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Rapid isolation of some antiepileptic hydantoins and their analogues with Sep-Pak C18 cartridges and capillary gas chromatography with splitless injection.
Z Rechtsmed. 1990; 103(7):479-85.ZR

Abstract

A simple and rapid method for isolation of seven antiepileptics (2 hydantoin, 2 oxazolidin, and 3 suximide derivatives) from urine and plasma is presented. Urine and plasma (1 ml) samples containing seven antiepileptics were mixed with distilled water (4 ml), and the sample solution was poured into a pretreated Sep-Pak C18 cartridge; this was washed with water and chloroform/methanol was passed through it to elute the antiepileptics. The eluate was mixed with isoamyl acetate and evaporated under a stream of N2. The drugs were detected by gas chromatography with fused silica capillary columns, splitless injection and flame ionization detection. Separation of the seven antiepileptics from each other and from impurities was satisfactory with the use of an SPB-1 capillary column. The detection limit for the seven antiepileptics with the present method was 0.1-1.0 microgram/ml urine or plasma. The recovery of the drugs from urine and plasma was more than 70% and 50%, respectively.

Authors+Show Affiliations

Department of Legal Medicine, Hamamatsu University School of Medicine, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

2171244

Citation

Kumazawa, T, et al. "Rapid Isolation of some Antiepileptic Hydantoins and Their Analogues With Sep-Pak C18 Cartridges and Capillary Gas Chromatography With Splitless Injection." Zeitschrift Fur Rechtsmedizin. Journal of Legal Medicine, vol. 103, no. 7, 1990, pp. 479-85.
Kumazawa T, Suzuki O, Seno H, et al. Rapid isolation of some antiepileptic hydantoins and their analogues with Sep-Pak C18 cartridges and capillary gas chromatography with splitless injection. Z Rechtsmed. 1990;103(7):479-85.
Kumazawa, T., Suzuki, O., Seno, H., Ishikawa, Y., & Hattori, H. (1990). Rapid isolation of some antiepileptic hydantoins and their analogues with Sep-Pak C18 cartridges and capillary gas chromatography with splitless injection. Zeitschrift Fur Rechtsmedizin. Journal of Legal Medicine, 103(7), 479-85.
Kumazawa T, et al. Rapid Isolation of some Antiepileptic Hydantoins and Their Analogues With Sep-Pak C18 Cartridges and Capillary Gas Chromatography With Splitless Injection. Z Rechtsmed. 1990;103(7):479-85. PubMed PMID: 2171244.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rapid isolation of some antiepileptic hydantoins and their analogues with Sep-Pak C18 cartridges and capillary gas chromatography with splitless injection. AU - Kumazawa,T, AU - Suzuki,O, AU - Seno,H, AU - Ishikawa,Y, AU - Hattori,H, PY - 1990/1/1/pubmed PY - 1990/1/1/medline PY - 1990/1/1/entrez SP - 479 EP - 85 JF - Zeitschrift fur Rechtsmedizin. Journal of legal medicine JO - Z Rechtsmed VL - 103 IS - 7 N2 - A simple and rapid method for isolation of seven antiepileptics (2 hydantoin, 2 oxazolidin, and 3 suximide derivatives) from urine and plasma is presented. Urine and plasma (1 ml) samples containing seven antiepileptics were mixed with distilled water (4 ml), and the sample solution was poured into a pretreated Sep-Pak C18 cartridge; this was washed with water and chloroform/methanol was passed through it to elute the antiepileptics. The eluate was mixed with isoamyl acetate and evaporated under a stream of N2. The drugs were detected by gas chromatography with fused silica capillary columns, splitless injection and flame ionization detection. Separation of the seven antiepileptics from each other and from impurities was satisfactory with the use of an SPB-1 capillary column. The detection limit for the seven antiepileptics with the present method was 0.1-1.0 microgram/ml urine or plasma. The recovery of the drugs from urine and plasma was more than 70% and 50%, respectively. SN - 0044-3433 UR - https://www.unboundmedicine.com/medline/citation/2171244/Rapid_isolation_of_some_antiepileptic_hydantoins_and_their_analogues_with_Sep_Pak_C18_cartridges_and_capillary_gas_chromatography_with_splitless_injection_ DB - PRIME DP - Unbound Medicine ER -