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Identification of novel circulating coffee metabolites in human plasma by liquid chromatography-mass spectrometry.
J Chromatogr A. 2011 Jul 22; 1218(29):4678-88.JC

Abstract

This study reports a liquid chromatography-mass spectrometry method for the detection of polyphenol-derived metabolites in human plasma without enzymatic treatment after coffee consumption. Separation of available standards was achieved by reversed-phase ultra performance liquid chromatography and detection was performed by high resolution mass spectrometry in negative electrospray ionization mode. This analytical method was then applied for the identification and relative quantification of circulating coffee metabolites. A total of 34 coffee metabolites (mainly reduced, sulfated and methylated forms of caffeic acid, coumaric acid, caffeoylquinic acid and caffeoylquinic acid lactone) were identified based on mass accuracy (<4 ppm for most metabolites), specific fragmentation pattern and co-chromatography (when standard available). Among them, 19 circulating coffee metabolites were identified for the first time in human plasma such as feruloylquinic acid lactone, sulfated and glucuronidated forms of feruloylquinic acid lactone and sulfated forms of coumaric acid. Phenolic acid derivatives such as dihydroferulic acid, dihydroferulic acid 4'-O-sulfate, caffeic acid 3'-O-sulfate, dimethoxycinnamic acid, dihydrocaffeic acid and coumaric acid O-sulfate appeared to be the main metabolites circulating in human plasma after coffee consumption. The described method is a sensitive and reliable approach for the identification of coffee metabolites in biological fluids. In future, this analytical method will give more confidence in compound identification to provide a more comprehensive assessment of coffee polyphenol bioavailability studies in humans.

Authors+Show Affiliations

Metabolomics & Biomarkers Group, Nestlé Research Center, Nestec Ltd., Vers-chez-les-Blanc, 1000 Lausanne, Switzerland. Karine.Redeuil@rdls.nestle.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

21676405

Citation

Redeuil, Karine, et al. "Identification of Novel Circulating Coffee Metabolites in Human Plasma By Liquid Chromatography-mass Spectrometry." Journal of Chromatography. A, vol. 1218, no. 29, 2011, pp. 4678-88.
Redeuil K, Smarrito-Menozzi C, Guy P, et al. Identification of novel circulating coffee metabolites in human plasma by liquid chromatography-mass spectrometry. J Chromatogr A. 2011;1218(29):4678-88.
Redeuil, K., Smarrito-Menozzi, C., Guy, P., Rezzi, S., Dionisi, F., Williamson, G., Nagy, K., & Renouf, M. (2011). Identification of novel circulating coffee metabolites in human plasma by liquid chromatography-mass spectrometry. Journal of Chromatography. A, 1218(29), 4678-88. https://doi.org/10.1016/j.chroma.2011.05.050
Redeuil K, et al. Identification of Novel Circulating Coffee Metabolites in Human Plasma By Liquid Chromatography-mass Spectrometry. J Chromatogr A. 2011 Jul 22;1218(29):4678-88. PubMed PMID: 21676405.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of novel circulating coffee metabolites in human plasma by liquid chromatography-mass spectrometry. AU - Redeuil,Karine, AU - Smarrito-Menozzi,Candice, AU - Guy,Philippe, AU - Rezzi,Serge, AU - Dionisi,Fabiola, AU - Williamson,Gary, AU - Nagy,Kornél, AU - Renouf,Mathieu, Y1 - 2011/05/26/ PY - 2011/02/15/received PY - 2011/05/11/revised PY - 2011/05/15/accepted PY - 2011/6/17/entrez PY - 2011/6/17/pubmed PY - 2011/9/21/medline SP - 4678 EP - 88 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1218 IS - 29 N2 - This study reports a liquid chromatography-mass spectrometry method for the detection of polyphenol-derived metabolites in human plasma without enzymatic treatment after coffee consumption. Separation of available standards was achieved by reversed-phase ultra performance liquid chromatography and detection was performed by high resolution mass spectrometry in negative electrospray ionization mode. This analytical method was then applied for the identification and relative quantification of circulating coffee metabolites. A total of 34 coffee metabolites (mainly reduced, sulfated and methylated forms of caffeic acid, coumaric acid, caffeoylquinic acid and caffeoylquinic acid lactone) were identified based on mass accuracy (<4 ppm for most metabolites), specific fragmentation pattern and co-chromatography (when standard available). Among them, 19 circulating coffee metabolites were identified for the first time in human plasma such as feruloylquinic acid lactone, sulfated and glucuronidated forms of feruloylquinic acid lactone and sulfated forms of coumaric acid. Phenolic acid derivatives such as dihydroferulic acid, dihydroferulic acid 4'-O-sulfate, caffeic acid 3'-O-sulfate, dimethoxycinnamic acid, dihydrocaffeic acid and coumaric acid O-sulfate appeared to be the main metabolites circulating in human plasma after coffee consumption. The described method is a sensitive and reliable approach for the identification of coffee metabolites in biological fluids. In future, this analytical method will give more confidence in compound identification to provide a more comprehensive assessment of coffee polyphenol bioavailability studies in humans. SN - 1873-3778 UR - https://www.unboundmedicine.com/medline/citation/21676405/Identification_of_novel_circulating_coffee_metabolites_in_human_plasma_by_liquid_chromatography_mass_spectrometry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(11)00692-3 DB - PRIME DP - Unbound Medicine ER -