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Role of yeast JmjC-domain containing histone demethylases in actively transcribed regions.
Biochem Biophys Res Commun. 2011 Jul 08; 410(3):614-9.BB

Abstract

In budding yeast, there are five JmjC domain-containing proteins, Jhd1, Jhd2, Rph1, Ecm5, and Gis1, which have been suggested to directly remove histone lysine methylation via a hydroxylation reaction. Of these demethylases, the ability of Jhd1 or Rph1 to demethylate histone H3 as a substrate has been identified in vivo. However, the overall roles of endogenous JmjC demethylases in the demethylation of histones encompassed by genes that are constitutively transcribed or their specificities towards histone H3 lysine modification at mono-, di-, or trimethylation states are still unclear. Using chromatin immunoprecipitation with nine specific antibodies directed against mono-, di-, or trimethylated histone H3 at lysines 4, 36, or 79, we show the whole patterns of histone H3 lysine methylation and the net changes in methylations that are caused by the deletion of each of the five JmjC demethylases in actively transcribed regions. Our results show that of the JmjC-containing proteins, Rph1 is the demethylase that is specific for histone H3K36 trimethylation during transcription elongation in vivo, and the abilities of other endogenous JmjC demethylasesto demethylate histone H3 are weak toward histone H3in actively transcribed regions.

Authors+Show Affiliations

Division of Molecular and Life Science, College of Science and Technology, Hanyang University, Ansan, Republic of Korea.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21684259

Citation

Kwon, Dae-Whan, and Seong Hoon Ahn. "Role of Yeast JmjC-domain Containing Histone Demethylases in Actively Transcribed Regions." Biochemical and Biophysical Research Communications, vol. 410, no. 3, 2011, pp. 614-9.
Kwon DW, Ahn SH. Role of yeast JmjC-domain containing histone demethylases in actively transcribed regions. Biochem Biophys Res Commun. 2011;410(3):614-9.
Kwon, D. W., & Ahn, S. H. (2011). Role of yeast JmjC-domain containing histone demethylases in actively transcribed regions. Biochemical and Biophysical Research Communications, 410(3), 614-9. https://doi.org/10.1016/j.bbrc.2011.06.039
Kwon DW, Ahn SH. Role of Yeast JmjC-domain Containing Histone Demethylases in Actively Transcribed Regions. Biochem Biophys Res Commun. 2011 Jul 8;410(3):614-9. PubMed PMID: 21684259.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Role of yeast JmjC-domain containing histone demethylases in actively transcribed regions. AU - Kwon,Dae-Whan, AU - Ahn,Seong Hoon, Y1 - 2011/06/12/ PY - 2011/05/19/received PY - 2011/06/06/accepted PY - 2011/6/21/entrez PY - 2011/6/21/pubmed PY - 2011/9/17/medline SP - 614 EP - 9 JF - Biochemical and biophysical research communications JO - Biochem. Biophys. Res. Commun. VL - 410 IS - 3 N2 - In budding yeast, there are five JmjC domain-containing proteins, Jhd1, Jhd2, Rph1, Ecm5, and Gis1, which have been suggested to directly remove histone lysine methylation via a hydroxylation reaction. Of these demethylases, the ability of Jhd1 or Rph1 to demethylate histone H3 as a substrate has been identified in vivo. However, the overall roles of endogenous JmjC demethylases in the demethylation of histones encompassed by genes that are constitutively transcribed or their specificities towards histone H3 lysine modification at mono-, di-, or trimethylation states are still unclear. Using chromatin immunoprecipitation with nine specific antibodies directed against mono-, di-, or trimethylated histone H3 at lysines 4, 36, or 79, we show the whole patterns of histone H3 lysine methylation and the net changes in methylations that are caused by the deletion of each of the five JmjC demethylases in actively transcribed regions. Our results show that of the JmjC-containing proteins, Rph1 is the demethylase that is specific for histone H3K36 trimethylation during transcription elongation in vivo, and the abilities of other endogenous JmjC demethylasesto demethylate histone H3 are weak toward histone H3in actively transcribed regions. SN - 1090-2104 UR - https://www.unboundmedicine.com/medline/citation/21684259/Role_of_yeast_JmjC_domain_containing_histone_demethylases_in_actively_transcribed_regions_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(11)00997-1 DB - PRIME DP - Unbound Medicine ER -