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Gastroprotective activity of Zanthoxylum rhoifolium Lam. in animal models.
J Ethnopharmacol. 2011 Sep 01; 137(1):700-8.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

The stem barks of Zanthoxylum rhoifolium Lam. (Rutaceae), locally known as "mamica de cadela", are popularly used in dyspepsies, stomachic, tonic, antitumoral, antipyretic and are used in treating flatulence and colic. The objective of this study was to evaluate the gastroprotective effect of the ethanolic extract of Zanthoxylum rhoifolium (EEZR) stem barks in acute gastric lesion models, investigating their possible mechanisms.

MATERIALS AND METHODS

Mice were used for the evaluation of the acute toxicity, and mice and rats to study the gastroprotective activity. The gastroprotective action of EEZR was analyzed in the absolute ethanol, HCl/ethanol and indomethacin-induced gastric lesion models in mice, hypothermic-restraint stress, and ischemia/reperfusion in rats. In the investigation of the gastroprotective mechanisms of EEZR, the participation of the NO-synthase pathway, ATP-sensitive potassium channels (K(ATP)), the levels of the non-protein sulfhydril groups (NP-SH) and the catalase activity using the ethanol-induced gastric mucosa lesion model and the quantification of the gastric mucus and the antisecretory activity through pylorus ligature model in rats were analyzed.

RESULTS

The animals did not present any signs of acute toxicity for the EEZR (up to the 4 g/kg dose, po), and it was not possible to calculate the DL(50). EEZR (125-500 mg/kg) exhibited a significant gastroprotective effect in absolute ethanol, HCl/ethanol, hypothermic-restraint stress, and ischemia/reperfusion-induced gastric lesion models. EEZR (250 and 500 mg/kg) exhibited still a gastroprotective activity in the indomethacin-induced ulcer model. Gastroprotection of EEZR was significantly decreased in pre-treated mice with l-NAME or glibenclamide, the respective nitric oxide synthase and K(ATP) channels inhibitors. Our studies revealed that EEZR (500 mg/kg) prevented the decrease of the non-protein sulfhydril groups (NP-SH) and increased the catalase levels in ethanol-treated animals. Furthermore, the extract (500 mg/kg) significantly increased the mucus production, however, the gastric secretion parameters (volume, [H(+)], pH) did not show any alteration.

CONCLUSIONS

Our results indicate that the ethanolic extract of Zanthoxylum rhoifolium exhibits a significant gastroprotection, because it inhibits the formation of gastric lesions using different models. The release of the nitric oxide, the opening of the K(ATP) channels, the participation of the non-protein sulfhydril groups (NP-SH), catalase and the increase of mucous secretion seem to be involved in the gastroprotection activity of the EEZR. Nevertheless, this activity does not seem to be related to antisecretory mechanisms.

Authors+Show Affiliations

Medicinal Plants Research Center, Federal University of Piauí, Teresina, PI, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21723384

Citation

Freitas, F F B P., et al. "Gastroprotective Activity of Zanthoxylum Rhoifolium Lam. in Animal Models." Journal of Ethnopharmacology, vol. 137, no. 1, 2011, pp. 700-8.
Freitas FF, Fernandes HB, Piauilino CA, et al. Gastroprotective activity of Zanthoxylum rhoifolium Lam. in animal models. J Ethnopharmacol. 2011;137(1):700-8.
Freitas, F. F., Fernandes, H. B., Piauilino, C. A., Pereira, S. S., Carvalho, K. I., Chaves, M. H., Soares, P. M., Miura, L. M., Leite, J. R., Oliveira, R. C., & Oliveira, F. A. (2011). Gastroprotective activity of Zanthoxylum rhoifolium Lam. in animal models. Journal of Ethnopharmacology, 137(1), 700-8. https://doi.org/10.1016/j.jep.2011.06.026
Freitas FF, et al. Gastroprotective Activity of Zanthoxylum Rhoifolium Lam. in Animal Models. J Ethnopharmacol. 2011 Sep 1;137(1):700-8. PubMed PMID: 21723384.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Gastroprotective activity of Zanthoxylum rhoifolium Lam. in animal models. AU - Freitas,F F B P, AU - Fernandes,H B, AU - Piauilino,C A, AU - Pereira,S S, AU - Carvalho,K I M, AU - Chaves,M H, AU - Soares,P M G, AU - Miura,L M C V, AU - Leite,J R S A, AU - Oliveira,R C M, AU - Oliveira,F A, Y1 - 2011/06/22/ PY - 2011/03/31/received PY - 2011/06/14/revised PY - 2011/06/15/accepted PY - 2011/7/5/entrez PY - 2011/7/5/pubmed PY - 2011/12/28/medline SP - 700 EP - 8 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 137 IS - 1 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: The stem barks of Zanthoxylum rhoifolium Lam. (Rutaceae), locally known as "mamica de cadela", are popularly used in dyspepsies, stomachic, tonic, antitumoral, antipyretic and are used in treating flatulence and colic. The objective of this study was to evaluate the gastroprotective effect of the ethanolic extract of Zanthoxylum rhoifolium (EEZR) stem barks in acute gastric lesion models, investigating their possible mechanisms. MATERIALS AND METHODS: Mice were used for the evaluation of the acute toxicity, and mice and rats to study the gastroprotective activity. The gastroprotective action of EEZR was analyzed in the absolute ethanol, HCl/ethanol and indomethacin-induced gastric lesion models in mice, hypothermic-restraint stress, and ischemia/reperfusion in rats. In the investigation of the gastroprotective mechanisms of EEZR, the participation of the NO-synthase pathway, ATP-sensitive potassium channels (K(ATP)), the levels of the non-protein sulfhydril groups (NP-SH) and the catalase activity using the ethanol-induced gastric mucosa lesion model and the quantification of the gastric mucus and the antisecretory activity through pylorus ligature model in rats were analyzed. RESULTS: The animals did not present any signs of acute toxicity for the EEZR (up to the 4 g/kg dose, po), and it was not possible to calculate the DL(50). EEZR (125-500 mg/kg) exhibited a significant gastroprotective effect in absolute ethanol, HCl/ethanol, hypothermic-restraint stress, and ischemia/reperfusion-induced gastric lesion models. EEZR (250 and 500 mg/kg) exhibited still a gastroprotective activity in the indomethacin-induced ulcer model. Gastroprotection of EEZR was significantly decreased in pre-treated mice with l-NAME or glibenclamide, the respective nitric oxide synthase and K(ATP) channels inhibitors. Our studies revealed that EEZR (500 mg/kg) prevented the decrease of the non-protein sulfhydril groups (NP-SH) and increased the catalase levels in ethanol-treated animals. Furthermore, the extract (500 mg/kg) significantly increased the mucus production, however, the gastric secretion parameters (volume, [H(+)], pH) did not show any alteration. CONCLUSIONS: Our results indicate that the ethanolic extract of Zanthoxylum rhoifolium exhibits a significant gastroprotection, because it inhibits the formation of gastric lesions using different models. The release of the nitric oxide, the opening of the K(ATP) channels, the participation of the non-protein sulfhydril groups (NP-SH), catalase and the increase of mucous secretion seem to be involved in the gastroprotection activity of the EEZR. Nevertheless, this activity does not seem to be related to antisecretory mechanisms. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/21723384/Gastroprotective_activity_of_Zanthoxylum_rhoifolium_Lam__in_animal_models_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(11)00443-0 DB - PRIME DP - Unbound Medicine ER -