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Alteration of sodium, potassium-adenosine triphosphatase activity in rabbit ciliary processes by cyclic adenosine monophosphate-dependent protein kinase.
Invest Ophthalmol Vis Sci. 1990 Oct; 31(10):2164-70.IO

Abstract

The response of sodium, potassium-adenosine triphosphatase (Na,K-ATPase) to cyclic adenosine monophosphate (cAMP)-dependent protein kinase was examined in membranes obtained from rabbit iris-ciliary body. In the presence of the protein kinase together with 10(-5) M cAMP, Na,K-ATPase activity was reduced. No change in Na,K-ATPase activity was detected in response to the protein kinase without added cAMP. Likewise cAMP alone did not alter Na,K-ATPase activity. Reduction of Na,K-ATPase activity was also observed in the presence of the cAMP-dependent protein kinase catalytic subunit. The response of the enzyme to the kinase catalytic subunit was also examined in membranes obtained from rabbit ciliary processes. In the presence of 8 micrograms/ml of the catalytic subunit, ciliary process Na,K-ATPase activity was reduced by more than 50%. To examine whether other ATPases were suppressed by the protein kinase, calcium-stimulated ATPase activity was examined; its activity was stimulated by the catalytic subunit. To test whether the response of the ciliary process Na,K-ATPase is unique, experiments were also performed using membrane preparations from rabbit lens epithelium or rabbit kidney; the catalytic subunit significantly reduced the activity of Na,K-ATPase from the kidney but not the lens. These Na,K-ATPase studies suggest that in the iris-ciliary body, cAMP may alter sodium pump activity. In parallel 86Rb uptake studies, we observed that ouabain-inhibitable potassium uptake by intact pieces of iris-ciliary body was reduced by exogenous dibutryl cAMP or by forskolin.

Authors+Show Affiliations

Department of Ophthalmology and Visual Sciences, University of Louisville School of Medicine, Kentucky.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

2170291

Citation

Delamere, N A., et al. "Alteration of Sodium, Potassium-adenosine Triphosphatase Activity in Rabbit Ciliary Processes By Cyclic Adenosine Monophosphate-dependent Protein Kinase." Investigative Ophthalmology & Visual Science, vol. 31, no. 10, 1990, pp. 2164-70.
Delamere NA, Socci RR, King KL. Alteration of sodium, potassium-adenosine triphosphatase activity in rabbit ciliary processes by cyclic adenosine monophosphate-dependent protein kinase. Invest Ophthalmol Vis Sci. 1990;31(10):2164-70.
Delamere, N. A., Socci, R. R., & King, K. L. (1990). Alteration of sodium, potassium-adenosine triphosphatase activity in rabbit ciliary processes by cyclic adenosine monophosphate-dependent protein kinase. Investigative Ophthalmology & Visual Science, 31(10), 2164-70.
Delamere NA, Socci RR, King KL. Alteration of Sodium, Potassium-adenosine Triphosphatase Activity in Rabbit Ciliary Processes By Cyclic Adenosine Monophosphate-dependent Protein Kinase. Invest Ophthalmol Vis Sci. 1990;31(10):2164-70. PubMed PMID: 2170291.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Alteration of sodium, potassium-adenosine triphosphatase activity in rabbit ciliary processes by cyclic adenosine monophosphate-dependent protein kinase. AU - Delamere,N A, AU - Socci,R R, AU - King,K L, PY - 1990/10/1/pubmed PY - 1990/10/1/medline PY - 1990/10/1/entrez SP - 2164 EP - 70 JF - Investigative ophthalmology & visual science JO - Invest Ophthalmol Vis Sci VL - 31 IS - 10 N2 - The response of sodium, potassium-adenosine triphosphatase (Na,K-ATPase) to cyclic adenosine monophosphate (cAMP)-dependent protein kinase was examined in membranes obtained from rabbit iris-ciliary body. In the presence of the protein kinase together with 10(-5) M cAMP, Na,K-ATPase activity was reduced. No change in Na,K-ATPase activity was detected in response to the protein kinase without added cAMP. Likewise cAMP alone did not alter Na,K-ATPase activity. Reduction of Na,K-ATPase activity was also observed in the presence of the cAMP-dependent protein kinase catalytic subunit. The response of the enzyme to the kinase catalytic subunit was also examined in membranes obtained from rabbit ciliary processes. In the presence of 8 micrograms/ml of the catalytic subunit, ciliary process Na,K-ATPase activity was reduced by more than 50%. To examine whether other ATPases were suppressed by the protein kinase, calcium-stimulated ATPase activity was examined; its activity was stimulated by the catalytic subunit. To test whether the response of the ciliary process Na,K-ATPase is unique, experiments were also performed using membrane preparations from rabbit lens epithelium or rabbit kidney; the catalytic subunit significantly reduced the activity of Na,K-ATPase from the kidney but not the lens. These Na,K-ATPase studies suggest that in the iris-ciliary body, cAMP may alter sodium pump activity. In parallel 86Rb uptake studies, we observed that ouabain-inhibitable potassium uptake by intact pieces of iris-ciliary body was reduced by exogenous dibutryl cAMP or by forskolin. SN - 0146-0404 UR - https://www.unboundmedicine.com/medline/citation/2170291/Alteration_of_sodium_potassium_adenosine_triphosphatase_activity_in_rabbit_ciliary_processes_by_cyclic_adenosine_monophosphate_dependent_protein_kinase_ L2 - https://iovs.arvojournals.org/article.aspx?volume=31&issue=10&page=2164 DB - PRIME DP - Unbound Medicine ER -