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Dectin1 activation of β-(1-3)/(1-6)-D-glucan produces an anti-mastitis effect in rats.
Inflamm Res. 2011 Oct; 60(10):937-45.IR

Abstract

OBJECTIVE

A lipopolysaccharide (LPS)-induced mastitis model in rats was used to study the protective effect of β-glucan.

MATERIALS AND METHODS

On gestation day 10, β-glucan was administered to rats daily by gavage at either 0 (control), 0.1 mg/kg BW, 1 mg/kg BW and 10 mg/kg BW until parturition. LPS or pyrogen-free physiological saline was inoculated into the mammary gland 72 h after parturition and the rats were euthanized at 12 h post-infection.

RESULTS

LPS increased dectin1 and toll-like receptor 4 (TLR4) mRNA and protein expression significantly in mammary tissues. Tumor necrosis factor (TNF)-α, interleukin (IL)-1β, N-acetyl-β-D-glucosaminidase (NAGase), inducible nitric oxide synthase (iNOs) in mammary tissues and serum, and myeloperoxidase (MPO) in mammary tissues were significantly increased 12 h after LPS infusion. β-glucan administration could enhance dectin1 mRNA and protein expression while downregulating the expression of TLR4. Level of TNF-α, IL-1β in mammary tissues and serum, MPO, NAGase and iNOs activity in mammary tissues was decreased, but the level of IL-2 in serum was increased by β-glucan.

CONCLUSION

The results indicate that β-glucan may protect mammary tissue against LPS-induced rat acute mastitis.

Authors+Show Affiliations

College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21691936

Citation

Zhu, Wei, et al. "Dectin1 Activation of β-(1-3)/(1-6)-D-glucan Produces an Anti-mastitis Effect in Rats." Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.], vol. 60, no. 10, 2011, pp. 937-45.
Zhu W, Gu B, Miao J, et al. Dectin1 activation of β-(1-3)/(1-6)-D-glucan produces an anti-mastitis effect in rats. Inflamm Res. 2011;60(10):937-45.
Zhu, W., Gu, B., Miao, J., Lu, J., & Zou, S. (2011). Dectin1 activation of β-(1-3)/(1-6)-D-glucan produces an anti-mastitis effect in rats. Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.], 60(10), 937-45. https://doi.org/10.1007/s00011-011-0354-1
Zhu W, et al. Dectin1 Activation of β-(1-3)/(1-6)-D-glucan Produces an Anti-mastitis Effect in Rats. Inflamm Res. 2011;60(10):937-45. PubMed PMID: 21691936.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dectin1 activation of β-(1-3)/(1-6)-D-glucan produces an anti-mastitis effect in rats. AU - Zhu,Wei, AU - Gu,Beibei, AU - Miao,Jinfeng, AU - Lu,Jingye, AU - Zou,Sixiang, Y1 - 2011/06/21/ PY - 2010/10/10/received PY - 2011/06/06/accepted PY - 2011/05/16/revised PY - 2011/6/22/entrez PY - 2011/6/22/pubmed PY - 2012/1/6/medline SP - 937 EP - 45 JF - Inflammation research : official journal of the European Histamine Research Society ... [et al.] JO - Inflamm. Res. VL - 60 IS - 10 N2 - OBJECTIVE: A lipopolysaccharide (LPS)-induced mastitis model in rats was used to study the protective effect of β-glucan. MATERIALS AND METHODS: On gestation day 10, β-glucan was administered to rats daily by gavage at either 0 (control), 0.1 mg/kg BW, 1 mg/kg BW and 10 mg/kg BW until parturition. LPS or pyrogen-free physiological saline was inoculated into the mammary gland 72 h after parturition and the rats were euthanized at 12 h post-infection. RESULTS: LPS increased dectin1 and toll-like receptor 4 (TLR4) mRNA and protein expression significantly in mammary tissues. Tumor necrosis factor (TNF)-α, interleukin (IL)-1β, N-acetyl-β-D-glucosaminidase (NAGase), inducible nitric oxide synthase (iNOs) in mammary tissues and serum, and myeloperoxidase (MPO) in mammary tissues were significantly increased 12 h after LPS infusion. β-glucan administration could enhance dectin1 mRNA and protein expression while downregulating the expression of TLR4. Level of TNF-α, IL-1β in mammary tissues and serum, MPO, NAGase and iNOs activity in mammary tissues was decreased, but the level of IL-2 in serum was increased by β-glucan. CONCLUSION: The results indicate that β-glucan may protect mammary tissue against LPS-induced rat acute mastitis. SN - 1420-908X UR - https://www.unboundmedicine.com/medline/citation/21691936/Dectin1_activation_of_β__1_3_/_1_6__D_glucan_produces_an_anti_mastitis_effect_in_rats_ L2 - https://dx.doi.org/10.1007/s00011-011-0354-1 DB - PRIME DP - Unbound Medicine ER -