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High temperature triggers the metabolism of S-nitrosothiols in sunflower mediating a process of nitrosative stress which provokes the inhibition of ferredoxin-NADP reductase by tyrosine nitration.
Plant Cell Environ. 2011 Nov; 34(11):1803-18.PC

Abstract

High temperature (HT) is considered a major abiotic stress that negatively affects both vegetative and reproductive growth. Whereas the metabolism of reactive oxygen species (ROS) is well established under HT, less is known about the metabolism of reactive nitrogen species (RNS). In sunflower (Helianthus annuus L.) seedlings exposed to HT, NO content as well as S-nitrosoglutathione reductase (GSNOR) activity and expression were down-regulated with the simultaneous accumulation of total S-nitrosothiols (SNOs) including S-nitrosoglutathione (GSNO). However, the content of tyrosine nitration (NO(2) -Tyr) studied by high-performance liquid chromatography with tandem mass spectrometry (LC-MS/MS) and by confocal laser scanning microscope was induced. Nitroproteome analysis under HT showed that this stress induced the protein expression of 13 tyrosine-nitrated proteins. Among the induced proteins, ferredoxin-NADP reductase (FNR) was selected to evaluate the effect of nitration on its activity after heat stress and in vitro conditions using 3-morpholinosydnonimine (SIN-1) (peroxynitrite donor) as the nitrating agent, the FNR activity being inhibited. Taken together, these results suggest that HT augments SNOs, which appear to mediate protein tyrosine nitration, inhibiting FNR, which is involved in the photosynthesis process.

Authors+Show Affiliations

Grupo de Señalización Molecular y Sistemas Antioxidantes en Plantas, Unidad Asociada al CSIC, Departamento de Bioquímica y Biología Molecular, Universidad de Jaén E-23071 Jaén, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21676000

Citation

Chaki, Mounira, et al. "High Temperature Triggers the Metabolism of S-nitrosothiols in Sunflower Mediating a Process of Nitrosative Stress Which Provokes the Inhibition of ferredoxin-NADP Reductase By Tyrosine Nitration." Plant, Cell & Environment, vol. 34, no. 11, 2011, pp. 1803-18.
Chaki M, Valderrama R, Fernández-Ocaña AM, et al. High temperature triggers the metabolism of S-nitrosothiols in sunflower mediating a process of nitrosative stress which provokes the inhibition of ferredoxin-NADP reductase by tyrosine nitration. Plant Cell Environ. 2011;34(11):1803-18.
Chaki, M., Valderrama, R., Fernández-Ocaña, A. M., Carreras, A., Gómez-Rodríguez, M. V., López-Jaramillo, J., Begara-Morales, J. C., Sánchez-Calvo, B., Luque, F., Leterrier, M., Corpas, F. J., & Barroso, J. B. (2011). High temperature triggers the metabolism of S-nitrosothiols in sunflower mediating a process of nitrosative stress which provokes the inhibition of ferredoxin-NADP reductase by tyrosine nitration. Plant, Cell & Environment, 34(11), 1803-18. https://doi.org/10.1111/j.1365-3040.2011.02376.x
Chaki M, et al. High Temperature Triggers the Metabolism of S-nitrosothiols in Sunflower Mediating a Process of Nitrosative Stress Which Provokes the Inhibition of ferredoxin-NADP Reductase By Tyrosine Nitration. Plant Cell Environ. 2011;34(11):1803-18. PubMed PMID: 21676000.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High temperature triggers the metabolism of S-nitrosothiols in sunflower mediating a process of nitrosative stress which provokes the inhibition of ferredoxin-NADP reductase by tyrosine nitration. AU - Chaki,Mounira, AU - Valderrama,Raquel, AU - Fernández-Ocaña,Ana M, AU - Carreras,Alfonso, AU - Gómez-Rodríguez,Maria V, AU - López-Jaramillo,Javier, AU - Begara-Morales,Juan C, AU - Sánchez-Calvo,Beatriz, AU - Luque,Francisco, AU - Leterrier,Marina, AU - Corpas,Francisco J, AU - Barroso,Juan B, Y1 - 2011/07/13/ PY - 2011/6/17/entrez PY - 2011/6/17/pubmed PY - 2012/2/4/medline SP - 1803 EP - 18 JF - Plant, cell & environment JO - Plant Cell Environ VL - 34 IS - 11 N2 - High temperature (HT) is considered a major abiotic stress that negatively affects both vegetative and reproductive growth. Whereas the metabolism of reactive oxygen species (ROS) is well established under HT, less is known about the metabolism of reactive nitrogen species (RNS). In sunflower (Helianthus annuus L.) seedlings exposed to HT, NO content as well as S-nitrosoglutathione reductase (GSNOR) activity and expression were down-regulated with the simultaneous accumulation of total S-nitrosothiols (SNOs) including S-nitrosoglutathione (GSNO). However, the content of tyrosine nitration (NO(2) -Tyr) studied by high-performance liquid chromatography with tandem mass spectrometry (LC-MS/MS) and by confocal laser scanning microscope was induced. Nitroproteome analysis under HT showed that this stress induced the protein expression of 13 tyrosine-nitrated proteins. Among the induced proteins, ferredoxin-NADP reductase (FNR) was selected to evaluate the effect of nitration on its activity after heat stress and in vitro conditions using 3-morpholinosydnonimine (SIN-1) (peroxynitrite donor) as the nitrating agent, the FNR activity being inhibited. Taken together, these results suggest that HT augments SNOs, which appear to mediate protein tyrosine nitration, inhibiting FNR, which is involved in the photosynthesis process. SN - 1365-3040 UR - https://www.unboundmedicine.com/medline/citation/21676000/High_temperature_triggers_the_metabolism_of_S_nitrosothiols_in_sunflower_mediating_a_process_of_nitrosative_stress_which_provokes_the_inhibition_of_ferredoxin_NADP_reductase_by_tyrosine_nitration_ L2 - https://doi.org/10.1111/j.1365-3040.2011.02376.x DB - PRIME DP - Unbound Medicine ER -