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Evaluation of rapid immunochromatographic NS1 test, anti-dengue IgM test, semi-nested PCR and IgM ELISA for detection of dengue virus.

Abstract

Dengue virus (DENV) causes various clinical symptoms of differing severity based on time of infections. The existing laboratory methods, semi-nested PCR and Dengue IgM ELISA, still have limitations for diagnosis. A commercially available rapid immunochromatographic dengue NS1 antigen and IgM antibody tests in comparison with semi-nested PCR and IgM ELISA for confirmation of DENV infection were evaluated. In total, 237 single acute serum specimens and 50 paired sera of dengue patients were examined using the rapid dengue NS1 antigen test, IgM antibody test, semi-nested PCR and Dengue IgM ELISA. The NS1 and IgM rapid tests showed sensitivity of 70.6%, and 75.6%, respectively, and specificity of 73.4% and 97.1%, respectively. The combination of NS1 and IgM tests enhanced diagnosis. Thus rapid dengue NS1 antigen and IgM antibody tests are highly appropriate for diagnosis of dengue infection as it is rapid, easily applicable, sensitive and highly specific.

Authors+Show Affiliations

Department of Biochemistry, Center of Excellence in Clinical Virology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21706935

Citation

Tontulawat, Praewpilai, et al. "Evaluation of Rapid Immunochromatographic NS1 Test, Anti-dengue IgM Test, Semi-nested PCR and IgM ELISA for Detection of Dengue Virus." The Southeast Asian Journal of Tropical Medicine and Public Health, vol. 42, no. 3, 2011, pp. 570-8.
Tontulawat P, Pongsiri P, Thongmee C, et al. Evaluation of rapid immunochromatographic NS1 test, anti-dengue IgM test, semi-nested PCR and IgM ELISA for detection of dengue virus. Southeast Asian J Trop Med Public Health. 2011;42(3):570-8.
Tontulawat, P., Pongsiri, P., Thongmee, C., Theamboonlers, A., Kamolvarin, N., & Poovorawan, Y. (2011). Evaluation of rapid immunochromatographic NS1 test, anti-dengue IgM test, semi-nested PCR and IgM ELISA for detection of dengue virus. The Southeast Asian Journal of Tropical Medicine and Public Health, 42(3), 570-8.
Tontulawat P, et al. Evaluation of Rapid Immunochromatographic NS1 Test, Anti-dengue IgM Test, Semi-nested PCR and IgM ELISA for Detection of Dengue Virus. Southeast Asian J Trop Med Public Health. 2011;42(3):570-8. PubMed PMID: 21706935.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of rapid immunochromatographic NS1 test, anti-dengue IgM test, semi-nested PCR and IgM ELISA for detection of dengue virus. AU - Tontulawat,Praewpilai, AU - Pongsiri,Piyathida, AU - Thongmee,Chittima, AU - Theamboonlers,Apiradee, AU - Kamolvarin,Nuantip, AU - Poovorawan,Yong, PY - 2011/6/29/entrez PY - 2011/6/29/pubmed PY - 2011/10/5/medline SP - 570 EP - 8 JF - The Southeast Asian journal of tropical medicine and public health JO - Southeast Asian J Trop Med Public Health VL - 42 IS - 3 N2 - Dengue virus (DENV) causes various clinical symptoms of differing severity based on time of infections. The existing laboratory methods, semi-nested PCR and Dengue IgM ELISA, still have limitations for diagnosis. A commercially available rapid immunochromatographic dengue NS1 antigen and IgM antibody tests in comparison with semi-nested PCR and IgM ELISA for confirmation of DENV infection were evaluated. In total, 237 single acute serum specimens and 50 paired sera of dengue patients were examined using the rapid dengue NS1 antigen test, IgM antibody test, semi-nested PCR and Dengue IgM ELISA. The NS1 and IgM rapid tests showed sensitivity of 70.6%, and 75.6%, respectively, and specificity of 73.4% and 97.1%, respectively. The combination of NS1 and IgM tests enhanced diagnosis. Thus rapid dengue NS1 antigen and IgM antibody tests are highly appropriate for diagnosis of dengue infection as it is rapid, easily applicable, sensitive and highly specific. SN - 0125-1562 UR - https://www.unboundmedicine.com/medline/citation/21706935/Evaluation_of_rapid_immunochromatographic_NS1_test_anti_dengue_IgM_test_semi_nested_PCR_and_IgM_ELISA_for_detection_of_dengue_virus_ L2 - https://medlineplus.gov/dengue.html DB - PRIME DP - Unbound Medicine ER -