Tags

Type your tag names separated by a space and hit enter

In-situ produced ascorbic acid as coreactant for an ultrasensitive solid-state tris(2,2'-bipyridyl) ruthenium(II) electrochemiluminescence aptasensor.
Biosens Bioelectron. 2011 Aug 15; 26(12):4815-8.BB

Abstract

Herein, an ultrasensitive solid-state tris(2,2'-bipyridyl) ruthenium(II) (Ru(bpy)(3)(2+)) electrochemiluminescence (ECL) aptasensor using in-situ produced ascorbic acid as coreactant was successfully constructed for detection of thrombin. Firstly, the composite of Ru(bpy)(3)(2+) and platinum nanoparticles (Ru-PtNPs) were immobilized onto Nafion coated glass carbon electrode, followed by successive adsorption of streptavidin-alkaine phosphatase conjugate (SA-ALP) and biotinylated anti-thrombin aptamer to successfully construct an ECL aptasensor for thrombin determination. In our design, Pt nanoparticles in Ru(bpy)(3)(2+)-Nafion film successfully inhibited the migration of Ru(bpy)(3)(2+) into the electrochemically hydrophobic region of Nafion and facilitated the electron transfer between Ru(bpy)(3)(2+) and electrode surface. Furthermore, ALP on the electrode surface could catalyze hydrolysis of ascorbic acid 2-phosphate to in-situ produce ascorbic acid, which co-reacted with Ru(bpy)(3)(2+) to obtain quite fast, stable and greatly amplified ECL signal. The experimental results indicated that the aptasensor exhibited good response for thrombin with excellent sensitivity, selectivity and stability. A linear range of 1 × 10(-15)-1 × 10(-8) M with an ultralow detection limit of 0.33 fM (S/N=3) was obtained. Thus, this procedure has great promise for detection of thrombin present at ultra-trace levels during early stage of diseases.

Authors+Show Affiliations

Education Ministry Key Laboratory on Luminescence and Real-Time Analysis, College of Chemistry and Chemical Engineering, Southwest University, No. 2 Tiansheng Road, Chongqing 400715, People's Republic of China. yuhong87@swu.edu.cnNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21696941

Citation

Liao, Yuhong, et al. "In-situ Produced Ascorbic Acid as Coreactant for an Ultrasensitive Solid-state Tris(2,2'-bipyridyl) ruthenium(II) Electrochemiluminescence Aptasensor." Biosensors & Bioelectronics, vol. 26, no. 12, 2011, pp. 4815-8.
Liao Y, Yuan R, Chai Y, et al. In-situ produced ascorbic acid as coreactant for an ultrasensitive solid-state tris(2,2'-bipyridyl) ruthenium(II) electrochemiluminescence aptasensor. Biosens Bioelectron. 2011;26(12):4815-8.
Liao, Y., Yuan, R., Chai, Y., Zhuo, Y., Yuan, Y., Bai, L., Mao, L., & Yuan, S. (2011). In-situ produced ascorbic acid as coreactant for an ultrasensitive solid-state tris(2,2'-bipyridyl) ruthenium(II) electrochemiluminescence aptasensor. Biosensors & Bioelectronics, 26(12), 4815-8. https://doi.org/10.1016/j.bios.2011.04.019
Liao Y, et al. In-situ Produced Ascorbic Acid as Coreactant for an Ultrasensitive Solid-state Tris(2,2'-bipyridyl) ruthenium(II) Electrochemiluminescence Aptasensor. Biosens Bioelectron. 2011 Aug 15;26(12):4815-8. PubMed PMID: 21696941.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In-situ produced ascorbic acid as coreactant for an ultrasensitive solid-state tris(2,2'-bipyridyl) ruthenium(II) electrochemiluminescence aptasensor. AU - Liao,Yuhong, AU - Yuan,Ruo, AU - Chai,Yaqin, AU - Zhuo,Ying, AU - Yuan,Yali, AU - Bai,Lijuan, AU - Mao,Li, AU - Yuan,Shirong, Y1 - 2011/04/28/ PY - 2011/01/24/received PY - 2011/04/11/revised PY - 2011/04/11/accepted PY - 2011/6/24/entrez PY - 2011/6/24/pubmed PY - 2011/12/13/medline SP - 4815 EP - 8 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 26 IS - 12 N2 - Herein, an ultrasensitive solid-state tris(2,2'-bipyridyl) ruthenium(II) (Ru(bpy)(3)(2+)) electrochemiluminescence (ECL) aptasensor using in-situ produced ascorbic acid as coreactant was successfully constructed for detection of thrombin. Firstly, the composite of Ru(bpy)(3)(2+) and platinum nanoparticles (Ru-PtNPs) were immobilized onto Nafion coated glass carbon electrode, followed by successive adsorption of streptavidin-alkaine phosphatase conjugate (SA-ALP) and biotinylated anti-thrombin aptamer to successfully construct an ECL aptasensor for thrombin determination. In our design, Pt nanoparticles in Ru(bpy)(3)(2+)-Nafion film successfully inhibited the migration of Ru(bpy)(3)(2+) into the electrochemically hydrophobic region of Nafion and facilitated the electron transfer between Ru(bpy)(3)(2+) and electrode surface. Furthermore, ALP on the electrode surface could catalyze hydrolysis of ascorbic acid 2-phosphate to in-situ produce ascorbic acid, which co-reacted with Ru(bpy)(3)(2+) to obtain quite fast, stable and greatly amplified ECL signal. The experimental results indicated that the aptasensor exhibited good response for thrombin with excellent sensitivity, selectivity and stability. A linear range of 1 × 10(-15)-1 × 10(-8) M with an ultralow detection limit of 0.33 fM (S/N=3) was obtained. Thus, this procedure has great promise for detection of thrombin present at ultra-trace levels during early stage of diseases. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/21696941/In_situ_produced_ascorbic_acid_as_coreactant_for_an_ultrasensitive_solid_state_tris_22'_bipyridyl__ruthenium_II__electrochemiluminescence_aptasensor_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(11)00216-8 DB - PRIME DP - Unbound Medicine ER -