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Comparative analyses of chromatographic fingerprints of the roots of Polygonum multiflorum Thunb. and their processed products using RRLC/DAD/ESI-MS(n).
Planta Med. 2011 Nov; 77(16):1855-60.PM

Abstract

The dried roots of Polygonum multiflorum Thunb. (Heshouwu) and their processed products (Zhi-heshouwu) are widely used in traditional Chinese medicine, yet their therapeutic effects are different. Previous investigations focused mainly on the differences between Heshouwu and Zhi-heshouwu in the contents of several known compounds. In this study, a rapid resolution liquid chromatography-diode array detection/electrospray ionization tandem mass spectrometry (RRLC/DAD/ESI-MS(n)) method was developed for the comparative analysis of the components of Heshouwu and Zhi-heshouwu. A total of 23 compounds were identified or tentatively characterized. We found that 16 batches of Heshouwu and 15 batches of Zhi-heshouwu samples shared eight compounds, including gallic acid; 3,5,4'-tetrahydroxylstilbene-2,3-di-O-glucoside, CIS-2,3,5,4'-tetrahydroxylstilbene-2-O- β-D-glucoside, trans-2,3,5,4'-tetrahydroxylstilbene-2-O- β-D-glucoside, emodin-8-O- β-D-glucoside, physcion-8-O- β-D-glucoside, emodin, and physcion. Nevertheless, the relative amounts of gallic acid, emodin, and physcion were very high in Zhi-heshouwu samples compared to those in Heshouwu samples. Six compounds disappeared after processing and were unique for Heshouwu: catechin, flavanol gallate dimer, polygonimitin B, emodin-1-O-glucoside, emodin-8-O-(6'-O-malonyl)-glucoside, and physcion-8-O-(6'-O-malonyl)-glucoside. Three compounds were unique for Zhi-heshouwu: hydroxymaltol, 2,3-dihydro-3,5-dihydroxy-6-methyl-4(H)-pyran-4-one, and 5-hydroxymethyl furfural. These results suggest that the types and relative amounts of the chemical components of Heshouwu and Zhi-heshouwu are different.

Authors+Show Affiliations

Institution of Basic Theory, China Academy of Chinese Medical Sciences, Beijing, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21674436

Citation

Liu, Zhenli, et al. "Comparative Analyses of Chromatographic Fingerprints of the Roots of Polygonum Multiflorum Thunb. and Their Processed Products Using RRLC/DAD/ESI-MS(n)." Planta Medica, vol. 77, no. 16, 2011, pp. 1855-60.
Liu Z, Liu Y, Wang C, et al. Comparative analyses of chromatographic fingerprints of the roots of Polygonum multiflorum Thunb. and their processed products using RRLC/DAD/ESI-MS(n). Planta Med. 2011;77(16):1855-60.
Liu, Z., Liu, Y., Wang, C., Guo, N., Song, Z., Wang, C., Xia, L., & Lu, A. (2011). Comparative analyses of chromatographic fingerprints of the roots of Polygonum multiflorum Thunb. and their processed products using RRLC/DAD/ESI-MS(n). Planta Medica, 77(16), 1855-60. https://doi.org/10.1055/s-0030-1271200
Liu Z, et al. Comparative Analyses of Chromatographic Fingerprints of the Roots of Polygonum Multiflorum Thunb. and Their Processed Products Using RRLC/DAD/ESI-MS(n). Planta Med. 2011;77(16):1855-60. PubMed PMID: 21674436.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparative analyses of chromatographic fingerprints of the roots of Polygonum multiflorum Thunb. and their processed products using RRLC/DAD/ESI-MS(n). AU - Liu,Zhenli, AU - Liu,Yuanyan, AU - Wang,Chao, AU - Guo,Na, AU - Song,Zhiqian, AU - Wang,Chun, AU - Xia,Lei, AU - Lu,Aiping, Y1 - 2011/06/14/ PY - 2011/6/16/entrez PY - 2011/6/16/pubmed PY - 2013/4/11/medline SP - 1855 EP - 60 JF - Planta medica JO - Planta Med VL - 77 IS - 16 N2 - The dried roots of Polygonum multiflorum Thunb. (Heshouwu) and their processed products (Zhi-heshouwu) are widely used in traditional Chinese medicine, yet their therapeutic effects are different. Previous investigations focused mainly on the differences between Heshouwu and Zhi-heshouwu in the contents of several known compounds. In this study, a rapid resolution liquid chromatography-diode array detection/electrospray ionization tandem mass spectrometry (RRLC/DAD/ESI-MS(n)) method was developed for the comparative analysis of the components of Heshouwu and Zhi-heshouwu. A total of 23 compounds were identified or tentatively characterized. We found that 16 batches of Heshouwu and 15 batches of Zhi-heshouwu samples shared eight compounds, including gallic acid; 3,5,4'-tetrahydroxylstilbene-2,3-di-O-glucoside, CIS-2,3,5,4'-tetrahydroxylstilbene-2-O- β-D-glucoside, trans-2,3,5,4'-tetrahydroxylstilbene-2-O- β-D-glucoside, emodin-8-O- β-D-glucoside, physcion-8-O- β-D-glucoside, emodin, and physcion. Nevertheless, the relative amounts of gallic acid, emodin, and physcion were very high in Zhi-heshouwu samples compared to those in Heshouwu samples. Six compounds disappeared after processing and were unique for Heshouwu: catechin, flavanol gallate dimer, polygonimitin B, emodin-1-O-glucoside, emodin-8-O-(6'-O-malonyl)-glucoside, and physcion-8-O-(6'-O-malonyl)-glucoside. Three compounds were unique for Zhi-heshouwu: hydroxymaltol, 2,3-dihydro-3,5-dihydroxy-6-methyl-4(H)-pyran-4-one, and 5-hydroxymethyl furfural. These results suggest that the types and relative amounts of the chemical components of Heshouwu and Zhi-heshouwu are different. SN - 1439-0221 UR - https://www.unboundmedicine.com/medline/citation/21674436/Comparative_analyses_of_chromatographic_fingerprints_of_the_roots_of_Polygonum_multiflorum_Thunb__and_their_processed_products_using_RRLC/DAD/ESI_MS_n__ L2 - http://www.thieme-connect.com/DOI/DOI?10.1055/s-0030-1271200 DB - PRIME DP - Unbound Medicine ER -