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Direct quantification of cannabinoids and cannabinoid glucuronides in whole blood by liquid chromatography-tandem mass spectrometry.
Anal Bioanal Chem. 2011 Sep; 401(4):1273-83.AB

Abstract

The first method for quantifying cannabinoids and cannabinoid glucuronides in whole blood by liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed and validated. Solid-phase extraction followed protein precipitation with acetonitrile. High-performance liquid chromatography separation was achieved in 16 min via gradient elution. Electrospray ionization was utilized for cannabinoid detection; both positive (Δ(9)-tetrahydrocannabinol [THC] and cannabinol [CBN]) and negative (11-hydroxy-THC [11-OH-THC], 11-nor-9-carboxy-THC [THCCOOH], cannabidiol [CBD], THC-glucuronide, and THCCOOH-glucuronide) polarity were employed with multiple reaction monitoring. Calibration by linear regression analysis utilized deuterium-labeled internal standards and a 1/x(2) weighting factor, yielding R(2) values >0.997 for all analytes. Linearity ranged from 0.5 to 50 μg/L (THC-glucuronide), 1.0-100 μg/L (THC, 11-OH-THC, THCCOOH, CBD, and CBN), and 5.0-250 μg/L (THCCOOH-glucuronide). Imprecision was <10.5% CV, recovery was >50.5%, and bias within ±13.1% of target for all analytes at three concentrations across the linear range. No carryover and endogenous or exogenous interferences were observed. This new analytical method should be useful for quantifying cannabinoids in whole blood and further investigating cannabinoid glucuronides as markers of recent cannabis intake.

Authors+Show Affiliations

Chemistry and Drug Metabolism, National Institute on Drug Abuse Intramural Research Program, National Institutes of Health, Baltimore, MD 21224, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Intramural
Validation Study

Language

eng

PubMed ID

21727996

Citation

Schwope, David M., et al. "Direct Quantification of Cannabinoids and Cannabinoid Glucuronides in Whole Blood By Liquid Chromatography-tandem Mass Spectrometry." Analytical and Bioanalytical Chemistry, vol. 401, no. 4, 2011, pp. 1273-83.
Schwope DM, Scheidweiler KB, Huestis MA. Direct quantification of cannabinoids and cannabinoid glucuronides in whole blood by liquid chromatography-tandem mass spectrometry. Anal Bioanal Chem. 2011;401(4):1273-83.
Schwope, D. M., Scheidweiler, K. B., & Huestis, M. A. (2011). Direct quantification of cannabinoids and cannabinoid glucuronides in whole blood by liquid chromatography-tandem mass spectrometry. Analytical and Bioanalytical Chemistry, 401(4), 1273-83. https://doi.org/10.1007/s00216-011-5197-7
Schwope DM, Scheidweiler KB, Huestis MA. Direct Quantification of Cannabinoids and Cannabinoid Glucuronides in Whole Blood By Liquid Chromatography-tandem Mass Spectrometry. Anal Bioanal Chem. 2011;401(4):1273-83. PubMed PMID: 21727996.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Direct quantification of cannabinoids and cannabinoid glucuronides in whole blood by liquid chromatography-tandem mass spectrometry. AU - Schwope,David M, AU - Scheidweiler,Karl B, AU - Huestis,Marilyn A, Y1 - 2011/07/05/ PY - 2011/05/06/received PY - 2011/06/19/accepted PY - 2011/06/14/revised PY - 2011/7/6/entrez PY - 2011/7/6/pubmed PY - 2012/1/12/medline SP - 1273 EP - 83 JF - Analytical and bioanalytical chemistry JO - Anal Bioanal Chem VL - 401 IS - 4 N2 - The first method for quantifying cannabinoids and cannabinoid glucuronides in whole blood by liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed and validated. Solid-phase extraction followed protein precipitation with acetonitrile. High-performance liquid chromatography separation was achieved in 16 min via gradient elution. Electrospray ionization was utilized for cannabinoid detection; both positive (Δ(9)-tetrahydrocannabinol [THC] and cannabinol [CBN]) and negative (11-hydroxy-THC [11-OH-THC], 11-nor-9-carboxy-THC [THCCOOH], cannabidiol [CBD], THC-glucuronide, and THCCOOH-glucuronide) polarity were employed with multiple reaction monitoring. Calibration by linear regression analysis utilized deuterium-labeled internal standards and a 1/x(2) weighting factor, yielding R(2) values >0.997 for all analytes. Linearity ranged from 0.5 to 50 μg/L (THC-glucuronide), 1.0-100 μg/L (THC, 11-OH-THC, THCCOOH, CBD, and CBN), and 5.0-250 μg/L (THCCOOH-glucuronide). Imprecision was <10.5% CV, recovery was >50.5%, and bias within ±13.1% of target for all analytes at three concentrations across the linear range. No carryover and endogenous or exogenous interferences were observed. This new analytical method should be useful for quantifying cannabinoids in whole blood and further investigating cannabinoid glucuronides as markers of recent cannabis intake. SN - 1618-2650 UR - https://www.unboundmedicine.com/medline/citation/21727996/Direct_quantification_of_cannabinoids_and_cannabinoid_glucuronides_in_whole_blood_by_liquid_chromatography_tandem_mass_spectrometry_ L2 - https://dx.doi.org/10.1007/s00216-011-5197-7 DB - PRIME DP - Unbound Medicine ER -