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Esenbeckia leiocarpa Engl. inhibits inflammation in a carrageenan-induced murine model of pleurisy.
J Pharm Pharmacol. 2011 Aug; 63(8):1091-102.JP

Abstract

OBJECTIVES

The aim of this study was to investigate the anti-inflammatory effects of the crude hydroalcoholic extract (CHE) isolated from Esenbeckia leiocarpa Engl., and fractions and subfractions derived from it.

METHODS

Dried E. leiocarpa Engl. bark was macerated and extracted with ethanol to obtain the CHE. The n-hexane, ethyl acetate, aqueous and alkaloid fractions, as well as two alkaloid subfractions (polar and nonpolar) were obtained from the CHE. A preliminary analysis using thin-layer chromatography was performed. Capillary electrophoresis, physical characteristics and spectral data produced by IR analysis and nuclear magnetic resonance (¹H and ¹³C NMR), and mass spectrometry analysis were used to identify and elucidate the structure of the major compounds. Swiss mice were used in a carrageenan-induced pleurisy model. Pro-inflammatory parameters (leukocyte and exudate concentrations, myeloperoxidase and adenosine-deaminase activity, and nitrate/nitrite, interleukin 1β and tumour necrosis factor α levels) were quantified in exudates at 4 h after carrageenan-induced pleurisy in mice.

KEY FINDINGS

The dihydrocorynantheol alkaloid was isolated as the majority compound in the CHE, ethyl acetate and alkaloid fractions, and in the polar and nonpolar alkaloid subfractions. The CHE, fractions and subfractions inhibited the increases in leukocyte and exudate concentrations, myeloperoxidase and adenosine-deaminase activity, and nitrite/nitrate, interleukin 1β, and tumour necrosis factor α levels (P<0.05) in the fluid secreted from the pleural cavity of the carrageenan-treated mice.

CONCLUSIONS

E. leiocarpa Engl. showed significant in vivo anti-inflammatory action by inhibiting the inflammation caused by carrageenan. This effect may be, in part, due to the dihydrocorynantheol alkaloid, which was identified as the majority compound isolated from E. leiocarpa bark.

Authors+Show Affiliations

Department of Clinical Analysis, Centre for Health Sciences, Federal University of Santa Catarina, Campus Universitário, Trindade, Florianópolis, SC, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

21718293

Citation

Pozzatti, Patrícia, et al. "Esenbeckia Leiocarpa Engl. Inhibits Inflammation in a Carrageenan-induced Murine Model of Pleurisy." The Journal of Pharmacy and Pharmacology, vol. 63, no. 8, 2011, pp. 1091-102.
Pozzatti P, dos Reis GO, Pereira DF, et al. Esenbeckia leiocarpa Engl. inhibits inflammation in a carrageenan-induced murine model of pleurisy. J Pharm Pharmacol. 2011;63(8):1091-102.
Pozzatti, P., dos Reis, G. O., Pereira, D. F., Heller, M., Micke, G. A., Horst, H., Pizzolatti, M. G., & Fröde, T. S. (2011). Esenbeckia leiocarpa Engl. inhibits inflammation in a carrageenan-induced murine model of pleurisy. The Journal of Pharmacy and Pharmacology, 63(8), 1091-102. https://doi.org/10.1111/j.2042-7158.2011.01311.x
Pozzatti P, et al. Esenbeckia Leiocarpa Engl. Inhibits Inflammation in a Carrageenan-induced Murine Model of Pleurisy. J Pharm Pharmacol. 2011;63(8):1091-102. PubMed PMID: 21718293.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Esenbeckia leiocarpa Engl. inhibits inflammation in a carrageenan-induced murine model of pleurisy. AU - Pozzatti,Patrícia, AU - dos Reis,Gustavo Oliveira, AU - Pereira,Danielle Fontana, AU - Heller,Melina, AU - Micke,Gustavo Amadeu, AU - Horst,Heros, AU - Pizzolatti,Moacir Geraldo, AU - Fröde,Tânia Silvia, Y1 - 2011/06/15/ PY - 2011/7/2/entrez PY - 2011/7/2/pubmed PY - 2012/1/10/medline SP - 1091 EP - 102 JF - The Journal of pharmacy and pharmacology JO - J. Pharm. Pharmacol. VL - 63 IS - 8 N2 - OBJECTIVES: The aim of this study was to investigate the anti-inflammatory effects of the crude hydroalcoholic extract (CHE) isolated from Esenbeckia leiocarpa Engl., and fractions and subfractions derived from it. METHODS: Dried E. leiocarpa Engl. bark was macerated and extracted with ethanol to obtain the CHE. The n-hexane, ethyl acetate, aqueous and alkaloid fractions, as well as two alkaloid subfractions (polar and nonpolar) were obtained from the CHE. A preliminary analysis using thin-layer chromatography was performed. Capillary electrophoresis, physical characteristics and spectral data produced by IR analysis and nuclear magnetic resonance (¹H and ¹³C NMR), and mass spectrometry analysis were used to identify and elucidate the structure of the major compounds. Swiss mice were used in a carrageenan-induced pleurisy model. Pro-inflammatory parameters (leukocyte and exudate concentrations, myeloperoxidase and adenosine-deaminase activity, and nitrate/nitrite, interleukin 1β and tumour necrosis factor α levels) were quantified in exudates at 4 h after carrageenan-induced pleurisy in mice. KEY FINDINGS: The dihydrocorynantheol alkaloid was isolated as the majority compound in the CHE, ethyl acetate and alkaloid fractions, and in the polar and nonpolar alkaloid subfractions. The CHE, fractions and subfractions inhibited the increases in leukocyte and exudate concentrations, myeloperoxidase and adenosine-deaminase activity, and nitrite/nitrate, interleukin 1β, and tumour necrosis factor α levels (P<0.05) in the fluid secreted from the pleural cavity of the carrageenan-treated mice. CONCLUSIONS: E. leiocarpa Engl. showed significant in vivo anti-inflammatory action by inhibiting the inflammation caused by carrageenan. This effect may be, in part, due to the dihydrocorynantheol alkaloid, which was identified as the majority compound isolated from E. leiocarpa bark. SN - 2042-7158 UR - https://www.unboundmedicine.com/medline/citation/21718293/Esenbeckia_leiocarpa_Engl__inhibits_inflammation_in_a_carrageenan_induced_murine_model_of_pleurisy_ L2 - https://doi.org/10.1111/j.2042-7158.2011.01311.x DB - PRIME DP - Unbound Medicine ER -