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Inhibition effect of Gynura procumbens extract on UV-B-induced matrix-metalloproteinase expression in human dermal fibroblasts.
J Ethnopharmacol. 2011 Sep 01; 137(1):427-33.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Gynura procumbens Merr. (Asteraceae) has been used as a traditional remedy for various skin diseases in certain areas of Southeast Asia.

AIM OF THE STUDY

In order to evaluate the protective activity of Gynura procumbens extract on skin photoaging and elucidate its mode of action.

MATERIALS AND METHODS

Matrix-metalloproteinase (MMP)-1 and -9 expressions were induced by UV-B irradiation in human primary dermal fibroblasts. MMP-1 expression level was measured by enzyme-linked immunosorbent assay (ELISA) and Western blot analysis. Zymography was employed for evaluating the enzymatic activity of MMP-9. Anti-inflammatory activity and anti-oxidative capacity of the extract were evaluated by ELISA and dichlorodihydrofluorescein diacetate (DCF-DA) assay.

RESULTS

The ethanolic extract of Gynura procumbens inhibited MMP-1 expression up to 70% compare to negative control group. The enzymatic activity of MMP-9 was inhibited around 73% by the treatment of 20μg/mL of the extract. The extract markedly reduced the production of reactive oxygen species (ROS). Gynura procumbens extract showed an inhibitory effect on releasing pro-inflammatory cytokines (IL-6 and IL-8) in human HaCat keratinocyte.

CONCLUSION

The ethanolic extract of Gynura procumbens inhibited MMP-1 and MMP-9 expressions induced by UV-B irradiation via inhibition of pro-inflammatory cytokine mediator release and ROS production.

Authors+Show Affiliations

Amore-Pacific Co. R&D Center, 314-1, Bora-dong, Giheung-gu, Yongin-si, Gyeonggi-Do 446-729, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21672616

Citation

Kim, Junoh, et al. "Inhibition Effect of Gynura Procumbens Extract On UV-B-induced Matrix-metalloproteinase Expression in Human Dermal Fibroblasts." Journal of Ethnopharmacology, vol. 137, no. 1, 2011, pp. 427-33.
Kim J, Lee CW, Kim EK, et al. Inhibition effect of Gynura procumbens extract on UV-B-induced matrix-metalloproteinase expression in human dermal fibroblasts. J Ethnopharmacol. 2011;137(1):427-33.
Kim, J., Lee, C. W., Kim, E. K., Lee, S. J., Park, N. H., Kim, H. S., Kim, H. K., Char, K., Jang, Y. P., & Kim, J. W. (2011). Inhibition effect of Gynura procumbens extract on UV-B-induced matrix-metalloproteinase expression in human dermal fibroblasts. Journal of Ethnopharmacology, 137(1), 427-33. https://doi.org/10.1016/j.jep.2011.04.072
Kim J, et al. Inhibition Effect of Gynura Procumbens Extract On UV-B-induced Matrix-metalloproteinase Expression in Human Dermal Fibroblasts. J Ethnopharmacol. 2011 Sep 1;137(1):427-33. PubMed PMID: 21672616.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibition effect of Gynura procumbens extract on UV-B-induced matrix-metalloproteinase expression in human dermal fibroblasts. AU - Kim,Junoh, AU - Lee,Chan-Woo, AU - Kim,Eun Kyung, AU - Lee,Su-Jin, AU - Park,Nok-Hyun, AU - Kim,Han-Sung, AU - Kim,Han-Kon, AU - Char,Kookheon, AU - Jang,Young Pyo, AU - Kim,Jin-Woong, Y1 - 2011/06/06/ PY - 2010/12/15/received PY - 2011/04/20/accepted PY - 2011/6/16/entrez PY - 2011/6/16/pubmed PY - 2011/12/28/medline SP - 427 EP - 33 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 137 IS - 1 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Gynura procumbens Merr. (Asteraceae) has been used as a traditional remedy for various skin diseases in certain areas of Southeast Asia. AIM OF THE STUDY: In order to evaluate the protective activity of Gynura procumbens extract on skin photoaging and elucidate its mode of action. MATERIALS AND METHODS: Matrix-metalloproteinase (MMP)-1 and -9 expressions were induced by UV-B irradiation in human primary dermal fibroblasts. MMP-1 expression level was measured by enzyme-linked immunosorbent assay (ELISA) and Western blot analysis. Zymography was employed for evaluating the enzymatic activity of MMP-9. Anti-inflammatory activity and anti-oxidative capacity of the extract were evaluated by ELISA and dichlorodihydrofluorescein diacetate (DCF-DA) assay. RESULTS: The ethanolic extract of Gynura procumbens inhibited MMP-1 expression up to 70% compare to negative control group. The enzymatic activity of MMP-9 was inhibited around 73% by the treatment of 20μg/mL of the extract. The extract markedly reduced the production of reactive oxygen species (ROS). Gynura procumbens extract showed an inhibitory effect on releasing pro-inflammatory cytokines (IL-6 and IL-8) in human HaCat keratinocyte. CONCLUSION: The ethanolic extract of Gynura procumbens inhibited MMP-1 and MMP-9 expressions induced by UV-B irradiation via inhibition of pro-inflammatory cytokine mediator release and ROS production. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/21672616/Inhibition_effect_of_Gynura_procumbens_extract_on_UV_B_induced_matrix_metalloproteinase_expression_in_human_dermal_fibroblasts_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(11)00416-8 DB - PRIME DP - Unbound Medicine ER -