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Increasing prevalence and dissemination of NDM-1 metallo-β-lactamase in India: data from the SMART study (2009).
J Antimicrob Chemother. 2011 Sep; 66(9):1992-7.JA

Abstract

OBJECTIVES

To investigate the β-lactamase background of ertapenem non-susceptible isolates for the presence of the most commonly detected carbapenemase genes, bla(KPC), bla(OXA-48) and bla(VIM), and the newly described bla(NDM-1).

METHODS

Two hundred and thirty-five ertapenem-non-susceptible (MIC ≥ 0.5 mg/L) isolates of Enterobacteriaceae from the worldwide Study for Monitoring Antimicrobial Resistance Trends (SMART) 2009 programme were screened using a multiplex PCR for the presence of bla(KPC), bla(OXA-48), bla(VIM) and bla(NDM-1) genes. Extended-spectrum β-lactamase (ESBL) and AmpC genes (bla(ESBL) and bla(AmpC)) were identified using the Check-MDR CT101 microarray. DNA sequencing was performed to identify the bla(ESBL), bla(KPC) and bla(NDM-1) genes. Molecular typing was also performed to genetically characterize these isolates.

RESULTS

Sixty-six isolates (28%) had a carbapenemase gene, with bla(NDM-1) identified in 33 isolates including 2 isolates carrying both bla(NDM-1) and bla(OXA-48); other carbapenemase genes found included bla(KPC) (n = 23), bla(VIM) (n = 7) and bla(OXA-48) (n = 3). All bla(NDM-1)-carrying isolates were from patients in India and comprised five different species. With the exception of one isolate carrying only bla(NDM-1), all bla(NDM-1) carbapenemase-possessing isolates carried additional β-lactamases in various combinations: bla(ESBL) and bla(AmpC) (n = 18); bla(ESBL) (n = 10); bla(ESBL), bla(AmpC) and bla(OXA-48) (n = 2); and bla(AmpC) (n = 2). Except for bla(OXA-48)-carrying isolates, novel multilocus sequence types or enterobacterial repetitive intergenic consensus PCR patterns were observed along with clonal dissemination within and among sites.

CONCLUSIONS

A range of carbapenemase genes, associated with diverse ESBLs and/or AmpC backgrounds, were found among Enterobacteriaceae isolated during the study. Many of these ertapenem non-susceptible strains were clonally related and carried various combinations of β-lactamases.

Authors+Show Affiliations

International Health Management Associates, Inc., Schaumburg, IL 60173, USA. clascols@ihmainc.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

21676902

Citation

Lascols, Christine, et al. "Increasing Prevalence and Dissemination of NDM-1 Metallo-β-lactamase in India: Data From the SMART Study (2009)." The Journal of Antimicrobial Chemotherapy, vol. 66, no. 9, 2011, pp. 1992-7.
Lascols C, Hackel M, Marshall SH, et al. Increasing prevalence and dissemination of NDM-1 metallo-β-lactamase in India: data from the SMART study (2009). J Antimicrob Chemother. 2011;66(9):1992-7.
Lascols, C., Hackel, M., Marshall, S. H., Hujer, A. M., Bouchillon, S., Badal, R., Hoban, D., & Bonomo, R. A. (2011). Increasing prevalence and dissemination of NDM-1 metallo-β-lactamase in India: data from the SMART study (2009). The Journal of Antimicrobial Chemotherapy, 66(9), 1992-7. https://doi.org/10.1093/jac/dkr240
Lascols C, et al. Increasing Prevalence and Dissemination of NDM-1 Metallo-β-lactamase in India: Data From the SMART Study (2009). J Antimicrob Chemother. 2011;66(9):1992-7. PubMed PMID: 21676902.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Increasing prevalence and dissemination of NDM-1 metallo-β-lactamase in India: data from the SMART study (2009). AU - Lascols,Christine, AU - Hackel,Meredith, AU - Marshall,Steven H, AU - Hujer,Andrea M, AU - Bouchillon,Sam, AU - Badal,Robert, AU - Hoban,Daryl, AU - Bonomo,Robert A, Y1 - 2011/06/14/ PY - 2011/6/17/entrez PY - 2011/6/17/pubmed PY - 2011/10/15/medline SP - 1992 EP - 7 JF - The Journal of antimicrobial chemotherapy JO - J. Antimicrob. Chemother. VL - 66 IS - 9 N2 - OBJECTIVES: To investigate the β-lactamase background of ertapenem non-susceptible isolates for the presence of the most commonly detected carbapenemase genes, bla(KPC), bla(OXA-48) and bla(VIM), and the newly described bla(NDM-1). METHODS: Two hundred and thirty-five ertapenem-non-susceptible (MIC ≥ 0.5 mg/L) isolates of Enterobacteriaceae from the worldwide Study for Monitoring Antimicrobial Resistance Trends (SMART) 2009 programme were screened using a multiplex PCR for the presence of bla(KPC), bla(OXA-48), bla(VIM) and bla(NDM-1) genes. Extended-spectrum β-lactamase (ESBL) and AmpC genes (bla(ESBL) and bla(AmpC)) were identified using the Check-MDR CT101 microarray. DNA sequencing was performed to identify the bla(ESBL), bla(KPC) and bla(NDM-1) genes. Molecular typing was also performed to genetically characterize these isolates. RESULTS: Sixty-six isolates (28%) had a carbapenemase gene, with bla(NDM-1) identified in 33 isolates including 2 isolates carrying both bla(NDM-1) and bla(OXA-48); other carbapenemase genes found included bla(KPC) (n = 23), bla(VIM) (n = 7) and bla(OXA-48) (n = 3). All bla(NDM-1)-carrying isolates were from patients in India and comprised five different species. With the exception of one isolate carrying only bla(NDM-1), all bla(NDM-1) carbapenemase-possessing isolates carried additional β-lactamases in various combinations: bla(ESBL) and bla(AmpC) (n = 18); bla(ESBL) (n = 10); bla(ESBL), bla(AmpC) and bla(OXA-48) (n = 2); and bla(AmpC) (n = 2). Except for bla(OXA-48)-carrying isolates, novel multilocus sequence types or enterobacterial repetitive intergenic consensus PCR patterns were observed along with clonal dissemination within and among sites. CONCLUSIONS: A range of carbapenemase genes, associated with diverse ESBLs and/or AmpC backgrounds, were found among Enterobacteriaceae isolated during the study. Many of these ertapenem non-susceptible strains were clonally related and carried various combinations of β-lactamases. SN - 1460-2091 UR - https://www.unboundmedicine.com/medline/citation/21676902/Increasing_prevalence_and_dissemination_of_NDM_1_metallo_β_lactamase_in_India:_data_from_the_SMART_study__2009__ L2 - https://academic.oup.com/jac/article-lookup/doi/10.1093/jac/dkr240 DB - PRIME DP - Unbound Medicine ER -