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Human natural killer cells expressing the memory-associated marker CD45RO from tuberculous pleurisy respond more strongly and rapidly than CD45RO- natural killer cells following stimulation with interleukin-12.

Abstract

Natural killer (NK) cells are known as innate immune lymphocytes that respond rapidly when challenged by pathogens but little is known about adaptive immune features including memory related to NK cells from human beings. In the present study, we demonstrate for the first time that human NK cells expressing the memory-associated marker CD45RO were persistent in pleural fluid cells (PFCs) from tuberculous patients. CD45RO(+) NK cells produced significantly more interferon-γ and were more cytotoxic compared with CD45RO(-) NK cells from PFCs when stimulated with interleukin-12 (IL-12). Consistently, IL-12 enhanced the expression of granzyme B, CD69, CD25, NKG2D, IL-12 receptors β1 and β2 on CD45RO(+) NK cells from PFCs. Our experiments contribute to a better understanding of the NK cells from PFCs and indicate that human CD45RO(+) NK cells from PFCs expressing a 'memory-like' phenotype may have an important role in defending against infection by Mycobacterium tuberculosis.

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  • Authors+Show Affiliations

    ,

    Institute of Immunology, Zhongshan School of Medicine, Key Laboratory of Tropical Disease Control Research of Ministry of Education, Sun Yat-sen University, Guangzhou Chest Hospital of Guangzhou, Guangzhou, China.

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    Source

    Immunology 134:1 2011 Sep pg 41-9

    MeSH

    Adolescent
    Adult
    Aged
    Antigens, CD
    Antigens, Differentiation, T-Lymphocyte
    CD56 Antigen
    Cell Count
    Cytotoxicity, Immunologic
    Female
    GPI-Linked Proteins
    Granzymes
    Humans
    Interferon-gamma
    Interleukin-12
    Interleukin-2 Receptor alpha Subunit
    K562 Cells
    Killer Cells, Natural
    Lectins, C-Type
    Leukocyte Common Antigens
    Leukocytes, Mononuclear
    Lymphocyte Activation
    Lymphocyte Subsets
    Male
    Middle Aged
    NK Cell Lectin-Like Receptor Subfamily C
    NK Cell Lectin-Like Receptor Subfamily K
    Pleural Effusion
    Receptors, IgG
    Receptors, Interleukin-12
    Tuberculosis, Pleural
    Young Adult

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    21711347

    Citation

    Fu, Xiaoying, et al. "Human Natural Killer Cells Expressing the Memory-associated Marker CD45RO From Tuberculous Pleurisy Respond More Strongly and Rapidly Than CD45RO- Natural Killer Cells Following Stimulation With Interleukin-12." Immunology, vol. 134, no. 1, 2011, pp. 41-9.
    Fu X, Liu Y, Li L, et al. Human natural killer cells expressing the memory-associated marker CD45RO from tuberculous pleurisy respond more strongly and rapidly than CD45RO- natural killer cells following stimulation with interleukin-12. Immunology. 2011;134(1):41-9.
    Fu, X., Liu, Y., Li, L., Li, Q., Qiao, D., Wang, H., ... Wu, C. (2011). Human natural killer cells expressing the memory-associated marker CD45RO from tuberculous pleurisy respond more strongly and rapidly than CD45RO- natural killer cells following stimulation with interleukin-12. Immunology, 134(1), pp. 41-9. doi:10.1111/j.1365-2567.2011.03464.x.
    Fu X, et al. Human Natural Killer Cells Expressing the Memory-associated Marker CD45RO From Tuberculous Pleurisy Respond More Strongly and Rapidly Than CD45RO- Natural Killer Cells Following Stimulation With Interleukin-12. Immunology. 2011;134(1):41-9. PubMed PMID: 21711347.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Human natural killer cells expressing the memory-associated marker CD45RO from tuberculous pleurisy respond more strongly and rapidly than CD45RO- natural killer cells following stimulation with interleukin-12. AU - Fu,Xiaoying, AU - Liu,Yun, AU - Li,Li, AU - Li,Qin, AU - Qiao,Dan, AU - Wang,Hui, AU - Lao,Suihua, AU - Fan,Yanying, AU - Wu,Changyou, Y1 - 2011/06/29/ PY - 2011/6/30/entrez PY - 2011/6/30/pubmed PY - 2011/10/14/medline SP - 41 EP - 9 JF - Immunology JO - Immunology VL - 134 IS - 1 N2 - Natural killer (NK) cells are known as innate immune lymphocytes that respond rapidly when challenged by pathogens but little is known about adaptive immune features including memory related to NK cells from human beings. In the present study, we demonstrate for the first time that human NK cells expressing the memory-associated marker CD45RO were persistent in pleural fluid cells (PFCs) from tuberculous patients. CD45RO(+) NK cells produced significantly more interferon-γ and were more cytotoxic compared with CD45RO(-) NK cells from PFCs when stimulated with interleukin-12 (IL-12). Consistently, IL-12 enhanced the expression of granzyme B, CD69, CD25, NKG2D, IL-12 receptors β1 and β2 on CD45RO(+) NK cells from PFCs. Our experiments contribute to a better understanding of the NK cells from PFCs and indicate that human CD45RO(+) NK cells from PFCs expressing a 'memory-like' phenotype may have an important role in defending against infection by Mycobacterium tuberculosis. SN - 1365-2567 UR - https://www.unboundmedicine.com/medline/citation/21711347/Human_natural_killer_cells_expressing_the_memory_associated_marker_CD45RO_from_tuberculous_pleurisy_respond_more_strongly_and_rapidly_than_CD45RO__natural_killer_cells_following_stimulation_with_interleukin_12_ L2 - https://doi.org/10.1111/j.1365-2567.2011.03464.x DB - PRIME DP - Unbound Medicine ER -