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Effects of NADH-preferring xylose reductase expression on ethanol production from xylose in xylose-metabolizing recombinant Saccharomyces cerevisiae.
J Biotechnol. 2012 Apr 30; 158(4):184-91.JB

Abstract

Efficient conversion of xylose to ethanol is an essential factor for commercialization of lignocellulosic ethanol. To minimize production of xylitol, a major by-product in xylose metabolism and concomitantly improve ethanol production, Saccharomyces cerevisiae D452-2 was engineered to overexpress NADH-preferable xylose reductase mutant (XR(MUT)) and NAD⁺-dependent xylitol dehydrogenase (XDH) from Pichia stipitis and endogenous xylulokinase (XK). In vitro enzyme assay confirmed the functional expression of XR(MUT), XDH and XK in recombinant S. cerevisiae strains. The change of wild type XR to XR(MUT) along with XK overexpression led to reduction of xylitol accumulation in microaerobic culture. More modulation of the xylose metabolism including overexpression of XR(MUT) and transaldolase, and disruption of the chromosomal ALD6 gene encoding aldehyde dehydrogenase (SX6(MUT)) improved the performance of ethanol production from xylose remarkably. Finally, oxygen-limited fermentation of S. cerevisiae SX6(MUT) resulted in 0.64 g l⁻¹ h⁻¹ xylose consumption rate, 0.25 g l⁻¹ h⁻¹ ethanol productivity and 39% ethanol yield based on the xylose consumed, which were 1.8, 4.2 and 2.2 times higher than the corresponding values of recombinant S. cerevisiae expressing XR(MUT), XDH and XK only.

Authors+Show Affiliations

Interdisciplinary Program for Bioengineering, Seoul National University, Seoul 151-742, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21699927

Citation

Lee, Sung-Haeng, et al. "Effects of NADH-preferring Xylose Reductase Expression On Ethanol Production From Xylose in Xylose-metabolizing Recombinant Saccharomyces Cerevisiae." Journal of Biotechnology, vol. 158, no. 4, 2012, pp. 184-91.
Lee SH, Kodaki T, Park YC, et al. Effects of NADH-preferring xylose reductase expression on ethanol production from xylose in xylose-metabolizing recombinant Saccharomyces cerevisiae. J Biotechnol. 2012;158(4):184-91.
Lee, S. H., Kodaki, T., Park, Y. C., & Seo, J. H. (2012). Effects of NADH-preferring xylose reductase expression on ethanol production from xylose in xylose-metabolizing recombinant Saccharomyces cerevisiae. Journal of Biotechnology, 158(4), 184-91. https://doi.org/10.1016/j.jbiotec.2011.06.005
Lee SH, et al. Effects of NADH-preferring Xylose Reductase Expression On Ethanol Production From Xylose in Xylose-metabolizing Recombinant Saccharomyces Cerevisiae. J Biotechnol. 2012 Apr 30;158(4):184-91. PubMed PMID: 21699927.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of NADH-preferring xylose reductase expression on ethanol production from xylose in xylose-metabolizing recombinant Saccharomyces cerevisiae. AU - Lee,Sung-Haeng, AU - Kodaki,Tsutomu, AU - Park,Yong-Cheol, AU - Seo,Jin-Ho, Y1 - 2011/06/15/ PY - 2011/01/31/received PY - 2011/05/26/revised PY - 2011/06/07/accepted PY - 2011/6/25/entrez PY - 2011/6/28/pubmed PY - 2012/10/17/medline SP - 184 EP - 91 JF - Journal of biotechnology JO - J Biotechnol VL - 158 IS - 4 N2 - Efficient conversion of xylose to ethanol is an essential factor for commercialization of lignocellulosic ethanol. To minimize production of xylitol, a major by-product in xylose metabolism and concomitantly improve ethanol production, Saccharomyces cerevisiae D452-2 was engineered to overexpress NADH-preferable xylose reductase mutant (XR(MUT)) and NAD⁺-dependent xylitol dehydrogenase (XDH) from Pichia stipitis and endogenous xylulokinase (XK). In vitro enzyme assay confirmed the functional expression of XR(MUT), XDH and XK in recombinant S. cerevisiae strains. The change of wild type XR to XR(MUT) along with XK overexpression led to reduction of xylitol accumulation in microaerobic culture. More modulation of the xylose metabolism including overexpression of XR(MUT) and transaldolase, and disruption of the chromosomal ALD6 gene encoding aldehyde dehydrogenase (SX6(MUT)) improved the performance of ethanol production from xylose remarkably. Finally, oxygen-limited fermentation of S. cerevisiae SX6(MUT) resulted in 0.64 g l⁻¹ h⁻¹ xylose consumption rate, 0.25 g l⁻¹ h⁻¹ ethanol productivity and 39% ethanol yield based on the xylose consumed, which were 1.8, 4.2 and 2.2 times higher than the corresponding values of recombinant S. cerevisiae expressing XR(MUT), XDH and XK only. SN - 1873-4863 UR - https://www.unboundmedicine.com/medline/citation/21699927/Effects_of_NADH_preferring_xylose_reductase_expression_on_ethanol_production_from_xylose_in_xylose_metabolizing_recombinant_Saccharomyces_cerevisiae_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0168-1656(11)00304-X DB - PRIME DP - Unbound Medicine ER -