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[Sperm-oocyte interaction and in vitro fertilization clinical outcomes in patients with unexplained infertility].

Abstract

OBJECTIVE

To determine the sperm-zona pellucida (ZP) binding and ZP-induced acrosome reaction in patients with unexplained infertility, and to discuss the relationship between ZP-induced acrosome reaction and fertilization rate.

METHODS

We compared the fertilization rate and good embryo rate in patients with unexplained infertility after fertilization in 2 ways. Based on the causes of infertility, patients were divided into an unexplained infertility group (Group A) and a pure female tubal factor group (Group B). Oocytes which were obtained by super ovulation from 25 patients with unexplained infertility were randomly divided into 2 groups with conventional in vitro fertilization (IVF) (Group A1) and intracytoplasmic sperm injection (ICSI) fertilization (Group A2). The pure female tubal factor group (Group B) had conventional IVF. We conducted sperm-ZP binding and ZP-induced acrosome reaction experiments with 2 groups of men's sperms separately. We compared the number of sperm-egg binding and ZP-induced acrosome reaction rate and discussed the relationship between the ZP-induced acrosome reaction and fertilization rate, and also the fertilization rate, good embryo rate and pregnancy rate in patients with unexplained infertility after fertilization in 2 ways.

RESULTS

The average number of sperm-egg binding (78.29 ± 16.31) and the ZP-induced acrosome reaction rate (55.87 ± 27.69) % in Group A were lower than those of Group B [94.63 ± 6.72, (82.53 ± 17.99)%]. The difference between the average number of sperm-egg binding and the ZP-induced acrosome reaction was significant (P <0.01). The fertilization rate of Group A1 was significantly lower than that of Group B and Group A2 (P <0.01). But there was no significant difference in the good embryo rate among the 3 groups. There was no significant difference between Group A2 and B in fertilization rate and good embryo rate (P <0.05). There was no significant difference in pregnancy rate between Group A and B (P <0.05). Fertilization rate and the rate of acrosome reaction had marked positive correlation with statistical significance (r =0.932, P <0.01).

CONCLUSION

ZP binding and ZP-induced acrosome reaction are very important experiments in sperm function test for patients with unexplained infertility. It can not only effectively avoid no embryo transferring due to complete failure of fertilization but also get a desirable outcome of pregnancy using half-ICSI fertilization in patients with unexplained infertility.

Authors+Show Affiliations

Reproductive Medical Center, Department of Obstetrics and Gynaecology, Xiangya Hospital, Central South University, Changsha 410008, China. liunenghui@126.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

21685701

Citation

Liu, Nenghui, et al. "[Sperm-oocyte Interaction and in Vitro Fertilization Clinical Outcomes in Patients With Unexplained Infertility]." Zhong Nan Da Xue Xue Bao. Yi Xue Ban = Journal of Central South University. Medical Sciences, vol. 36, no. 5, 2011, pp. 439-47.
Liu N, Zhang Z, Li Y, et al. [Sperm-oocyte interaction and in vitro fertilization clinical outcomes in patients with unexplained infertility]. Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2011;36(5):439-47.
Liu, N., Zhang, Z., Li, Y., Liu, D., Chen, X., & Yao, Z. (2011). [Sperm-oocyte interaction and in vitro fertilization clinical outcomes in patients with unexplained infertility]. Zhong Nan Da Xue Xue Bao. Yi Xue Ban = Journal of Central South University. Medical Sciences, 36(5), pp. 439-47. doi:10.3969/j.issn.1672-7347.2011.05.012.
Liu N, et al. [Sperm-oocyte Interaction and in Vitro Fertilization Clinical Outcomes in Patients With Unexplained Infertility]. Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2011;36(5):439-47. PubMed PMID: 21685701.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Sperm-oocyte interaction and in vitro fertilization clinical outcomes in patients with unexplained infertility]. AU - Liu,Nenghui, AU - Zhang,Zhenhui, AU - Li,Yanping, AU - Liu,Dong'e, AU - Chen,Xianhua, AU - Yao,Zhongyuan, PY - 2011/6/21/entrez PY - 2011/6/21/pubmed PY - 2012/9/7/medline SP - 439 EP - 47 JF - Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences JO - Zhong Nan Da Xue Xue Bao Yi Xue Ban VL - 36 IS - 5 N2 - OBJECTIVE: To determine the sperm-zona pellucida (ZP) binding and ZP-induced acrosome reaction in patients with unexplained infertility, and to discuss the relationship between ZP-induced acrosome reaction and fertilization rate. METHODS: We compared the fertilization rate and good embryo rate in patients with unexplained infertility after fertilization in 2 ways. Based on the causes of infertility, patients were divided into an unexplained infertility group (Group A) and a pure female tubal factor group (Group B). Oocytes which were obtained by super ovulation from 25 patients with unexplained infertility were randomly divided into 2 groups with conventional in vitro fertilization (IVF) (Group A1) and intracytoplasmic sperm injection (ICSI) fertilization (Group A2). The pure female tubal factor group (Group B) had conventional IVF. We conducted sperm-ZP binding and ZP-induced acrosome reaction experiments with 2 groups of men's sperms separately. We compared the number of sperm-egg binding and ZP-induced acrosome reaction rate and discussed the relationship between the ZP-induced acrosome reaction and fertilization rate, and also the fertilization rate, good embryo rate and pregnancy rate in patients with unexplained infertility after fertilization in 2 ways. RESULTS: The average number of sperm-egg binding (78.29 ± 16.31) and the ZP-induced acrosome reaction rate (55.87 ± 27.69) % in Group A were lower than those of Group B [94.63 ± 6.72, (82.53 ± 17.99)%]. The difference between the average number of sperm-egg binding and the ZP-induced acrosome reaction was significant (P <0.01). The fertilization rate of Group A1 was significantly lower than that of Group B and Group A2 (P <0.01). But there was no significant difference in the good embryo rate among the 3 groups. There was no significant difference between Group A2 and B in fertilization rate and good embryo rate (P <0.05). There was no significant difference in pregnancy rate between Group A and B (P <0.05). Fertilization rate and the rate of acrosome reaction had marked positive correlation with statistical significance (r =0.932, P <0.01). CONCLUSION: ZP binding and ZP-induced acrosome reaction are very important experiments in sperm function test for patients with unexplained infertility. It can not only effectively avoid no embryo transferring due to complete failure of fertilization but also get a desirable outcome of pregnancy using half-ICSI fertilization in patients with unexplained infertility. SN - 1672-7347 UR - https://www.unboundmedicine.com/medline/citation/21685701/[Sperm_oocyte_interaction_and_in_vitro_fertilization_clinical_outcomes_in_patients_with_unexplained_infertility]_ L2 - http://xbyxb.csu.edu.cn/xbwk/fileup/PDF/201105439.pdf DB - PRIME DP - Unbound Medicine ER -