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Enhanced production of plasmid DNA by engineered Escherichia coli strains.
J Biotechnol. 2012 Apr 30; 158(4):211-4.JB

Abstract

Escherichia coli strains VH33 (PTS⁻ GalP⁺ strain displaying a strongly reduced overflow metabolism) and VH34 (additionally lacking the pyruvate kinase A) were evaluated for the production of a plasmid DNA (pDNA) vaccine. The parent (W3110) and mutant strains were cultured using 10 g of glucose/L. While the specific growth rates of the three strains were similar, they presented differences in the accumulation of acetate. W3110 accumulated up to 4 g/L of acetate, VH33 produced 1.4 g/L, and VH34 only 0.78 g/L. VH33 and VH34 produced 76% and 300% more pDNA than W3110. Moreover, VH34 demanded 33% less oxygen than VH33 and W3110, which can be advantageous for large-scale applications.

Authors+Show Affiliations

Departamento de Procesos y Tecnología, Universidad Autónoma Metropolitana-Cuajimalpa, Artificios No. 40, Col. Miguel Hidalgo, Del. Álvaro Obregón, México DF, CP 01120, Mexico.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21718724

Citation

Pablos, Tania E., et al. "Enhanced Production of Plasmid DNA By Engineered Escherichia Coli Strains." Journal of Biotechnology, vol. 158, no. 4, 2012, pp. 211-4.
Pablos TE, Soto R, Mora EM, et al. Enhanced production of plasmid DNA by engineered Escherichia coli strains. J Biotechnol. 2012;158(4):211-4.
Pablos, T. E., Soto, R., Mora, E. M., Le Borgne, S., Ramírez, O. T., Gosset, G., & Lara, A. R. (2012). Enhanced production of plasmid DNA by engineered Escherichia coli strains. Journal of Biotechnology, 158(4), 211-4. https://doi.org/10.1016/j.jbiotec.2011.04.015
Pablos TE, et al. Enhanced Production of Plasmid DNA By Engineered Escherichia Coli Strains. J Biotechnol. 2012 Apr 30;158(4):211-4. PubMed PMID: 21718724.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Enhanced production of plasmid DNA by engineered Escherichia coli strains. AU - Pablos,Tania E, AU - Soto,René, AU - Mora,Eugenio Meza, AU - Le Borgne,Sylvie, AU - Ramírez,Octavio T, AU - Gosset,Guillermo, AU - Lara,Alvaro R, Y1 - 2011/06/17/ PY - 2011/01/13/received PY - 2011/04/20/revised PY - 2011/04/29/accepted PY - 2011/7/2/entrez PY - 2011/7/2/pubmed PY - 2012/10/17/medline SP - 211 EP - 4 JF - Journal of biotechnology JO - J. Biotechnol. VL - 158 IS - 4 N2 - Escherichia coli strains VH33 (PTS⁻ GalP⁺ strain displaying a strongly reduced overflow metabolism) and VH34 (additionally lacking the pyruvate kinase A) were evaluated for the production of a plasmid DNA (pDNA) vaccine. The parent (W3110) and mutant strains were cultured using 10 g of glucose/L. While the specific growth rates of the three strains were similar, they presented differences in the accumulation of acetate. W3110 accumulated up to 4 g/L of acetate, VH33 produced 1.4 g/L, and VH34 only 0.78 g/L. VH33 and VH34 produced 76% and 300% more pDNA than W3110. Moreover, VH34 demanded 33% less oxygen than VH33 and W3110, which can be advantageous for large-scale applications. SN - 1873-4863 UR - https://www.unboundmedicine.com/medline/citation/21718724/Enhanced_production_of_plasmid_DNA_by_engineered_Escherichia_coli_strains_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0168-1656(11)00231-8 DB - PRIME DP - Unbound Medicine ER -