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IκBα glutathionylation and reduced histone H3 phosphorylation inhibit eotaxin and RANTES.
Eur Respir J. 2011 Dec; 38(6):1444-52.ER

Abstract

Airway smooth muscle cells (ASMCs) secrete eotaxin and RANTES (regulated on activation, normal T-cell expressed and secreted) in response to tumour necrosis factor (TNF)-α, which is inhibited by the nuclear factor (NF)-κB inhibitor dimethylfumarate (DMF). NF-κB/IκB (inhibitor of NF-κB) glutathionylation and changes in chromatin remodelling can inhibit NF-κB activity. In this study, we determined whether NF-κB/IκB glutathionylation and reduced histone H3 phosphorylation might underlie the inhibitory effect of DMF on NF-κB activity, and eotaxin and RANTES secretion. Primary human ASMCs were treated with DMF, diamide and/or glutathione (GSH) ethylester (OEt) prior to TNF-α stimulation and were subsequently analysed by ELISA, electrophoretic mobility shift assay, immunofluorescence, co-immunoprecipitation or immunoblotting. DMF reduced intracellular GSH and induced IκBα glutathionylation (IκBα-SSG), which inhibited IκBα degradation, NF-κB p65 nuclear entry and NF-κB/DNA binding. In addition, DMF inhibited the phosphorylation of histone H3, which was possibly mediated by the inhibitory effect of DMF on mitogen- and stress-activated protein kinase (MSK)-1. However, p38 mitogen-activated protein kinase (MAPK), extracellular signal-regulated kinase MAPK and MAPK phosphatase-1, upstream of MSK-1, were not inhibited by DMF. Importantly, DMF-mediated effects on NF-κB, histone H3, eotaxin and RANTES were reversed by addition of GSH-OEt. Our data suggest that DMF inhibits NF-κB-dependent eotaxin and RANTES secretion by reduction of GSH with subsequent induction of IκBα-SSG and inhibition of histone H3 phosphorylation. Our findings offer new potential drug targets to reduce airway inflammation in asthma.

Authors+Show Affiliations

Respiratory Research Group, Faculty of Pharmacy, University of Sydney, Sydney, Australia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21719482

Citation

Seidel, P, et al. "IκBα Glutathionylation and Reduced Histone H3 Phosphorylation Inhibit Eotaxin and RANTES." The European Respiratory Journal, vol. 38, no. 6, 2011, pp. 1444-52.
Seidel P, Roth M, Ge Q, et al. IκBα glutathionylation and reduced histone H3 phosphorylation inhibit eotaxin and RANTES. Eur Respir J. 2011;38(6):1444-52.
Seidel, P., Roth, M., Ge, Q., Merfort, I., S'ng, C. T., & Ammit, A. J. (2011). IκBα glutathionylation and reduced histone H3 phosphorylation inhibit eotaxin and RANTES. The European Respiratory Journal, 38(6), 1444-52. https://doi.org/10.1183/09031936.00129610
Seidel P, et al. IκBα Glutathionylation and Reduced Histone H3 Phosphorylation Inhibit Eotaxin and RANTES. Eur Respir J. 2011;38(6):1444-52. PubMed PMID: 21719482.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - IκBα glutathionylation and reduced histone H3 phosphorylation inhibit eotaxin and RANTES. AU - Seidel,P, AU - Roth,M, AU - Ge,Q, AU - Merfort,I, AU - S'ng,C T, AU - Ammit,A J, Y1 - 2011/06/30/ PY - 2011/7/2/entrez PY - 2011/7/2/pubmed PY - 2012/4/17/medline SP - 1444 EP - 52 JF - The European respiratory journal JO - Eur Respir J VL - 38 IS - 6 N2 - Airway smooth muscle cells (ASMCs) secrete eotaxin and RANTES (regulated on activation, normal T-cell expressed and secreted) in response to tumour necrosis factor (TNF)-α, which is inhibited by the nuclear factor (NF)-κB inhibitor dimethylfumarate (DMF). NF-κB/IκB (inhibitor of NF-κB) glutathionylation and changes in chromatin remodelling can inhibit NF-κB activity. In this study, we determined whether NF-κB/IκB glutathionylation and reduced histone H3 phosphorylation might underlie the inhibitory effect of DMF on NF-κB activity, and eotaxin and RANTES secretion. Primary human ASMCs were treated with DMF, diamide and/or glutathione (GSH) ethylester (OEt) prior to TNF-α stimulation and were subsequently analysed by ELISA, electrophoretic mobility shift assay, immunofluorescence, co-immunoprecipitation or immunoblotting. DMF reduced intracellular GSH and induced IκBα glutathionylation (IκBα-SSG), which inhibited IκBα degradation, NF-κB p65 nuclear entry and NF-κB/DNA binding. In addition, DMF inhibited the phosphorylation of histone H3, which was possibly mediated by the inhibitory effect of DMF on mitogen- and stress-activated protein kinase (MSK)-1. However, p38 mitogen-activated protein kinase (MAPK), extracellular signal-regulated kinase MAPK and MAPK phosphatase-1, upstream of MSK-1, were not inhibited by DMF. Importantly, DMF-mediated effects on NF-κB, histone H3, eotaxin and RANTES were reversed by addition of GSH-OEt. Our data suggest that DMF inhibits NF-κB-dependent eotaxin and RANTES secretion by reduction of GSH with subsequent induction of IκBα-SSG and inhibition of histone H3 phosphorylation. Our findings offer new potential drug targets to reduce airway inflammation in asthma. SN - 1399-3003 UR - https://www.unboundmedicine.com/medline/citation/21719482/IκBα_glutathionylation_and_reduced_histone_H3_phosphorylation_inhibit_eotaxin_and_RANTES_ L2 - http://erj.ersjournals.com/cgi/pmidlookup?view=long&pmid=21719482 DB - PRIME DP - Unbound Medicine ER -