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Genotypic diversity of Streptococcus mutans and Streptococcus sobrinus in 3-4-year-old children with severe caries or without caries.
Int J Paediatr Dent. 2011 Nov; 21(6):422-31.IJ

Abstract

BACKGROUND

. The genotypic diversity of both Streptococcus mutans and Streptococcus sobrinus in children with different caries experience remains unclear. AIM. To investigate the genotypic diversity of S. mutans and S. sobrinus in children with severe early childhood caries (SECC) and in caries-free (CF) children.

METHODS.

Stimulated saliva of 87 SECC and 91 CF children aged 3-4 years was collected and submitted to cultivation, and MS colonies were enumerated. The genomic fingerprint analysis of S. mutans and S. sobrinus was carried out using AP-PCR.

RESULTS.

One to five genotypes of S. mutans were colonized in an oral cavity of SECC and CF children; 85.5% SECC children and 57.9% CF children harboured more than one genotype of S. mutans. One to three genotypes of S. sobrinus were detected from each SECC child; 31.25% SECC children harboured more than one genotype of S. sobrinus. And one genotype was colonized in each CF child. S. mutans isolates from different individuals displayed distinctive DNA fingerprints.

CONCLUSIONS.

DNA fingerprints of S. mutans and S. sobrinus isolates from 3- to 4-year-old children displayed genetic polymorphism, and S. mutans has greater genetic diversity than S. sobrinus. SECC children harboured more genotypes of S. mutans and S. sobrinus than CF children.

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Authors+Show Affiliations

Zhou Q
Department of Pediatric Dentistry, Peking University School of Stomatology, Beijing, China.
Qin X
No affiliation info available
Qin M
No affiliation info available
Ge L
No affiliation info available

MeSH

Case-Control StudiesChild, PreschoolColony Count, MicrobialDNA FingerprintingDNA, BacterialDental CariesGenetic VariationHumansMicrobial ConsortiaMouthPolymorphism, GeneticReference ValuesSalivaSeverity of Illness IndexStreptococcus mutansStreptococcus sobrinus

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21689176

Citation

Zhou, Qiong, et al. "Genotypic Diversity of Streptococcus Mutans and Streptococcus Sobrinus in 3-4-year-old Children With Severe Caries or Without Caries." International Journal of Paediatric Dentistry, vol. 21, no. 6, 2011, pp. 422-31.
Zhou Q, Qin X, Qin M, et al. Genotypic diversity of Streptococcus mutans and Streptococcus sobrinus in 3-4-year-old children with severe caries or without caries. Int J Paediatr Dent. 2011;21(6):422-31.
Zhou, Q., Qin, X., Qin, M., & Ge, L. (2011). Genotypic diversity of Streptococcus mutans and Streptococcus sobrinus in 3-4-year-old children with severe caries or without caries. International Journal of Paediatric Dentistry, 21(6), 422-31. https://doi.org/10.1111/j.1365-263X.2011.01145.x
Zhou Q, et al. Genotypic Diversity of Streptococcus Mutans and Streptococcus Sobrinus in 3-4-year-old Children With Severe Caries or Without Caries. Int J Paediatr Dent. 2011;21(6):422-31. PubMed PMID: 21689176.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genotypic diversity of Streptococcus mutans and Streptococcus sobrinus in 3-4-year-old children with severe caries or without caries. AU - Zhou,Qiong, AU - Qin,Xiurong, AU - Qin,Man, AU - Ge,Lihong, Y1 - 2011/06/20/ PY - 2011/6/22/entrez PY - 2011/6/22/pubmed PY - 2012/3/16/medline SP - 422 EP - 31 JF - International journal of paediatric dentistry JO - Int J Paediatr Dent VL - 21 IS - 6 N2 - BACKGROUND. The genotypic diversity of both Streptococcus mutans and Streptococcus sobrinus in children with different caries experience remains unclear. AIM. To investigate the genotypic diversity of S. mutans and S. sobrinus in children with severe early childhood caries (SECC) and in caries-free (CF) children. METHODS. Stimulated saliva of 87 SECC and 91 CF children aged 3-4 years was collected and submitted to cultivation, and MS colonies were enumerated. The genomic fingerprint analysis of S. mutans and S. sobrinus was carried out using AP-PCR. RESULTS. One to five genotypes of S. mutans were colonized in an oral cavity of SECC and CF children; 85.5% SECC children and 57.9% CF children harboured more than one genotype of S. mutans. One to three genotypes of S. sobrinus were detected from each SECC child; 31.25% SECC children harboured more than one genotype of S. sobrinus. And one genotype was colonized in each CF child. S. mutans isolates from different individuals displayed distinctive DNA fingerprints. CONCLUSIONS. DNA fingerprints of S. mutans and S. sobrinus isolates from 3- to 4-year-old children displayed genetic polymorphism, and S. mutans has greater genetic diversity than S. sobrinus. SECC children harboured more genotypes of S. mutans and S. sobrinus than CF children. SN - 1365-263X UR - https://www.unboundmedicine.com/medline/citation/21689176/Genotypic_diversity_of_Streptococcus_mutans_and_Streptococcus_sobrinus_in_3_4_year_old_children_with_severe_caries_or_without_caries_ L2 - https://doi.org/10.1111/j.1365-263X.2011.01145.x DB - PRIME DP - Unbound Medicine ER -