Tags

Type your tag names separated by a space and hit enter

Reduced nicotinamide adenine dinucleotide fluorescence lifetime detected poly(adenosine-5'-diphosphate-ribose) polymerase-1-mediated cell death and therapeutic effect of pyruvate.
J Biomed Opt. 2011 Jun; 16(6):068001.JB

Abstract

Noninvasive detection of cell death has the potential for definitive diagnosis and monitoring treatment outcomes in real time. Reduced nicotinamide adenine dinucleotide (NADH) fluorescence intensity has long been used as a noninvasive optical probe of metabolic states. NADH fluorescence lifetime has recently been studied for its potential as an alternative optical probe of cellular metabolic states and cell death. In this study, we investigated the potential using NADH fluorescence intensity and/or lifetime to detect poly(adenosine-5'-diphosphate-ribose) polymerase-1 (PARP-1)-mediated cell death in HeLa cells. We also examined if NADH signals respond to treatment by pyruvate. The mechanism of PARP-1-mediated cell death has been well studied that extensive PARP-1 activation leads to cytosolic nicotinamide adenine dinucleotide depletion resulting in glycolytic inhibition, mitochondrial failure, and death. Pyruvate could restore electron transport chain to prevent energy failure and death. Our results show that NADH fluorescence lifetime, not intensity, responded to PARP-1-mediated cell death and the rescue effect of pyruvate. This lifetime change of NADH fluorescence happened before the collapse of mitochondrial membrane potential and mitochondrial uncoupling. Together with our previous findings in staurosporine-induced cell death, we suggest that NADH fluorescence lifetime increase during cell death is mainly due to increased protein-protein interactions but not the intracellular NADH content.

Authors+Show Affiliations

National Yang-Ming University, Institute of Biophotonics, Taipei 112, Taiwan. hwwang2@ym.edu.twNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21721834

Citation

Guo, Han-Wen, et al. "Reduced Nicotinamide Adenine Dinucleotide Fluorescence Lifetime Detected Poly(adenosine-5'-diphosphate-ribose) Polymerase-1-mediated Cell Death and Therapeutic Effect of Pyruvate." Journal of Biomedical Optics, vol. 16, no. 6, 2011, p. 068001.
Guo HW, Wei YH, Wang HW. Reduced nicotinamide adenine dinucleotide fluorescence lifetime detected poly(adenosine-5'-diphosphate-ribose) polymerase-1-mediated cell death and therapeutic effect of pyruvate. J Biomed Opt. 2011;16(6):068001.
Guo, H. W., Wei, Y. H., & Wang, H. W. (2011). Reduced nicotinamide adenine dinucleotide fluorescence lifetime detected poly(adenosine-5'-diphosphate-ribose) polymerase-1-mediated cell death and therapeutic effect of pyruvate. Journal of Biomedical Optics, 16(6), 068001. https://doi.org/10.1117/1.3590204
Guo HW, Wei YH, Wang HW. Reduced Nicotinamide Adenine Dinucleotide Fluorescence Lifetime Detected Poly(adenosine-5'-diphosphate-ribose) Polymerase-1-mediated Cell Death and Therapeutic Effect of Pyruvate. J Biomed Opt. 2011;16(6):068001. PubMed PMID: 21721834.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Reduced nicotinamide adenine dinucleotide fluorescence lifetime detected poly(adenosine-5'-diphosphate-ribose) polymerase-1-mediated cell death and therapeutic effect of pyruvate. AU - Guo,Han-Wen, AU - Wei,Yau-Huei, AU - Wang,Hsing-Wen, PY - 2011/7/5/entrez PY - 2011/7/5/pubmed PY - 2011/11/9/medline SP - 068001 EP - 068001 JF - Journal of biomedical optics JO - J Biomed Opt VL - 16 IS - 6 N2 - Noninvasive detection of cell death has the potential for definitive diagnosis and monitoring treatment outcomes in real time. Reduced nicotinamide adenine dinucleotide (NADH) fluorescence intensity has long been used as a noninvasive optical probe of metabolic states. NADH fluorescence lifetime has recently been studied for its potential as an alternative optical probe of cellular metabolic states and cell death. In this study, we investigated the potential using NADH fluorescence intensity and/or lifetime to detect poly(adenosine-5'-diphosphate-ribose) polymerase-1 (PARP-1)-mediated cell death in HeLa cells. We also examined if NADH signals respond to treatment by pyruvate. The mechanism of PARP-1-mediated cell death has been well studied that extensive PARP-1 activation leads to cytosolic nicotinamide adenine dinucleotide depletion resulting in glycolytic inhibition, mitochondrial failure, and death. Pyruvate could restore electron transport chain to prevent energy failure and death. Our results show that NADH fluorescence lifetime, not intensity, responded to PARP-1-mediated cell death and the rescue effect of pyruvate. This lifetime change of NADH fluorescence happened before the collapse of mitochondrial membrane potential and mitochondrial uncoupling. Together with our previous findings in staurosporine-induced cell death, we suggest that NADH fluorescence lifetime increase during cell death is mainly due to increased protein-protein interactions but not the intracellular NADH content. SN - 1560-2281 UR - https://www.unboundmedicine.com/medline/citation/21721834/Reduced_nicotinamide_adenine_dinucleotide_fluorescence_lifetime_detected_poly_adenosine_5'_diphosphate_ribose__polymerase_1_mediated_cell_death_and_therapeutic_effect_of_pyruvate_ L2 - https://doi.org/10.1117/1.3590204 DB - PRIME DP - Unbound Medicine ER -