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Usefulness of the restriction-modification test plus staphylococcal cassette chromosome mec types and Panton-Valentine leukocidin encoding phages to identify Staphylococcus aureus methicillin-resistant clones.
Scand J Infect Dis 2011; 43(11-12):943-6SJ

Abstract

We studied the usefulness of the restriction-modification (RM) test, staphylococcal cassette chromosome (SCC) mec types, and Panton-Valentine leukocidin (PVL)-encoding phages to identify Staphylococcus aureus methicillin-resistant lineages and to differentiate clones that belong to the same lineage. A total of 108 methicillin-resistant S. aureus (MRSA) strains were characterized by pulsed-field gel electrophoresis (PFGE)--multi-locus sequence typing (MLST)--spa-typing. The RM correctly identified the lineages CC5, CC8, CC30, and CC398, but not CC25 and CC72. The SCCmec and RM combined analysis allowed differentiation between MLST types within the same lineage. Only 5 MRSA strains were PVL-positive. Four PVL-positive USA300 isolates carried elongated-head type PVL-encoding phages, while the sequence type (ST)-30 strain carried an icosahedral-head phage. The combination of the RM test method, SCCmec types, and PVL phage identification could be useful for MRSA typing purposes.

Authors+Show Affiliations

Microbiology Laboratory, Complexo Hospitalario Universitario de Vigo, Vigo, Spain. carmen.potel.alvarellos@sergas.esNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21696251

Citation

Alvarellos, Carmen Potel, et al. "Usefulness of the Restriction-modification Test Plus Staphylococcal Cassette Chromosome Mec Types and Panton-Valentine Leukocidin Encoding Phages to Identify Staphylococcus Aureus Methicillin-resistant Clones." Scandinavian Journal of Infectious Diseases, vol. 43, no. 11-12, 2011, pp. 943-6.
Alvarellos CP, Carames LC, Castro SP, et al. Usefulness of the restriction-modification test plus staphylococcal cassette chromosome mec types and Panton-Valentine leukocidin encoding phages to identify Staphylococcus aureus methicillin-resistant clones. Scand J Infect Dis. 2011;43(11-12):943-6.
Alvarellos, C. P., Carames, L. C., Castro, S. P., Garcia, P. A., Pi On, J. T., & Fernandez, M. A. (2011). Usefulness of the restriction-modification test plus staphylococcal cassette chromosome mec types and Panton-Valentine leukocidin encoding phages to identify Staphylococcus aureus methicillin-resistant clones. Scandinavian Journal of Infectious Diseases, 43(11-12), pp. 943-6. doi:10.3109/00365548.2011.589078.
Alvarellos CP, et al. Usefulness of the Restriction-modification Test Plus Staphylococcal Cassette Chromosome Mec Types and Panton-Valentine Leukocidin Encoding Phages to Identify Staphylococcus Aureus Methicillin-resistant Clones. Scand J Infect Dis. 2011;43(11-12):943-6. PubMed PMID: 21696251.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Usefulness of the restriction-modification test plus staphylococcal cassette chromosome mec types and Panton-Valentine leukocidin encoding phages to identify Staphylococcus aureus methicillin-resistant clones. AU - Alvarellos,Carmen Potel, AU - Carames,Lucia Constenla, AU - Castro,Sonia Perez, AU - Garcia,Patricia Alvarez, AU - Pi On,Julio Torres, AU - Fernandez,Maximiliano Alvarez, Y1 - 2011/06/22/ PY - 2011/6/24/entrez PY - 2011/6/24/pubmed PY - 2012/2/22/medline SP - 943 EP - 6 JF - Scandinavian journal of infectious diseases JO - Scand. J. Infect. Dis. VL - 43 IS - 11-12 N2 - We studied the usefulness of the restriction-modification (RM) test, staphylococcal cassette chromosome (SCC) mec types, and Panton-Valentine leukocidin (PVL)-encoding phages to identify Staphylococcus aureus methicillin-resistant lineages and to differentiate clones that belong to the same lineage. A total of 108 methicillin-resistant S. aureus (MRSA) strains were characterized by pulsed-field gel electrophoresis (PFGE)--multi-locus sequence typing (MLST)--spa-typing. The RM correctly identified the lineages CC5, CC8, CC30, and CC398, but not CC25 and CC72. The SCCmec and RM combined analysis allowed differentiation between MLST types within the same lineage. Only 5 MRSA strains were PVL-positive. Four PVL-positive USA300 isolates carried elongated-head type PVL-encoding phages, while the sequence type (ST)-30 strain carried an icosahedral-head phage. The combination of the RM test method, SCCmec types, and PVL phage identification could be useful for MRSA typing purposes. SN - 1651-1980 UR - https://www.unboundmedicine.com/medline/citation/21696251/Usefulness_of_the_restriction_modification_test_plus_staphylococcal_cassette_chromosome_mec_types_and_Panton_Valentine_leukocidin_encoding_phages_to_identify_Staphylococcus_aureus_methicillin_resistant_clones_ L2 - http://www.tandfonline.com/doi/full/10.3109/00365548.2011.589078 DB - PRIME DP - Unbound Medicine ER -