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Comparative study of antioxidant and anti-inflammatory activities and genotoxicity of alcoholic and aqueous extracts of four Fabiana species that grow in mountainous area of Argentina.
J Ethnopharmacol. 2011 Sep 01; 137(1):512-22.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Fabiana species (Solanaceae family) extracts have long been used in Argentinean traditional medicine as anti-inflammatories, antiseptic, bone fractures and others diseases, but there is no scientific evidence which supports their use.

AIM OF THE STUDY

The present study was conducted to evaluate the ability of aqueous and ethanolic extracts of four Fabiana species (Fabiana bryoides Phil., Fabiana punensis A.C. Arroyo, Fabiana densa J. Rèmy and Fabiana patagonica Speg.) to inhibit key enzymes in inflammatory processes, free radical scavenging properties and genotoxic effects.

MATERIALS AND METHODS

HPLC-DAD of aqueous and ethanolic extracts from four Fabiana species was established. All Fabiana extracts were evaluated on their ability to inhibit hyaluronidase and lipoxygenase enzymes to assess their activity against inflammatory mediators. Antioxidant capacity was determined using the 2,2'-azino-bis 3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and the 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging assays and β-carotene-linolenic acid assay. Genotoxicity was evaluated by the Ames assay.

RESULTS

The results indicated that the chromatographic patterns of four Fabiana species were different in quantity and absorption intensity of peaks. The alcoholic extract of Fabiana punensis was the most active scavenger of DPPH and ABTS(+) radicals (SC(50) values of 3.85 ± 0.24 and 2.56 ± 0.10 μgGAE/mL, respectively). Fabiana patagonica extracts exhibited the highest peroxyl radical scavenging activity compared with the other three taxa (IC(50) values between 1.00 ± 0.04 and 4.46 ± 0.40 μg GAE/mL for all extracts) and anti-lipoxygenase activity with IC(50) values between 12.5 and 15.5 μg GAE/mL. The absence of mutagenicity indicates that the DNA does not seem to be a relevant target for these extracts. Fabiana bryoides ethanolic extract showed an interesting effect: it inhibited spontaneous mutagenesis, which could be considered as an antimutagenic effect in the TA98 (+S9) and TA100 (+S9/-S9) strains. The potency differences found between the species could be consequence of the different phytochemical pattern observed by HPLC.

CONCLUSIONS

The inhibitory effects on lipoxygenase and hyaluronidase, free radical scavenging activities and lack of genotoxicity of Fabiana extracts may support the folk use of Fabiana punensis, Fabiana patagonica, Fabiana bryoides and Fabiana densa as inhibitor of inflammatory mediators.

Authors+Show Affiliations

INQUINOA (CONICET), Universidad Nacional de Tucumán, San Lorenzo 1469, 4000 San Miguel de Tucumán, Argentina.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21693175

Citation

Cuello, S, et al. "Comparative Study of Antioxidant and Anti-inflammatory Activities and Genotoxicity of Alcoholic and Aqueous Extracts of Four Fabiana Species That Grow in Mountainous Area of Argentina." Journal of Ethnopharmacology, vol. 137, no. 1, 2011, pp. 512-22.
Cuello S, Alberto MR, Zampini IC, et al. Comparative study of antioxidant and anti-inflammatory activities and genotoxicity of alcoholic and aqueous extracts of four Fabiana species that grow in mountainous area of Argentina. J Ethnopharmacol. 2011;137(1):512-22.
Cuello, S., Alberto, M. R., Zampini, I. C., Ordoñez, R. M., & Isla, M. I. (2011). Comparative study of antioxidant and anti-inflammatory activities and genotoxicity of alcoholic and aqueous extracts of four Fabiana species that grow in mountainous area of Argentina. Journal of Ethnopharmacology, 137(1), 512-22. https://doi.org/10.1016/j.jep.2011.06.005
Cuello S, et al. Comparative Study of Antioxidant and Anti-inflammatory Activities and Genotoxicity of Alcoholic and Aqueous Extracts of Four Fabiana Species That Grow in Mountainous Area of Argentina. J Ethnopharmacol. 2011 Sep 1;137(1):512-22. PubMed PMID: 21693175.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparative study of antioxidant and anti-inflammatory activities and genotoxicity of alcoholic and aqueous extracts of four Fabiana species that grow in mountainous area of Argentina. AU - Cuello,S, AU - Alberto,M R, AU - Zampini,I C, AU - Ordoñez,R M, AU - Isla,M I, Y1 - 2011/06/13/ PY - 2011/04/25/received PY - 2011/06/04/revised PY - 2011/06/05/accepted PY - 2011/6/23/entrez PY - 2011/6/23/pubmed PY - 2011/12/28/medline SP - 512 EP - 22 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 137 IS - 1 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Fabiana species (Solanaceae family) extracts have long been used in Argentinean traditional medicine as anti-inflammatories, antiseptic, bone fractures and others diseases, but there is no scientific evidence which supports their use. AIM OF THE STUDY: The present study was conducted to evaluate the ability of aqueous and ethanolic extracts of four Fabiana species (Fabiana bryoides Phil., Fabiana punensis A.C. Arroyo, Fabiana densa J. Rèmy and Fabiana patagonica Speg.) to inhibit key enzymes in inflammatory processes, free radical scavenging properties and genotoxic effects. MATERIALS AND METHODS: HPLC-DAD of aqueous and ethanolic extracts from four Fabiana species was established. All Fabiana extracts were evaluated on their ability to inhibit hyaluronidase and lipoxygenase enzymes to assess their activity against inflammatory mediators. Antioxidant capacity was determined using the 2,2'-azino-bis 3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and the 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging assays and β-carotene-linolenic acid assay. Genotoxicity was evaluated by the Ames assay. RESULTS: The results indicated that the chromatographic patterns of four Fabiana species were different in quantity and absorption intensity of peaks. The alcoholic extract of Fabiana punensis was the most active scavenger of DPPH and ABTS(+) radicals (SC(50) values of 3.85 ± 0.24 and 2.56 ± 0.10 μgGAE/mL, respectively). Fabiana patagonica extracts exhibited the highest peroxyl radical scavenging activity compared with the other three taxa (IC(50) values between 1.00 ± 0.04 and 4.46 ± 0.40 μg GAE/mL for all extracts) and anti-lipoxygenase activity with IC(50) values between 12.5 and 15.5 μg GAE/mL. The absence of mutagenicity indicates that the DNA does not seem to be a relevant target for these extracts. Fabiana bryoides ethanolic extract showed an interesting effect: it inhibited spontaneous mutagenesis, which could be considered as an antimutagenic effect in the TA98 (+S9) and TA100 (+S9/-S9) strains. The potency differences found between the species could be consequence of the different phytochemical pattern observed by HPLC. CONCLUSIONS: The inhibitory effects on lipoxygenase and hyaluronidase, free radical scavenging activities and lack of genotoxicity of Fabiana extracts may support the folk use of Fabiana punensis, Fabiana patagonica, Fabiana bryoides and Fabiana densa as inhibitor of inflammatory mediators. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/21693175/Comparative_study_of_antioxidant_and_anti_inflammatory_activities_and_genotoxicity_of_alcoholic_and_aqueous_extracts_of_four_Fabiana_species_that_grow_in_mountainous_area_of_Argentina_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(11)00422-3 DB - PRIME DP - Unbound Medicine ER -