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Induction of apoptosis in human promyelocytic leukemia HL60 cells by an extract from Erythrina suberosa stem bark.
Nutr Cancer 2011; 63(5):802-13NC

Abstract

In this study, the apoptosis-inducing effect of an alcoholic extract from Erythrina suberosa stem bark (ESB) was investigated using human promyelocytic leukemia HL60 cells. Cell viability was estimated by MTT assay. We found that the ESB inhibited cell proliferation in a dose- and time-dependent manner. A series of well-documented morphological changes, such as cell shrinkage, condensation of nuclear chromatin, and nuclear fragmentation, were observed by fluorescence microscopy. The gold standard scanning electron micrographs showed apoptotic bodies and formation of blebs. Cell cycle analysis showed a significant increase in Sub G(0) population of cells above 50 μg/ml. ESB treatment resulted in a dose-dependent increase in annexin V positive cells. Increase in intracellular ROS production up to sixfold was detected in ESB-treated HL60 cells by DCFH-DA assay. Dissipation of mitochondrial membrane potential of intact cells accompanied by increase in cytosolic cytochrome c was observed, which was followed by activation of caspase-9 and -3 but not caspase-8. DNA fragmentation analysis revealed typical ladders as early as 18 h indicative of caspase-3 role in the apoptotic pathway. The overall results suggest that ESB induces mitochondria-mediated intrinsic apoptotic pathway in HL60 cells and might have therapeutic value against human leukemia.

Authors+Show Affiliations

Cancer Pharmacology Division, Indian Institute of Integrative Medicine, Jammu, India. satyamka@gmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21711175

Citation

Agrawal, Satyam Kumar, et al. "Induction of Apoptosis in Human Promyelocytic Leukemia HL60 Cells By an Extract From Erythrina Suberosa Stem Bark." Nutrition and Cancer, vol. 63, no. 5, 2011, pp. 802-13.
Agrawal SK, Agrawal M, Sharma PR, et al. Induction of apoptosis in human promyelocytic leukemia HL60 cells by an extract from Erythrina suberosa stem bark. Nutr Cancer. 2011;63(5):802-13.
Agrawal, S. K., Agrawal, M., Sharma, P. R., Gupta, B. D., Arora, S., & Saxena, A. K. (2011). Induction of apoptosis in human promyelocytic leukemia HL60 cells by an extract from Erythrina suberosa stem bark. Nutrition and Cancer, 63(5), pp. 802-13. doi:10.1080/01635581.2011.573900.
Agrawal SK, et al. Induction of Apoptosis in Human Promyelocytic Leukemia HL60 Cells By an Extract From Erythrina Suberosa Stem Bark. Nutr Cancer. 2011;63(5):802-13. PubMed PMID: 21711175.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Induction of apoptosis in human promyelocytic leukemia HL60 cells by an extract from Erythrina suberosa stem bark. AU - Agrawal,Satyam Kumar, AU - Agrawal,Madhunika, AU - Sharma,Parduman Raj, AU - Gupta,Bishan Datt, AU - Arora,Saroj, AU - Saxena,Ajit Kumar, Y1 - 2011/06/28/ PY - 2011/6/30/entrez PY - 2011/6/30/pubmed PY - 2011/12/14/medline SP - 802 EP - 13 JF - Nutrition and cancer JO - Nutr Cancer VL - 63 IS - 5 N2 - In this study, the apoptosis-inducing effect of an alcoholic extract from Erythrina suberosa stem bark (ESB) was investigated using human promyelocytic leukemia HL60 cells. Cell viability was estimated by MTT assay. We found that the ESB inhibited cell proliferation in a dose- and time-dependent manner. A series of well-documented morphological changes, such as cell shrinkage, condensation of nuclear chromatin, and nuclear fragmentation, were observed by fluorescence microscopy. The gold standard scanning electron micrographs showed apoptotic bodies and formation of blebs. Cell cycle analysis showed a significant increase in Sub G(0) population of cells above 50 μg/ml. ESB treatment resulted in a dose-dependent increase in annexin V positive cells. Increase in intracellular ROS production up to sixfold was detected in ESB-treated HL60 cells by DCFH-DA assay. Dissipation of mitochondrial membrane potential of intact cells accompanied by increase in cytosolic cytochrome c was observed, which was followed by activation of caspase-9 and -3 but not caspase-8. DNA fragmentation analysis revealed typical ladders as early as 18 h indicative of caspase-3 role in the apoptotic pathway. The overall results suggest that ESB induces mitochondria-mediated intrinsic apoptotic pathway in HL60 cells and might have therapeutic value against human leukemia. SN - 1532-7914 UR - https://www.unboundmedicine.com/medline/citation/21711175/Induction_of_apoptosis_in_human_promyelocytic_leukemia_HL60_cells_by_an_extract_from_Erythrina_suberosa_stem_bark_ L2 - http://www.tandfonline.com/doi/full/10.1080/01635581.2011.573900 DB - PRIME DP - Unbound Medicine ER -