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Effects of apomorphine on locomotive activity and monoamine metabolism: a dose related study.
Pak J Pharm Sci 2011; 24(3):315-21PJ

Abstract

We have monitored dose dependent effects of apomorphine on motor activity and monoamine metabolism. Behavioral sensitization and craving, which develop upon repeated treatment with dopamine receptor agonist apomorphine, are major limitations of the therapeutic use of apomorphine in Parkinson's patients. Effects of single (intraperitoneal) injection of apomorphine at different doses (i.e., 1.0, 2.0 & 4.0 mg/kg) on exploration in a novel environment (open field) and locomotion in a familiar environment (home cage) were investigated. Results show significantly enhanced activity in home cage (monitored 5min post injection) in a dose dependent manner. However, no significant influence of apomorphine on exploration of open field was observed in the present study (monitored 15 min and 40 min post injection). Animals were decapitated 1 hr post apomorphine injection and whole brains of animals were collected and stored at -70°C. Biogenic amines (i.e., 5-Hydroxytryptamine and dopamine) and metabolites (i.e., Dihydroxyphenylacetic acid, Homovanillic acid & 5-Hydroxyindoleacetic acid) were estimated by reverse phase High Performance Liquid Chromatography with electrochemical detector (HPLC-EC). Effect of low (1.0mg/kg) dose of apomorphine was found to be non-significant on 5-Hydroxytryptamine (5-HT), 5-Hydroxyindoleacetic acid (5-HIAA) and dopamine (DA) levels. Moderate (2.0 mg/kg) dose of drug increased (p<0.05) levels of Homovanillic acid (HVA). Whereas, high (4.0 mg/kg) dose of apomorphine decreased Dihydroxyphenylacetic acid (DOPAC) levels. Results could be helpful in elucidating the effect of apomorphine at different doses and its implication for extending therapeutics in Parkinson's and related disorders.

Authors+Show Affiliations

Neurochemistry and Biochemical Neuropharmacology Research Laboratory, Department of Biochemistry, University of Karachi, Karachi-75270, Pakistan. huma_biochemist@yahoo.comNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

21715264

Citation

Ikram, Huma, et al. "Effects of Apomorphine On Locomotive Activity and Monoamine Metabolism: a Dose Related Study." Pakistan Journal of Pharmaceutical Sciences, vol. 24, no. 3, 2011, pp. 315-21.
Ikram H, Ahmad S, Haleem DJ. Effects of apomorphine on locomotive activity and monoamine metabolism: a dose related study. Pak J Pharm Sci. 2011;24(3):315-21.
Ikram, H., Ahmad, S., & Haleem, D. J. (2011). Effects of apomorphine on locomotive activity and monoamine metabolism: a dose related study. Pakistan Journal of Pharmaceutical Sciences, 24(3), pp. 315-21.
Ikram H, Ahmad S, Haleem DJ. Effects of Apomorphine On Locomotive Activity and Monoamine Metabolism: a Dose Related Study. Pak J Pharm Sci. 2011;24(3):315-21. PubMed PMID: 21715264.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of apomorphine on locomotive activity and monoamine metabolism: a dose related study. AU - Ikram,Huma, AU - Ahmad,Shoaib, AU - Haleem,Darakhshan Jabeen, PY - 2011/7/1/entrez PY - 2011/7/1/pubmed PY - 2011/9/16/medline SP - 315 EP - 21 JF - Pakistan journal of pharmaceutical sciences JO - Pak J Pharm Sci VL - 24 IS - 3 N2 - We have monitored dose dependent effects of apomorphine on motor activity and monoamine metabolism. Behavioral sensitization and craving, which develop upon repeated treatment with dopamine receptor agonist apomorphine, are major limitations of the therapeutic use of apomorphine in Parkinson's patients. Effects of single (intraperitoneal) injection of apomorphine at different doses (i.e., 1.0, 2.0 & 4.0 mg/kg) on exploration in a novel environment (open field) and locomotion in a familiar environment (home cage) were investigated. Results show significantly enhanced activity in home cage (monitored 5min post injection) in a dose dependent manner. However, no significant influence of apomorphine on exploration of open field was observed in the present study (monitored 15 min and 40 min post injection). Animals were decapitated 1 hr post apomorphine injection and whole brains of animals were collected and stored at -70°C. Biogenic amines (i.e., 5-Hydroxytryptamine and dopamine) and metabolites (i.e., Dihydroxyphenylacetic acid, Homovanillic acid & 5-Hydroxyindoleacetic acid) were estimated by reverse phase High Performance Liquid Chromatography with electrochemical detector (HPLC-EC). Effect of low (1.0mg/kg) dose of apomorphine was found to be non-significant on 5-Hydroxytryptamine (5-HT), 5-Hydroxyindoleacetic acid (5-HIAA) and dopamine (DA) levels. Moderate (2.0 mg/kg) dose of drug increased (p<0.05) levels of Homovanillic acid (HVA). Whereas, high (4.0 mg/kg) dose of apomorphine decreased Dihydroxyphenylacetic acid (DOPAC) levels. Results could be helpful in elucidating the effect of apomorphine at different doses and its implication for extending therapeutics in Parkinson's and related disorders. SN - 1011-601X UR - https://www.unboundmedicine.com/medline/citation/21715264/Effects_of_apomorphine_on_locomotive_activity_and_monoamine_metabolism:_a_dose_related_study_ DB - PRIME DP - Unbound Medicine ER -