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The secD locus of E.coli codes for two membrane proteins required for protein export.
EMBO J 1990; 9(10):3209-16EJ

Abstract

Cold-sensitive mutations in the secD locus of Escherichia coli result in severe defects in protein export at the non-permissive temperature of 23 degrees C. DNA sequence of a cloned fragment that includes the secD locus reveals open reading frames for seven polypeptide chains. Both deletions and TnphoA insertions in this clone have been used in maxicell and complementation studies to define the secD locus and its products. The secD mutations fall into two complementation groups, defining genes we have named secD and secF. These two genes comprise an operon, the first case of two genes involved in the export process being co-transcribed. The DNA sequence of the two genes along with alkaline phosphatase fusion analysis indicates that they code for integral proteins of the cytoplasmic membrane. We suggest that these two proteins may form a complex in the membrane which acts at late steps in the export process.

Authors+Show Affiliations

Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

2170107

Citation

Gardel, C, et al. "The secD Locus of E.coli Codes for Two Membrane Proteins Required for Protein Export." The EMBO Journal, vol. 9, no. 10, 1990, pp. 3209-16.
Gardel C, Johnson K, Jacq A, et al. The secD locus of E.coli codes for two membrane proteins required for protein export. EMBO J. 1990;9(10):3209-16.
Gardel, C., Johnson, K., Jacq, A., & Beckwith, J. (1990). The secD locus of E.coli codes for two membrane proteins required for protein export. The EMBO Journal, 9(10), pp. 3209-16.
Gardel C, et al. The secD Locus of E.coli Codes for Two Membrane Proteins Required for Protein Export. EMBO J. 1990;9(10):3209-16. PubMed PMID: 2170107.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The secD locus of E.coli codes for two membrane proteins required for protein export. AU - Gardel,C, AU - Johnson,K, AU - Jacq,A, AU - Beckwith,J, PY - 1990/10/1/pubmed PY - 1990/10/1/medline PY - 1990/10/1/entrez SP - 3209 EP - 16 JF - The EMBO journal JO - EMBO J. VL - 9 IS - 10 N2 - Cold-sensitive mutations in the secD locus of Escherichia coli result in severe defects in protein export at the non-permissive temperature of 23 degrees C. DNA sequence of a cloned fragment that includes the secD locus reveals open reading frames for seven polypeptide chains. Both deletions and TnphoA insertions in this clone have been used in maxicell and complementation studies to define the secD locus and its products. The secD mutations fall into two complementation groups, defining genes we have named secD and secF. These two genes comprise an operon, the first case of two genes involved in the export process being co-transcribed. The DNA sequence of the two genes along with alkaline phosphatase fusion analysis indicates that they code for integral proteins of the cytoplasmic membrane. We suggest that these two proteins may form a complex in the membrane which acts at late steps in the export process. SN - 0261-4189 UR - https://www.unboundmedicine.com/medline/citation/2170107/The_secD_locus_of_E_coli_codes_for_two_membrane_proteins_required_for_protein_export_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/2170107/ DB - PRIME DP - Unbound Medicine ER -