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[Effects of penetration therapy with scalp electroacupuncture on gene expressions of nerve growth factors in substantia nigra of rats with Parkinson's disease].
Zhongguo Zhen Jiu. 2011 May; 31(5):435-40.ZZ

Abstract

OBJECTIVE

To explore the protective mechanism of penetration therapy with scalp electroacupuncture on Parkinson's disease (PD).

METHODS

Thirty-six Wistar rats were randomly divided into a normal group, a sham operation group, a model group and a penetration therapy group, 9 rats in each group. The sham operation group was operated by micro-injection with normal saline in the left corpus striatum. The model group and penetration therapy group were generated by micro-injection with 6-hydroxydopamine in the left corpus striatum to prepare rotation model of PD. The penetration therapy group was treated by penetration therapy with scalp electroacupuncture through "Baihui" (GV 20) to "Taiyang" (EX-HN 5), once each day, 6 days for one course, altogether 2 courses, and there was no treatment in the other two groups. (1) Immunohistochemical method was used to test the morphology and count of positive cells of tyrosine hydroxylase (TH). (2) In situ hybridization histochemistry was applied to detect the mRNA expression of brain-derived neurotrophic factor (BDNF).

RESULTS

(1) The areal density (AD), numerical density (ND) and integrating optic density (IOD) of the positive neurons of TH in substantia nigra in the penetration therapy group were 0.065 +/- 0.011, 0.014 +/- 0.003 and 0.470 +/- 0.099, respectively, which were higher than 0.039 +/- 0.008, 0.008 +/- 0.002 and 0.266 +/- 0.065 in the model group (all P < 0.05). (2) The AD, ND and IOD of the mRNA expression of BDNF in substantia nigra in the penetration therapy group were 0.100 +/- 0.012, 0.014 +/- 0.003 and 1.158 +/- 0.130, respectively, which were higher than 0.047 +/- 0.012, 0.007 +/- 0.001 and 0.602 +/- 0.108 in the model group (all P < 0.05).

CONCLUSION

The penetration therapy with scalp electroacupuncture has a better protective effect on dopaminergic neurons in substantia nigra of rats with PD. The protective mechanism is related to the provocation of neural nourishment so as to improve the morphous of dopaminergic neurons and increase the number of dopaminergic neurons.

Authors+Show Affiliations

College of TCM, Hainan Medical College, Haikou 571101, China. qi_xiujie@163.comNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

21692295

Citation

Qi, Xiu-Jie, and Shun Wang. "[Effects of Penetration Therapy With Scalp Electroacupuncture On Gene Expressions of Nerve Growth Factors in Substantia Nigra of Rats With Parkinson's Disease]." Zhongguo Zhen Jiu = Chinese Acupuncture & Moxibustion, vol. 31, no. 5, 2011, pp. 435-40.
Qi XJ, Wang S. [Effects of penetration therapy with scalp electroacupuncture on gene expressions of nerve growth factors in substantia nigra of rats with Parkinson's disease]. Zhongguo Zhen Jiu. 2011;31(5):435-40.
Qi, X. J., & Wang, S. (2011). [Effects of penetration therapy with scalp electroacupuncture on gene expressions of nerve growth factors in substantia nigra of rats with Parkinson's disease]. Zhongguo Zhen Jiu = Chinese Acupuncture & Moxibustion, 31(5), 435-40.
Qi XJ, Wang S. [Effects of Penetration Therapy With Scalp Electroacupuncture On Gene Expressions of Nerve Growth Factors in Substantia Nigra of Rats With Parkinson's Disease]. Zhongguo Zhen Jiu. 2011;31(5):435-40. PubMed PMID: 21692295.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effects of penetration therapy with scalp electroacupuncture on gene expressions of nerve growth factors in substantia nigra of rats with Parkinson's disease]. AU - Qi,Xiu-Jie, AU - Wang,Shun, PY - 2011/6/23/entrez PY - 2011/6/23/pubmed PY - 2011/8/5/medline SP - 435 EP - 40 JF - Zhongguo zhen jiu = Chinese acupuncture & moxibustion JO - Zhongguo Zhen Jiu VL - 31 IS - 5 N2 - OBJECTIVE: To explore the protective mechanism of penetration therapy with scalp electroacupuncture on Parkinson's disease (PD). METHODS: Thirty-six Wistar rats were randomly divided into a normal group, a sham operation group, a model group and a penetration therapy group, 9 rats in each group. The sham operation group was operated by micro-injection with normal saline in the left corpus striatum. The model group and penetration therapy group were generated by micro-injection with 6-hydroxydopamine in the left corpus striatum to prepare rotation model of PD. The penetration therapy group was treated by penetration therapy with scalp electroacupuncture through "Baihui" (GV 20) to "Taiyang" (EX-HN 5), once each day, 6 days for one course, altogether 2 courses, and there was no treatment in the other two groups. (1) Immunohistochemical method was used to test the morphology and count of positive cells of tyrosine hydroxylase (TH). (2) In situ hybridization histochemistry was applied to detect the mRNA expression of brain-derived neurotrophic factor (BDNF). RESULTS: (1) The areal density (AD), numerical density (ND) and integrating optic density (IOD) of the positive neurons of TH in substantia nigra in the penetration therapy group were 0.065 +/- 0.011, 0.014 +/- 0.003 and 0.470 +/- 0.099, respectively, which were higher than 0.039 +/- 0.008, 0.008 +/- 0.002 and 0.266 +/- 0.065 in the model group (all P < 0.05). (2) The AD, ND and IOD of the mRNA expression of BDNF in substantia nigra in the penetration therapy group were 0.100 +/- 0.012, 0.014 +/- 0.003 and 1.158 +/- 0.130, respectively, which were higher than 0.047 +/- 0.012, 0.007 +/- 0.001 and 0.602 +/- 0.108 in the model group (all P < 0.05). CONCLUSION: The penetration therapy with scalp electroacupuncture has a better protective effect on dopaminergic neurons in substantia nigra of rats with PD. The protective mechanism is related to the provocation of neural nourishment so as to improve the morphous of dopaminergic neurons and increase the number of dopaminergic neurons. SN - 0255-2930 UR - https://www.unboundmedicine.com/medline/citation/21692295/[Effects_of_penetration_therapy_with_scalp_electroacupuncture_on_gene_expressions_of_nerve_growth_factors_in_substantia_nigra_of_rats_with_Parkinson's_disease]_ L2 - https://medlineplus.gov/parkinsonsdisease.html DB - PRIME DP - Unbound Medicine ER -