression of NF-κB, JNK and P38 MAPK Pathways. Inflamm Res. 2011;60(9):851-9. PubMed PMID: 21667204.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Methyl-1-hydroxy-2-naphthoate, a novel naphthol derivative, inhibits lipopolysaccharide-induced inflammatory response in macrophages via suppression of NF-κB, JNK and p38 MAPK pathways. AU - Zhang,Jun-Yan, AU - Jin,Hong, AU - Wang,Guang-Fa, AU - Yu,Peng-Jiu, AU - Wu,Shao-Yu, AU - Zhu,Zheng-Guang, AU - Li,Zhong-Huang, AU - Tian,Yuan-Xin, AU - Xu,Wei, AU - Zhang,Jia-Jie, AU - Wu,Shu-Guang, Y1 - 2011/06/11/ PY - 2010/10/19/received PY - 2011/05/05/accepted PY - 2011/04/01/revised PY - 2011/6/14/entrez PY - 2011/6/15/pubmed PY - 2012/2/14/medline SP - 851 EP - 9 JF - Inflammation research : official journal of the European Histamine Research Society ... [et al.] JO - Inflamm. Res. VL - 60 IS - 9 N2 - OBJECTIVE AND DESIGN: The anti-inflammatory effect of methyl-1-hydroxy-2-naphthoate (MHNA), a novel naphthol derivative, was evaluated in the lipopolysaccharide (LPS)-induced inflammatory response in murine macrophages. MATERIALS AND METHODS: The release of nitric oxide (NO), interleukin-1beta (IL-1β) and interleukin-6 (IL-6) were detected by the Griess reagent and ELISA methods. The protein expressions of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) were examined by Western blotting. The mRNA expressions of IL-1β, IL-6, iNOS and COX-2 were determined by real-time PCR. Activation of mitogen-activated protein kinases (MAPKs) and nuclear factor kappa B (NF-κB) pathways were detected by Western blotting, reporter gene assay and electrophoretic mobility shift assay. RESULTS: MHNA significantly inhibited the release of NO, IL-1β and IL-6 as well as the protein expression of iNOS and COX-2 in LPS-stimulated macrophages. It also inhibited the mRNA expression of iNOS, COX-2, IL-1β and IL-6. Further studies indicated that MHNA inhibited LPS-induced increases in NF-κB DNA-binding activity and NF-κB transcriptional activity as well as IκB-α degradation and NF-κB translocation in a dose-dependent manner. Meanwhile, the activation of p38 MAPK and c-Jun N-terminal kinases (JNK) induced by LPS were decreased by MHNA. CONCLUSIONS: MHNA inhibits the LPS-induced inflammatory response in murine macrophages via suppression of NF-κB and MAPKs signaling pathways activation. SN - 1420-908X UR - https://www.unboundmedicine.com/medline/citation/21667204/Methyl_1_hydroxy_2_naphthoate_a_novel_naphthol_derivative_inhibits_lipopolysaccharide_induced_inflammatory_response_in_macrophages_via_suppression_of_NF_κB_JNK_and_p38_MAPK_pathways_ L2 - https://dx.doi.org/10.1007/s00011-011-0345-2 DB - PRIME DP - Unbound Medicine ER -