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Gain and loss of extracellular molecules in sporadic inclusion body myositis and polymyositis--a proteomics-based study.
Brain Pathol 2012; 22(1):32-40BP

Abstract

Sporadic inclusion body myositis (sIBM) contains non-necrotic myofibers that are surrounded and/or invaded by inflammatory cells. In this study we aimed to identify selective molecules that are present at this site. Myofibers of four biopsies of sIBM that were surrounded and/or invaded by inflammatory cells were microdissected, pooled and profiled by proteomic studies using mass spectrometry. Normal skeletal muscle tissue served as control. Based on the table of proteins that were detected in sIBM only, we selected nine extracellular matrix molecules and validated the results performing immunofluorescence. Seven out of nine proteins that were detected in sIBM by mass spectrometry showed different immunohistochemical results in myositis and normal controls. Of these, the small leucine-rich repeat proteins proline arginine-rich end leucine-rich repeat protein (PRELP) and biglycan were deposited precisely at myofibers surrounded and/or invaded by inflammatory cells both in sIBM and polymyositis. The basement membrane (BM) molecules merosin, perlecan, nidogen-2 and collagen IV were variably destroyed or increased at these sites. P component, which ensheathed all myofibers in normal controls, was absent from invaded myofibers. Similar to BM remodeling, the specific deposition of PRELP and biglycan may represent a mechanism to defend against immune attack. Loss of P component may affect the anchorage of the myofiber in the endomysium.

Authors+Show Affiliations

Department of Pathology and Neuropathology, Divison of Neuropathology, University Hospital of Tübingen, Tübingen, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

21672074

Citation

Doppler, Kathrin, et al. "Gain and Loss of Extracellular Molecules in Sporadic Inclusion Body Myositis and Polymyositis--a Proteomics-based Study." Brain Pathology (Zurich, Switzerland), vol. 22, no. 1, 2012, pp. 32-40.
Doppler K, Lindner A, Schütz W, et al. Gain and loss of extracellular molecules in sporadic inclusion body myositis and polymyositis--a proteomics-based study. Brain Pathol. 2012;22(1):32-40.
Doppler, K., Lindner, A., Schütz, W., Schütz, M., & Bornemann, A. (2012). Gain and loss of extracellular molecules in sporadic inclusion body myositis and polymyositis--a proteomics-based study. Brain Pathology (Zurich, Switzerland), 22(1), pp. 32-40. doi:10.1111/j.1750-3639.2011.00510.x.
Doppler K, et al. Gain and Loss of Extracellular Molecules in Sporadic Inclusion Body Myositis and Polymyositis--a Proteomics-based Study. Brain Pathol. 2012;22(1):32-40. PubMed PMID: 21672074.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Gain and loss of extracellular molecules in sporadic inclusion body myositis and polymyositis--a proteomics-based study. AU - Doppler,Kathrin, AU - Lindner,Alfred, AU - Schütz,Wolfgang, AU - Schütz,Monika, AU - Bornemann,Antje, Y1 - 2011/08/16/ PY - 2011/6/16/entrez PY - 2011/6/16/pubmed PY - 2012/8/21/medline SP - 32 EP - 40 JF - Brain pathology (Zurich, Switzerland) JO - Brain Pathol. VL - 22 IS - 1 N2 - Sporadic inclusion body myositis (sIBM) contains non-necrotic myofibers that are surrounded and/or invaded by inflammatory cells. In this study we aimed to identify selective molecules that are present at this site. Myofibers of four biopsies of sIBM that were surrounded and/or invaded by inflammatory cells were microdissected, pooled and profiled by proteomic studies using mass spectrometry. Normal skeletal muscle tissue served as control. Based on the table of proteins that were detected in sIBM only, we selected nine extracellular matrix molecules and validated the results performing immunofluorescence. Seven out of nine proteins that were detected in sIBM by mass spectrometry showed different immunohistochemical results in myositis and normal controls. Of these, the small leucine-rich repeat proteins proline arginine-rich end leucine-rich repeat protein (PRELP) and biglycan were deposited precisely at myofibers surrounded and/or invaded by inflammatory cells both in sIBM and polymyositis. The basement membrane (BM) molecules merosin, perlecan, nidogen-2 and collagen IV were variably destroyed or increased at these sites. P component, which ensheathed all myofibers in normal controls, was absent from invaded myofibers. Similar to BM remodeling, the specific deposition of PRELP and biglycan may represent a mechanism to defend against immune attack. Loss of P component may affect the anchorage of the myofiber in the endomysium. SN - 1750-3639 UR - https://www.unboundmedicine.com/medline/citation/21672074/full_citation/Gain_and_loss_of_extracellular_molecules_in_sporadic_inclusion_body_myositis_and_polymyositis___a_proteomics_based_study_ L2 - https://doi.org/10.1111/j.1750-3639.2011.00510.x DB - PRIME DP - Unbound Medicine ER -